Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotides Pf1N1B4_5620 and Pf1N1B4_5621 are separated by 0 nucleotides Pf1N1B4_5621 and Pf1N1B4_5622 are separated by 629 nucleotides
Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441
_5619
Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069
_5620
Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828
_5621
Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546
_5622
Position (kb)
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 46.128 kb on + strand at 46.128 kb on + strand, within Pf1N1B4_5619 at 46.129 kb on - strand, within Pf1N1B4_5619 at 46.485 kb on - strand at 46.493 kb on - strand at 46.522 kb on + strand at 46.580 kb on + strand at 46.647 kb on - strand at 46.837 kb on - strand, within Pf1N1B4_5620 at 46.856 kb on - strand, within Pf1N1B4_5620 at 46.889 kb on - strand, within Pf1N1B4_5620 at 47.135 kb on + strand at 47.136 kb on - strand at 47.273 kb on - strand, within Pf1N1B4_5621 at 47.766 kb on - strand at 47.891 kb on + strand at 47.958 kb on + strand at 47.958 kb on + strand at 48.084 kb on + strand at 48.084 kb on + strand at 48.140 kb on + strand at 48.140 kb on + strand at 48.262 kb on + strand at 48.262 kb on + strand at 48.271 kb on + strand at 48.271 kb on + strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.307 kb on - strand at 48.559 kb on - strand at 48.761 kb on + strand, within Pf1N1B4_5622 at 48.807 kb on - strand, within Pf1N1B4_5622
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 46,128 + +5.5 46,128 + Pf1N1B4_5619 0.46 +5.4 46,129 - Pf1N1B4_5619 0.46 +5.1 46,485 - +5.7 46,493 - +4.8 46,522 + +5.5 46,580 + +0.5 46,647 - -2.1 46,837 - Pf1N1B4_5620 0.48 +2.1 46,856 - Pf1N1B4_5620 0.52 -1.3 46,889 - Pf1N1B4_5620 0.59 +0.6 47,135 + +0.4 47,136 - +2.0 47,273 - Pf1N1B4_5621 0.27 -1.2 47,766 - +0.4 47,891 + +0.3 47,958 + -1.0 47,958 + +1.3 48,084 + +0.6 48,084 + +0.3 48,140 + -0.6 48,140 + +0.9 48,262 + -1.2 48,262 + +0.9 48,271 + -0.4 48,271 + +0.7 48,272 - -0.8 48,272 - -2.1 48,272 - -0.7 48,307 - +1.6 48,559 - -0.6 48,761 + Pf1N1B4_5622 0.28 -0.3 48,807 - Pf1N1B4_5622 0.32 +0.4
Or see this region's nucleotide sequence