Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_857

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_855 and Pf1N1B4_856 overlap by 1 nucleotidesPf1N1B4_856 and Pf1N1B4_857 are separated by 12 nucleotidesPf1N1B4_857 and Pf1N1B4_858 are separated by 28 nucleotidesPf1N1B4_858 and Pf1N1B4_859 are separated by 92 nucleotidesPf1N1B4_859 and Pf1N1B4_860 are separated by 14 nucleotides Pf1N1B4_855 - FIG021952: putative membrane protein, at 984,780 to 985,820 _855 Pf1N1B4_856 - Type IV pilus biogenesis protein PilF, at 985,820 to 986,578 _856 Pf1N1B4_857 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 986,591 to 987,739 _857 Pf1N1B4_858 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 987,768 to 988,193 _858 Pf1N1B4_859 - Believed to be involved in assembly of Fe-S clusters, at 988,286 to 988,486 _859 Pf1N1B4_860 - Ferredoxin, 2Fe-2S, at 988,501 to 988,842 _860 Position (kb) 986 987 988Strain fitness (log2 ratio) -1 0 1 2at 985.837 kb on - strandat 985.846 kb on - strandat 985.846 kb on - strandat 985.944 kb on - strand, within Pf1N1B4_856at 985.944 kb on - strand, within Pf1N1B4_856at 985.944 kb on - strandat 985.949 kb on - strand, within Pf1N1B4_856at 985.987 kb on - strand, within Pf1N1B4_856at 985.990 kb on - strand, within Pf1N1B4_856at 985.990 kb on - strand, within Pf1N1B4_856at 986.007 kb on + strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.008 kb on - strand, within Pf1N1B4_856at 986.023 kb on - strand, within Pf1N1B4_856at 986.023 kb on - strand, within Pf1N1B4_856at 986.439 kb on + strand, within Pf1N1B4_856at 986.461 kb on - strand, within Pf1N1B4_856at 986.578 kb on + strandat 986.578 kb on + strandat 986.578 kb on + strandat 986.579 kb on - strandat 986.579 kb on - strandat 986.579 kb on - strandat 986.579 kb on - strandat 986.579 kb on - strandat 986.592 kb on + strandat 986.592 kb on + strandat 986.592 kb on + strandat 986.592 kb on + strandat 986.592 kb on + strandat 986.593 kb on - strandat 986.593 kb on - strandat 986.593 kb on - strandat 986.596 kb on + strandat 986.596 kb on + strandat 986.597 kb on - strandat 986.597 kb on - strandat 986.597 kb on - strandat 986.656 kb on - strandat 986.656 kb on - strandat 986.935 kb on - strand, within Pf1N1B4_857at 988.229 kb on - strandat 988.260 kb on - strandat 988.411 kb on - strand, within Pf1N1B4_859at 988.411 kb on - strand, within Pf1N1B4_859

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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985,837 - +0.7
985,846 - -1.3
985,846 - +2.6
985,944 - Pf1N1B4_856 0.16 -0.8
985,944 - Pf1N1B4_856 0.16 +0.7
985,944 - +0.6
985,949 - Pf1N1B4_856 0.17 +0.1
985,987 - Pf1N1B4_856 0.22 -0.4
985,990 - Pf1N1B4_856 0.22 +1.0
985,990 - Pf1N1B4_856 0.22 -0.0
986,007 + Pf1N1B4_856 0.25 +0.3
986,008 - Pf1N1B4_856 0.25 +1.3
986,008 - Pf1N1B4_856 0.25 -0.2
986,008 - Pf1N1B4_856 0.25 -0.0
986,008 - Pf1N1B4_856 0.25 -0.4
986,008 - Pf1N1B4_856 0.25 +0.6
986,008 - Pf1N1B4_856 0.25 -0.2
986,008 - Pf1N1B4_856 0.25 +2.6
986,008 - Pf1N1B4_856 0.25 -1.1
986,008 - Pf1N1B4_856 0.25 -0.7
986,023 - Pf1N1B4_856 0.27 -0.5
986,023 - Pf1N1B4_856 0.27 +0.2
986,439 + Pf1N1B4_856 0.82 +1.2
986,461 - Pf1N1B4_856 0.84 +0.5
986,578 + +1.2
986,578 + -0.9
986,578 + +0.4
986,579 - +1.6
986,579 - +1.4
986,579 - +1.0
986,579 - +0.2
986,579 - -0.2
986,592 + +1.6
986,592 + +0.6
986,592 + +1.3
986,592 + -0.2
986,592 + +0.6
986,593 - -0.2
986,593 - -0.9
986,593 - -0.4
986,596 + +0.2
986,596 + +0.3
986,597 - +1.3
986,597 - +0.7
986,597 - +1.9
986,656 - -0.0
986,656 - +0.3
986,935 - Pf1N1B4_857 0.30 -1.3
988,229 - +0.3
988,260 - +0.8
988,411 - Pf1N1B4_859 0.62 -1.2
988,411 - Pf1N1B4_859 0.62 -1.7

Or see this region's nucleotide sequence