Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4803 and Pf1N1B4_4804 are separated by 92 nucleotides Pf1N1B4_4804 and Pf1N1B4_4805 are separated by 10 nucleotides
Pf1N1B4_4803 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 5,306,262 to 5,307,047
_4803
Pf1N1B4_4804 - ABC-type polar amino acid transport system protein, ATP-binding protein, at 5,307,140 to 5,307,904
_4804
Pf1N1B4_4805 - ABC transporter permease protein, at 5,307,915 to 5,308,793
_4805
Position (kb)
5307
5308 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5306.208 kb on + strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.263 kb on + strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.455 kb on + strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.744 kb on - strand, within Pf1N1B4_4803 at 5307.061 kb on - strand at 5307.075 kb on + strand at 5307.076 kb on - strand at 5307.076 kb on - strand at 5307.076 kb on - strand at 5307.110 kb on + strand at 5307.111 kb on - strand at 5307.191 kb on + strand at 5307.191 kb on + strand at 5307.192 kb on - strand at 5307.192 kb on - strand at 5307.194 kb on + strand at 5307.194 kb on + strand at 5307.868 kb on - strand at 5307.868 kb on - strand at 5307.868 kb on - strand at 5307.868 kb on - strand at 5308.045 kb on + strand, within Pf1N1B4_4805 at 5308.048 kb on + strand, within Pf1N1B4_4805 at 5308.049 kb on - strand at 5308.049 kb on - strand, within Pf1N1B4_4805 at 5308.049 kb on - strand at 5308.066 kb on + strand, within Pf1N1B4_4805 at 5308.067 kb on - strand, within Pf1N1B4_4805 at 5308.280 kb on - strand, within Pf1N1B4_4805 at 5308.451 kb on - strand, within Pf1N1B4_4805 at 5308.451 kb on - strand, within Pf1N1B4_4805 at 5308.451 kb on - strand, within Pf1N1B4_4805 at 5308.577 kb on + strand, within Pf1N1B4_4805 at 5308.609 kb on + strand, within Pf1N1B4_4805 at 5308.696 kb on + strand, within Pf1N1B4_4805 at 5308.696 kb on + strand, within Pf1N1B4_4805 at 5308.697 kb on - strand, within Pf1N1B4_4805 at 5308.710 kb on + strand at 5308.711 kb on - strand at 5308.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,306,208 + +0.1 5,306,209 - +1.0 5,306,209 - -1.6 5,306,209 - +2.5 5,306,209 - +0.8 5,306,209 - +2.1 5,306,209 - -1.4 5,306,263 + +0.8 5,306,264 - +0.6 5,306,264 - +0.1 5,306,264 - -0.3 5,306,264 - -1.9 5,306,264 - +0.2 5,306,264 - -0.3 5,306,455 + Pf1N1B4_4803 0.25 +0.5 5,306,456 - Pf1N1B4_4803 0.25 +0.1 5,306,456 - Pf1N1B4_4803 0.25 +0.1 5,306,456 - Pf1N1B4_4803 0.25 -2.1 5,306,744 - Pf1N1B4_4803 0.61 -0.4 5,307,061 - +2.2 5,307,075 + +0.2 5,307,076 - -0.2 5,307,076 - -0.4 5,307,076 - +0.2 5,307,110 + +1.2 5,307,111 - -1.4 5,307,191 + -1.4 5,307,191 + -0.6 5,307,192 - -1.5 5,307,192 - -1.1 5,307,194 + +0.3 5,307,194 + +0.3 5,307,868 - -0.1 5,307,868 - -0.4 5,307,868 - +0.7 5,307,868 - +1.1 5,308,045 + Pf1N1B4_4805 0.15 +0.3 5,308,048 + Pf1N1B4_4805 0.15 -0.2 5,308,049 - -1.4 5,308,049 - Pf1N1B4_4805 0.15 +0.2 5,308,049 - +0.8 5,308,066 + Pf1N1B4_4805 0.17 +1.2 5,308,067 - Pf1N1B4_4805 0.17 -1.6 5,308,280 - Pf1N1B4_4805 0.42 -0.3 5,308,451 - Pf1N1B4_4805 0.61 -0.7 5,308,451 - Pf1N1B4_4805 0.61 -1.1 5,308,451 - Pf1N1B4_4805 0.61 -0.0 5,308,577 + Pf1N1B4_4805 0.75 +1.6 5,308,609 + Pf1N1B4_4805 0.79 -0.5 5,308,696 + Pf1N1B4_4805 0.89 -0.8 5,308,696 + Pf1N1B4_4805 0.89 +0.1 5,308,697 - Pf1N1B4_4805 0.89 +0.3 5,308,710 + -1.0 5,308,711 - +0.6 5,308,711 - +0.5
Or see this region's nucleotide sequence