Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_522 and Pf1N1B4_523 are separated by 114 nucleotides Pf1N1B4_523 and Pf1N1B4_524 are separated by 4 nucleotides
Pf1N1B4_522 - HlyD family secretion protein, at 621,288 to 622,652
_522
Pf1N1B4_523 - FIG00955751: hypothetical protein, at 622,767 to 623,360
_523
Pf1N1B4_524 - Transcriptional regulator GbuR, at 623,365 to 624,258
_524
Position (kb) 622
623
624 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 621.786 kb on + strand, within Pf1N1B4_522 at 622.392 kb on + strand, within Pf1N1B4_522 at 622.398 kb on + strand at 622.407 kb on + strand, within Pf1N1B4_522 at 622.408 kb on - strand, within Pf1N1B4_522 at 622.480 kb on - strand, within Pf1N1B4_522 at 622.480 kb on - strand at 622.571 kb on + strand at 622.571 kb on + strand at 622.571 kb on + strand at 622.599 kb on + strand at 622.599 kb on + strand at 622.608 kb on - strand at 623.062 kb on - strand, within Pf1N1B4_523 at 623.083 kb on + strand, within Pf1N1B4_523 at 623.083 kb on + strand, within Pf1N1B4_523 at 623.084 kb on - strand, within Pf1N1B4_523 at 623.366 kb on + strand at 623.366 kb on + strand at 623.367 kb on - strand at 623.367 kb on - strand at 623.494 kb on + strand, within Pf1N1B4_524 at 623.494 kb on + strand, within Pf1N1B4_524 at 623.494 kb on + strand, within Pf1N1B4_524 at 623.525 kb on + strand, within Pf1N1B4_524 at 623.541 kb on - strand, within Pf1N1B4_524 at 623.549 kb on + strand, within Pf1N1B4_524 at 623.549 kb on + strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.587 kb on - strand, within Pf1N1B4_524 at 623.694 kb on + strand, within Pf1N1B4_524 at 623.695 kb on - strand at 623.765 kb on + strand, within Pf1N1B4_524 at 623.765 kb on + strand, within Pf1N1B4_524 at 623.765 kb on + strand, within Pf1N1B4_524 at 623.766 kb on - strand, within Pf1N1B4_524 at 623.766 kb on - strand, within Pf1N1B4_524 at 623.846 kb on + strand, within Pf1N1B4_524 at 623.846 kb on + strand, within Pf1N1B4_524 at 623.897 kb on + strand, within Pf1N1B4_524 at 624.002 kb on + strand at 624.002 kb on + strand, within Pf1N1B4_524 at 624.002 kb on + strand, within Pf1N1B4_524 at 624.003 kb on - strand, within Pf1N1B4_524 at 624.140 kb on + strand, within Pf1N1B4_524 at 624.140 kb on + strand, within Pf1N1B4_524 at 624.140 kb on + strand, within Pf1N1B4_524 at 624.141 kb on - strand, within Pf1N1B4_524 at 624.141 kb on - strand, within Pf1N1B4_524 at 624.141 kb on - strand, within Pf1N1B4_524 at 624.141 kb on - strand, within Pf1N1B4_524 at 624.229 kb on + strand at 624.229 kb on + strand at 624.277 kb on - strand at 624.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 621,786 + Pf1N1B4_522 0.36 -0.3 622,392 + Pf1N1B4_522 0.81 +0.5 622,398 + +0.5 622,407 + Pf1N1B4_522 0.82 +1.5 622,408 - Pf1N1B4_522 0.82 +0.2 622,480 - Pf1N1B4_522 0.87 -0.5 622,480 - -1.5 622,571 + +0.1 622,571 + +1.5 622,571 + +3.9 622,599 + +2.5 622,599 + +0.7 622,608 - +0.4 623,062 - Pf1N1B4_523 0.50 -1.2 623,083 + Pf1N1B4_523 0.53 +0.1 623,083 + Pf1N1B4_523 0.53 +1.0 623,084 - Pf1N1B4_523 0.53 +0.5 623,366 + -1.8 623,366 + +0.3 623,367 - -0.0 623,367 - -0.3 623,494 + Pf1N1B4_524 0.14 +0.1 623,494 + Pf1N1B4_524 0.14 -1.0 623,494 + Pf1N1B4_524 0.14 -0.0 623,525 + Pf1N1B4_524 0.18 -0.3 623,541 - Pf1N1B4_524 0.20 -0.0 623,549 + Pf1N1B4_524 0.21 -1.3 623,549 + Pf1N1B4_524 0.21 +3.5 623,550 - Pf1N1B4_524 0.21 +0.8 623,550 - Pf1N1B4_524 0.21 +0.8 623,550 - Pf1N1B4_524 0.21 +2.0 623,550 - Pf1N1B4_524 0.21 -0.3 623,586 + Pf1N1B4_524 0.25 -2.4 623,586 + Pf1N1B4_524 0.25 -1.0 623,586 + Pf1N1B4_524 0.25 +1.3 623,586 + Pf1N1B4_524 0.25 -0.3 623,587 - Pf1N1B4_524 0.25 -0.8 623,694 + Pf1N1B4_524 0.37 +1.1 623,695 - +0.5 623,765 + Pf1N1B4_524 0.45 +3.1 623,765 + Pf1N1B4_524 0.45 -3.0 623,765 + Pf1N1B4_524 0.45 +1.8 623,766 - Pf1N1B4_524 0.45 -0.6 623,766 - Pf1N1B4_524 0.45 -0.5 623,846 + Pf1N1B4_524 0.54 +0.3 623,846 + Pf1N1B4_524 0.54 +0.1 623,897 + Pf1N1B4_524 0.60 -1.3 624,002 + +1.1 624,002 + Pf1N1B4_524 0.71 -0.0 624,002 + Pf1N1B4_524 0.71 +0.4 624,003 - Pf1N1B4_524 0.71 +0.3 624,140 + Pf1N1B4_524 0.87 +0.9 624,140 + Pf1N1B4_524 0.87 -1.3 624,140 + Pf1N1B4_524 0.87 +2.5 624,141 - Pf1N1B4_524 0.87 -0.2 624,141 - Pf1N1B4_524 0.87 +0.7 624,141 - Pf1N1B4_524 0.87 -0.0 624,141 - Pf1N1B4_524 0.87 -0.9 624,229 + -0.6 624,229 + +1.0 624,277 - -1.9 624,277 - -0.6
Or see this region's nucleotide sequence