Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4804

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4803 and Pf1N1B4_4804 are separated by 92 nucleotidesPf1N1B4_4804 and Pf1N1B4_4805 are separated by 10 nucleotides Pf1N1B4_4803 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 5,306,262 to 5,307,047 _4803 Pf1N1B4_4804 - ABC-type polar amino acid transport system protein, ATP-binding protein, at 5,307,140 to 5,307,904 _4804 Pf1N1B4_4805 - ABC transporter permease protein, at 5,307,915 to 5,308,793 _4805 Position (kb) 5307 5308Strain fitness (log2 ratio) -2 -1 0 1 2at 5306.208 kb on + strandat 5306.209 kb on - strandat 5306.209 kb on - strandat 5306.209 kb on - strandat 5306.209 kb on - strandat 5306.209 kb on - strandat 5306.209 kb on - strandat 5306.263 kb on + strandat 5306.264 kb on - strandat 5306.264 kb on - strandat 5306.264 kb on - strandat 5306.264 kb on - strandat 5306.264 kb on - strandat 5306.264 kb on - strandat 5306.455 kb on + strand, within Pf1N1B4_4803at 5306.456 kb on - strand, within Pf1N1B4_4803at 5306.456 kb on - strand, within Pf1N1B4_4803at 5306.456 kb on - strand, within Pf1N1B4_4803at 5306.744 kb on - strand, within Pf1N1B4_4803at 5307.061 kb on - strandat 5307.075 kb on + strandat 5307.076 kb on - strandat 5307.076 kb on - strandat 5307.076 kb on - strandat 5307.110 kb on + strandat 5307.111 kb on - strandat 5307.191 kb on + strandat 5307.191 kb on + strandat 5307.192 kb on - strandat 5307.192 kb on - strandat 5307.194 kb on + strandat 5307.194 kb on + strandat 5307.868 kb on - strandat 5307.868 kb on - strandat 5307.868 kb on - strandat 5307.868 kb on - strandat 5308.045 kb on + strand, within Pf1N1B4_4805at 5308.048 kb on + strand, within Pf1N1B4_4805at 5308.049 kb on - strandat 5308.049 kb on - strand, within Pf1N1B4_4805at 5308.049 kb on - strandat 5308.066 kb on + strand, within Pf1N1B4_4805at 5308.067 kb on - strand, within Pf1N1B4_4805at 5308.280 kb on - strand, within Pf1N1B4_4805at 5308.451 kb on - strand, within Pf1N1B4_4805at 5308.451 kb on - strand, within Pf1N1B4_4805at 5308.451 kb on - strand, within Pf1N1B4_4805at 5308.577 kb on + strand, within Pf1N1B4_4805at 5308.609 kb on + strand, within Pf1N1B4_4805at 5308.696 kb on + strand, within Pf1N1B4_4805at 5308.696 kb on + strand, within Pf1N1B4_4805at 5308.697 kb on - strand, within Pf1N1B4_4805at 5308.710 kb on + strandat 5308.711 kb on - strandat 5308.711 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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5,306,208 + +0.1
5,306,209 - +1.0
5,306,209 - -1.6
5,306,209 - +2.5
5,306,209 - +0.8
5,306,209 - +2.1
5,306,209 - -1.4
5,306,263 + +0.8
5,306,264 - +0.6
5,306,264 - +0.1
5,306,264 - -0.3
5,306,264 - -1.9
5,306,264 - +0.2
5,306,264 - -0.3
5,306,455 + Pf1N1B4_4803 0.25 +0.5
5,306,456 - Pf1N1B4_4803 0.25 +0.1
5,306,456 - Pf1N1B4_4803 0.25 +0.1
5,306,456 - Pf1N1B4_4803 0.25 -2.1
5,306,744 - Pf1N1B4_4803 0.61 -0.4
5,307,061 - +2.2
5,307,075 + +0.2
5,307,076 - -0.2
5,307,076 - -0.4
5,307,076 - +0.2
5,307,110 + +1.2
5,307,111 - -1.4
5,307,191 + -1.4
5,307,191 + -0.6
5,307,192 - -1.5
5,307,192 - -1.1
5,307,194 + +0.3
5,307,194 + +0.3
5,307,868 - -0.1
5,307,868 - -0.4
5,307,868 - +0.7
5,307,868 - +1.1
5,308,045 + Pf1N1B4_4805 0.15 +0.3
5,308,048 + Pf1N1B4_4805 0.15 -0.2
5,308,049 - -1.4
5,308,049 - Pf1N1B4_4805 0.15 +0.2
5,308,049 - +0.8
5,308,066 + Pf1N1B4_4805 0.17 +1.2
5,308,067 - Pf1N1B4_4805 0.17 -1.6
5,308,280 - Pf1N1B4_4805 0.42 -0.3
5,308,451 - Pf1N1B4_4805 0.61 -0.7
5,308,451 - Pf1N1B4_4805 0.61 -1.1
5,308,451 - Pf1N1B4_4805 0.61 -0.0
5,308,577 + Pf1N1B4_4805 0.75 +1.6
5,308,609 + Pf1N1B4_4805 0.79 -0.5
5,308,696 + Pf1N1B4_4805 0.89 -0.8
5,308,696 + Pf1N1B4_4805 0.89 +0.1
5,308,697 - Pf1N1B4_4805 0.89 +0.3
5,308,710 + -1.0
5,308,711 - +0.6
5,308,711 - +0.5

Or see this region's nucleotide sequence