Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_855 and Pf1N1B4_856 overlap by 1 nucleotides Pf1N1B4_856 and Pf1N1B4_857 are separated by 12 nucleotides Pf1N1B4_857 and Pf1N1B4_858 are separated by 28 nucleotides Pf1N1B4_858 and Pf1N1B4_859 are separated by 92 nucleotides Pf1N1B4_859 and Pf1N1B4_860 are separated by 14 nucleotides
Pf1N1B4_855 - FIG021952: putative membrane protein, at 984,780 to 985,820
_855
Pf1N1B4_856 - Type IV pilus biogenesis protein PilF, at 985,820 to 986,578
_856
Pf1N1B4_857 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 986,591 to 987,739
_857
Pf1N1B4_858 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 987,768 to 988,193
_858
Pf1N1B4_859 - Believed to be involved in assembly of Fe-S clusters, at 988,286 to 988,486
_859
Pf1N1B4_860 - Ferredoxin, 2Fe-2S, at 988,501 to 988,842
_860
Position (kb)
986
987
988 Strain fitness (log2 ratio)
-1
0
1
2 at 985.837 kb on - strand at 985.846 kb on - strand at 985.846 kb on - strand at 985.944 kb on - strand, within Pf1N1B4_856 at 985.944 kb on - strand, within Pf1N1B4_856 at 985.944 kb on - strand at 985.949 kb on - strand, within Pf1N1B4_856 at 985.987 kb on - strand, within Pf1N1B4_856 at 985.990 kb on - strand, within Pf1N1B4_856 at 985.990 kb on - strand, within Pf1N1B4_856 at 986.007 kb on + strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.023 kb on - strand, within Pf1N1B4_856 at 986.023 kb on - strand, within Pf1N1B4_856 at 986.439 kb on + strand, within Pf1N1B4_856 at 986.461 kb on - strand, within Pf1N1B4_856 at 986.578 kb on + strand at 986.578 kb on + strand at 986.578 kb on + strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.593 kb on - strand at 986.593 kb on - strand at 986.593 kb on - strand at 986.596 kb on + strand at 986.596 kb on + strand at 986.597 kb on - strand at 986.597 kb on - strand at 986.597 kb on - strand at 986.656 kb on - strand at 986.656 kb on - strand at 986.935 kb on - strand, within Pf1N1B4_857 at 988.229 kb on - strand at 988.260 kb on - strand at 988.411 kb on - strand, within Pf1N1B4_859 at 988.411 kb on - strand, within Pf1N1B4_859
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 985,837 - +0.7 985,846 - -1.3 985,846 - +2.6 985,944 - Pf1N1B4_856 0.16 -0.8 985,944 - Pf1N1B4_856 0.16 +0.7 985,944 - +0.6 985,949 - Pf1N1B4_856 0.17 +0.1 985,987 - Pf1N1B4_856 0.22 -0.4 985,990 - Pf1N1B4_856 0.22 +1.0 985,990 - Pf1N1B4_856 0.22 -0.0 986,007 + Pf1N1B4_856 0.25 +0.3 986,008 - Pf1N1B4_856 0.25 +1.3 986,008 - Pf1N1B4_856 0.25 -0.2 986,008 - Pf1N1B4_856 0.25 -0.0 986,008 - Pf1N1B4_856 0.25 -0.4 986,008 - Pf1N1B4_856 0.25 +0.6 986,008 - Pf1N1B4_856 0.25 -0.2 986,008 - Pf1N1B4_856 0.25 +2.6 986,008 - Pf1N1B4_856 0.25 -1.1 986,008 - Pf1N1B4_856 0.25 -0.7 986,023 - Pf1N1B4_856 0.27 -0.5 986,023 - Pf1N1B4_856 0.27 +0.2 986,439 + Pf1N1B4_856 0.82 +1.2 986,461 - Pf1N1B4_856 0.84 +0.5 986,578 + +1.2 986,578 + -0.9 986,578 + +0.4 986,579 - +1.6 986,579 - +1.4 986,579 - +1.0 986,579 - +0.2 986,579 - -0.2 986,592 + +1.6 986,592 + +0.6 986,592 + +1.3 986,592 + -0.2 986,592 + +0.6 986,593 - -0.2 986,593 - -0.9 986,593 - -0.4 986,596 + +0.2 986,596 + +0.3 986,597 - +1.3 986,597 - +0.7 986,597 - +1.9 986,656 - -0.0 986,656 - +0.3 986,935 - Pf1N1B4_857 0.30 -1.3 988,229 - +0.3 988,260 - +0.8 988,411 - Pf1N1B4_859 0.62 -1.2 988,411 - Pf1N1B4_859 0.62 -1.7
Or see this region's nucleotide sequence