Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5262 and Pf1N1B4_5263 are separated by 491 nucleotides Pf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotides Pf1N1B4_5264 and Pf1N1B4_5265 are separated by 299 nucleotides Pf1N1B4_5265 and Pf1N1B4_5266 are separated by 21 nucleotides
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723
_5263
Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868
_5264
Pf1N1B4_5265 - hypothetical protein, at 23,168 to 23,296
_5265
Pf1N1B4_5266 - hypothetical protein, at 23,318 to 23,638
_5266
Position (kb)
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.527 kb on + strand at 22.528 kb on - strand at 22.541 kb on + strand at 22.541 kb on + strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.670 kb on + strand, within Pf1N1B4_5263 at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.671 kb on - strand at 22.671 kb on - strand at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.718 kb on - strand at 22.718 kb on - strand at 22.797 kb on + strand, within Pf1N1B4_5264 at 22.798 kb on - strand at 22.878 kb on + strand at 22.879 kb on - strand at 22.879 kb on - strand at 22.905 kb on - strand at 22.906 kb on + strand at 22.907 kb on - strand at 22.907 kb on - strand at 22.938 kb on - strand at 22.958 kb on - strand at 22.974 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.077 kb on - strand at 23.077 kb on - strand at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.251 kb on + strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.312 kb on + strand at 23.312 kb on + strand at 23.313 kb on - strand at 23.313 kb on - strand at 23.398 kb on + strand, within Pf1N1B4_5266 at 23.399 kb on - strand, within Pf1N1B4_5266 at 23.533 kb on - strand, within Pf1N1B4_5266 at 23.533 kb on - strand, within Pf1N1B4_5266 at 23.686 kb on + strand at 23.687 kb on - strand at 23.693 kb on - strand at 23.810 kb on + strand at 23.840 kb on + strand at 23.840 kb on + strand at 23.841 kb on - strand at 23.841 kb on - strand at 23.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 21,904 + Pf1N1B4_5262 0.73 +0.1 21,904 + Pf1N1B4_5262 0.73 -2.7 21,904 + Pf1N1B4_5262 0.73 -2.0 21,905 - Pf1N1B4_5262 0.73 +0.5 21,905 - Pf1N1B4_5262 0.73 +0.5 21,905 - Pf1N1B4_5262 0.73 +0.0 21,905 - Pf1N1B4_5262 0.73 +2.5 21,941 + Pf1N1B4_5262 0.81 -1.2 21,941 + Pf1N1B4_5262 0.81 -1.8 21,943 + Pf1N1B4_5262 0.81 -0.7 21,944 - Pf1N1B4_5262 0.81 -0.5 21,944 - Pf1N1B4_5262 0.81 +3.3 21,961 + Pf1N1B4_5262 0.85 +0.9 22,078 - +0.8 22,104 + +0.0 22,105 - -0.5 22,105 - -1.4 22,159 + -0.7 22,159 + -0.5 22,160 - +0.3 22,162 + -1.3 22,162 + +0.0 22,162 + +0.6 22,162 + +2.5 22,162 + +0.1 22,527 + +1.6 22,528 - -0.8 22,541 + -0.8 22,541 + +1.8 22,542 - +0.1 22,542 - -0.6 22,542 - +1.2 22,542 - -1.5 22,604 + Pf1N1B4_5263 0.39 +0.3 22,604 + Pf1N1B4_5263 0.39 +0.6 22,605 - Pf1N1B4_5263 0.40 -1.6 22,605 - Pf1N1B4_5263 0.40 -1.8 22,605 - Pf1N1B4_5263 0.40 -2.2 22,605 - Pf1N1B4_5263 0.40 +0.6 22,605 - Pf1N1B4_5263 0.40 +1.8 22,605 - Pf1N1B4_5263 0.40 -0.1 22,605 - Pf1N1B4_5263 0.40 -1.2 22,605 - Pf1N1B4_5263 0.40 -1.0 22,605 - Pf1N1B4_5263 0.40 -0.3 22,605 - Pf1N1B4_5263 0.40 -0.7 22,670 + Pf1N1B4_5263 0.73 +1.9 22,671 - Pf1N1B4_5263 0.73 +3.1 22,671 - -0.8 22,671 - +0.1 22,671 - Pf1N1B4_5263 0.73 -0.2 22,718 - -0.7 22,718 - +0.5 22,797 + Pf1N1B4_5264 0.47 +1.3 22,798 - -0.1 22,878 + -1.0 22,879 - -1.3 22,879 - -0.8 22,905 - -0.8 22,906 + -1.0 22,907 - +1.6 22,907 - +0.3 22,938 - +0.2 22,958 - +0.7 22,974 - +0.3 23,042 - -0.1 23,042 - +0.1 23,042 - +1.5 23,042 - +0.9 23,042 - +0.5 23,077 - +2.0 23,077 - +0.2 23,211 - Pf1N1B4_5265 0.33 -0.2 23,211 - Pf1N1B4_5265 0.33 +0.7 23,211 - Pf1N1B4_5265 0.33 -0.7 23,251 + Pf1N1B4_5265 0.64 +0.4 23,252 - Pf1N1B4_5265 0.65 -0.5 23,252 - Pf1N1B4_5265 0.65 +0.7 23,252 - Pf1N1B4_5265 0.65 -0.7 23,252 - Pf1N1B4_5265 0.65 -1.3 23,252 - Pf1N1B4_5265 0.65 -0.8 23,312 + -0.3 23,312 + +0.5 23,313 - -0.0 23,313 - +2.5 23,398 + Pf1N1B4_5266 0.25 +0.5 23,399 - Pf1N1B4_5266 0.25 -0.8 23,533 - Pf1N1B4_5266 0.67 -0.2 23,533 - Pf1N1B4_5266 0.67 -0.4 23,686 + -0.8 23,687 - -1.0 23,693 - +0.1 23,810 + +0.1 23,840 + +0.6 23,840 + +0.2 23,841 - -0.3 23,841 - +1.0 23,841 - -0.1
Or see this region's nucleotide sequence