Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5755 and Pf1N1B4_5756 are separated by 592 nucleotides Pf1N1B4_5756 and Pf1N1B4_5757 overlap by 4 nucleotides Pf1N1B4_5757 and Pf1N1B4_5758 are separated by 195 nucleotides
Pf1N1B4_5755 - NnrS protein involved in response to NO, at 195,067 to 195,600
_5755
Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876
_5756
Pf1N1B4_5757 - hypothetical protein, at 196,873 to 197,067
_5757
Pf1N1B4_5758 - Nitric oxide reductase activation protein NorD, at 197,263 to 199,104
_5758
Position (kb) 196
197 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 195.615 kb on - strand at 195.655 kb on + strand at 195.656 kb on - strand at 195.656 kb on - strand at 195.926 kb on + strand at 195.927 kb on - strand at 196.142 kb on + strand at 196.352 kb on + strand, within Pf1N1B4_5756 at 196.353 kb on - strand, within Pf1N1B4_5756 at 196.479 kb on - strand, within Pf1N1B4_5756 at 196.538 kb on + strand, within Pf1N1B4_5756 at 196.539 kb on - strand, within Pf1N1B4_5756 at 196.689 kb on + strand, within Pf1N1B4_5756 at 196.897 kb on + strand, within Pf1N1B4_5757 at 196.897 kb on + strand, within Pf1N1B4_5757 at 197.078 kb on + strand at 197.078 kb on + strand at 197.079 kb on - strand at 197.079 kb on - strand at 197.237 kb on + strand at 197.308 kb on + strand at 197.309 kb on - strand at 197.357 kb on + strand at 197.357 kb on + strand at 197.358 kb on - strand at 197.366 kb on + strand at 197.394 kb on + strand at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.472 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.585 kb on + strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.589 kb on - strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.765 kb on - strand, within Pf1N1B4_5758
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 195,615 - -0.6 195,655 + -2.1 195,656 - +0.5 195,656 - -1.8 195,926 + -0.5 195,927 - -1.8 196,142 + -0.3 196,352 + Pf1N1B4_5756 0.23 -2.6 196,353 - Pf1N1B4_5756 0.23 -2.3 196,479 - Pf1N1B4_5756 0.42 -0.9 196,538 + Pf1N1B4_5756 0.50 -1.5 196,539 - Pf1N1B4_5756 0.51 +0.3 196,689 + Pf1N1B4_5756 0.73 +1.1 196,897 + Pf1N1B4_5757 0.12 -2.2 196,897 + Pf1N1B4_5757 0.12 -2.1 197,078 + -0.9 197,078 + +0.5 197,079 - -0.7 197,079 - +0.8 197,237 + -1.3 197,308 + -1.6 197,309 - -1.4 197,357 + +0.6 197,357 + -1.3 197,358 - -1.2 197,366 + -1.1 197,394 + -1.2 197,471 + Pf1N1B4_5758 0.11 -0.1 197,471 + Pf1N1B4_5758 0.11 -2.6 197,472 - Pf1N1B4_5758 0.11 +0.0 197,546 - Pf1N1B4_5758 0.15 -2.4 197,546 - Pf1N1B4_5758 0.15 -1.7 197,585 + Pf1N1B4_5758 0.17 -1.1 197,586 - Pf1N1B4_5758 0.18 -1.4 197,586 - Pf1N1B4_5758 0.18 -1.2 197,586 - Pf1N1B4_5758 0.18 -2.3 197,589 - Pf1N1B4_5758 0.18 -0.4 197,764 + Pf1N1B4_5758 0.27 +0.7 197,764 + Pf1N1B4_5758 0.27 -1.9 197,764 + Pf1N1B4_5758 0.27 +0.0 197,765 - Pf1N1B4_5758 0.27 -4.3
Or see this region's nucleotide sequence