Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_974 and Pf1N1B4_975 are separated by 184 nucleotides Pf1N1B4_975 and Pf1N1B4_976 are separated by 3 nucleotides Pf1N1B4_976 and Pf1N1B4_977 overlap by 4 nucleotides Pf1N1B4_977 and Pf1N1B4_978 are separated by 234 nucleotides
Pf1N1B4_974 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 1,103,342 to 1,103,851
_974
Pf1N1B4_975 - Molybdenum cofactor biosynthesis protein MoaE, at 1,104,036 to 1,104,488
_975
Pf1N1B4_976 - Molybdenum cofactor biosynthesis protein MoaD, at 1,104,492 to 1,104,734
_976
Pf1N1B4_977 - Molybdenum cofactor biosynthesis protein MoaC, at 1,104,731 to 1,105,204
_977
Pf1N1B4_978 - Predicted ATPase related to phosphate starvation-inducible protein PhoH, at 1,105,439 to 1,106,833
_978
Position (kb)
1104
1105 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1103.574 kb on + strand, within Pf1N1B4_974 at 1103.574 kb on + strand, within Pf1N1B4_974 at 1103.574 kb on + strand, within Pf1N1B4_974 at 1103.575 kb on - strand, within Pf1N1B4_974 at 1103.760 kb on + strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.978 kb on + strand at 1103.979 kb on - strand at 1104.000 kb on - strand at 1104.037 kb on + strand at 1104.038 kb on - strand at 1104.038 kb on - strand at 1104.038 kb on - strand at 1104.145 kb on + strand, within Pf1N1B4_975 at 1104.145 kb on + strand, within Pf1N1B4_975 at 1104.146 kb on - strand, within Pf1N1B4_975 at 1104.146 kb on - strand, within Pf1N1B4_975 at 1104.601 kb on - strand, within Pf1N1B4_976 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.943 kb on + strand, within Pf1N1B4_977 at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.169 kb on - strand at 1105.210 kb on + strand at 1105.211 kb on - strand at 1105.235 kb on + strand at 1105.235 kb on + strand at 1105.268 kb on + strand at 1105.328 kb on + strand at 1105.335 kb on + strand at 1105.336 kb on - strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.394 kb on - strand at 1105.394 kb on - strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.569 kb on + strand at 1105.570 kb on - strand at 1105.660 kb on + strand, within Pf1N1B4_978 at 1105.660 kb on + strand, within Pf1N1B4_978 at 1105.661 kb on - strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,103,574 + Pf1N1B4_974 0.45 -0.6 1,103,574 + Pf1N1B4_974 0.45 -1.0 1,103,574 + Pf1N1B4_974 0.45 +0.6 1,103,575 - Pf1N1B4_974 0.46 -0.7 1,103,760 + Pf1N1B4_974 0.82 +0.6 1,103,761 - Pf1N1B4_974 0.82 +1.1 1,103,761 - Pf1N1B4_974 0.82 +1.1 1,103,761 - Pf1N1B4_974 0.82 +0.5 1,103,897 - -1.7 1,103,897 - -0.6 1,103,897 - -0.4 1,103,897 - +1.6 1,103,897 - -0.4 1,103,897 - -0.7 1,103,897 - -1.1 1,103,897 - -0.7 1,103,897 - -1.6 1,103,978 + +0.0 1,103,979 - +1.6 1,104,000 - -1.4 1,104,037 + +0.6 1,104,038 - +0.2 1,104,038 - +1.6 1,104,038 - -0.6 1,104,145 + Pf1N1B4_975 0.24 -0.9 1,104,145 + Pf1N1B4_975 0.24 +0.0 1,104,146 - Pf1N1B4_975 0.24 -1.4 1,104,146 - Pf1N1B4_975 0.24 -1.0 1,104,601 - Pf1N1B4_976 0.45 -1.7 1,104,834 + Pf1N1B4_977 0.22 -1.2 1,104,834 + Pf1N1B4_977 0.22 -2.2 1,104,834 + Pf1N1B4_977 0.22 -2.3 1,104,834 + Pf1N1B4_977 0.22 -0.5 1,104,834 + Pf1N1B4_977 0.22 -2.4 1,104,834 + Pf1N1B4_977 0.22 -2.1 1,104,834 + Pf1N1B4_977 0.22 -1.5 1,104,835 - Pf1N1B4_977 0.22 -1.9 1,104,835 - Pf1N1B4_977 0.22 -1.2 1,104,835 - Pf1N1B4_977 0.22 -2.8 1,104,835 - Pf1N1B4_977 0.22 -0.4 1,104,835 - Pf1N1B4_977 0.22 +0.0 1,104,943 + Pf1N1B4_977 0.45 +0.6 1,105,168 + -1.0 1,105,168 + -2.1 1,105,168 + -2.0 1,105,168 + -2.9 1,105,168 + -1.4 1,105,168 + -0.4 1,105,169 - -0.7 1,105,210 + -2.0 1,105,211 - -2.3 1,105,235 + +0.9 1,105,235 + +0.6 1,105,268 + +0.3 1,105,328 + +0.0 1,105,335 + +0.6 1,105,336 - -0.4 1,105,380 + +2.0 1,105,380 + +1.6 1,105,380 + +0.0 1,105,380 + +1.1 1,105,380 + -1.6 1,105,384 + +0.8 1,105,384 + +1.3 1,105,384 + +0.8 1,105,384 + -4.0 1,105,384 + -0.4 1,105,384 + +1.6 1,105,384 + +0.7 1,105,385 - +1.1 1,105,385 - -0.2 1,105,385 - -1.0 1,105,385 - +1.0 1,105,385 - -1.2 1,105,385 - -1.5 1,105,385 - -1.1 1,105,385 - -0.5 1,105,394 - +2.0 1,105,394 - +0.0 1,105,502 + -1.1 1,105,502 + -0.6 1,105,502 + +0.3 1,105,502 + -0.8 1,105,502 + -0.1 1,105,502 + +0.0 1,105,502 + +0.4 1,105,503 - +0.4 1,105,503 - +0.1 1,105,503 - +2.3 1,105,503 - +1.2 1,105,503 - +0.6 1,105,503 - +3.2 1,105,503 - +0.0 1,105,539 + +1.4 1,105,539 + +0.3 1,105,539 + +0.0 1,105,539 + -0.0 1,105,539 + +0.4 1,105,540 - +2.4 1,105,540 - -1.5 1,105,540 - -1.0 1,105,540 - +0.0 1,105,540 - +1.6 1,105,540 - +0.0 1,105,540 - -0.7 1,105,569 + +1.0 1,105,570 - -0.6 1,105,660 + Pf1N1B4_978 0.16 +1.0 1,105,660 + Pf1N1B4_978 0.16 -2.0 1,105,661 - Pf1N1B4_978 0.16 +1.2 1,105,719 + Pf1N1B4_978 0.20 -0.6 1,105,719 + Pf1N1B4_978 0.20 -0.5 1,105,719 + Pf1N1B4_978 0.20 -0.4 1,105,719 + Pf1N1B4_978 0.20 +0.0 1,105,719 + Pf1N1B4_978 0.20 -0.2 1,105,719 + Pf1N1B4_978 0.20 +1.4 1,105,719 + Pf1N1B4_978 0.20 +2.6 1,105,719 + Pf1N1B4_978 0.20 -0.3 1,105,719 + Pf1N1B4_978 0.20 -0.1 1,105,719 + Pf1N1B4_978 0.20 -0.5 1,105,720 - Pf1N1B4_978 0.20 -0.7 1,105,720 - Pf1N1B4_978 0.20 +0.4 1,105,720 - Pf1N1B4_978 0.20 +1.4 1,105,720 - +1.6 1,105,720 - Pf1N1B4_978 0.20 +1.0 1,105,720 - Pf1N1B4_978 0.20 +0.2
Or see this region's nucleotide sequence