Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5621

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotidesPf1N1B4_5620 and Pf1N1B4_5621 are separated by 0 nucleotidesPf1N1B4_5621 and Pf1N1B4_5622 are separated by 629 nucleotides Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441 _5619 Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069 _5620 Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828 _5621 Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546 _5622 Position (kb) 47 48Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 46.128 kb on + strandat 46.128 kb on + strand, within Pf1N1B4_5619at 46.129 kb on - strand, within Pf1N1B4_5619at 46.485 kb on - strandat 46.493 kb on - strandat 46.522 kb on + strandat 46.580 kb on + strandat 46.647 kb on - strandat 46.837 kb on - strand, within Pf1N1B4_5620at 46.856 kb on - strand, within Pf1N1B4_5620at 46.889 kb on - strand, within Pf1N1B4_5620at 47.135 kb on + strandat 47.136 kb on - strandat 47.273 kb on - strand, within Pf1N1B4_5621at 47.766 kb on - strandat 47.891 kb on + strandat 47.958 kb on + strandat 47.958 kb on + strandat 48.084 kb on + strandat 48.084 kb on + strandat 48.140 kb on + strandat 48.140 kb on + strandat 48.262 kb on + strandat 48.262 kb on + strandat 48.271 kb on + strandat 48.271 kb on + strandat 48.272 kb on - strandat 48.272 kb on - strandat 48.272 kb on - strandat 48.307 kb on - strandat 48.559 kb on - strandat 48.761 kb on + strand, within Pf1N1B4_5622at 48.807 kb on - strand, within Pf1N1B4_5622

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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46,128 + +5.5
46,128 + Pf1N1B4_5619 0.46 +5.4
46,129 - Pf1N1B4_5619 0.46 +5.1
46,485 - +5.7
46,493 - +4.8
46,522 + +5.5
46,580 + +0.5
46,647 - -2.1
46,837 - Pf1N1B4_5620 0.48 +2.1
46,856 - Pf1N1B4_5620 0.52 -1.3
46,889 - Pf1N1B4_5620 0.59 +0.6
47,135 + +0.4
47,136 - +2.0
47,273 - Pf1N1B4_5621 0.27 -1.2
47,766 - +0.4
47,891 + +0.3
47,958 + -1.0
47,958 + +1.3
48,084 + +0.6
48,084 + +0.3
48,140 + -0.6
48,140 + +0.9
48,262 + -1.2
48,262 + +0.9
48,271 + -0.4
48,271 + +0.7
48,272 - -0.8
48,272 - -2.1
48,272 - -0.7
48,307 - +1.6
48,559 - -0.6
48,761 + Pf1N1B4_5622 0.28 -0.3
48,807 - Pf1N1B4_5622 0.32 +0.4

Or see this region's nucleotide sequence