Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4650 and Pf1N1B4_4651 are separated by 92 nucleotides Pf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotides Pf1N1B4_4652 and Pf1N1B4_4653 are separated by 126 nucleotides Pf1N1B4_4653 and Pf1N1B4_4654 are separated by 15 nucleotides
Pf1N1B4_4650 - Aspartate racemase (EC 5.1.1.13), at 5,129,551 to 5,130,243
_4650
Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112
_4651
Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677
_4652
Pf1N1B4_4653 - FIG00960119: hypothetical protein, at 5,131,804 to 5,132,025
_4653
Pf1N1B4_4654 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 5,132,041 to 5,133,507
_4654
Position (kb)
5131
5132 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5130.192 kb on - strand at 5130.192 kb on - strand at 5130.257 kb on - strand at 5130.321 kb on - strand at 5130.406 kb on - strand at 5130.528 kb on + strand at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand at 5130.607 kb on + strand, within Pf1N1B4_4651 at 5130.623 kb on - strand, within Pf1N1B4_4651 at 5130.689 kb on - strand, within Pf1N1B4_4651 at 5131.111 kb on - strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.370 kb on - strand, within Pf1N1B4_4652 at 5132.003 kb on - strand at 5132.109 kb on - strand at 5132.282 kb on + strand, within Pf1N1B4_4654 at 5132.379 kb on - strand, within Pf1N1B4_4654 at 5132.454 kb on - strand, within Pf1N1B4_4654 at 5132.516 kb on + strand, within Pf1N1B4_4654 at 5132.569 kb on - strand, within Pf1N1B4_4654
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,130,192 - +0.7 5,130,192 - -0.6 5,130,257 - -1.4 5,130,321 - -0.8 5,130,406 - +0.4 5,130,528 + -0.4 5,130,530 + Pf1N1B4_4651 0.25 +0.5 5,130,530 + Pf1N1B4_4651 0.25 -0.3 5,130,531 - Pf1N1B4_4651 0.25 +0.9 5,130,531 - Pf1N1B4_4651 0.25 -1.5 5,130,531 - Pf1N1B4_4651 0.25 +0.6 5,130,531 - -0.2 5,130,607 + Pf1N1B4_4651 0.35 +0.0 5,130,623 - Pf1N1B4_4651 0.37 -0.8 5,130,689 - Pf1N1B4_4651 0.45 +0.3 5,131,111 - -1.2 5,131,222 + +0.1 5,131,222 + -0.3 5,131,222 + +2.1 5,131,370 - Pf1N1B4_4652 0.38 +1.1 5,132,003 - -0.4 5,132,109 - -1.7 5,132,282 + Pf1N1B4_4654 0.16 -2.8 5,132,379 - Pf1N1B4_4654 0.23 -1.7 5,132,454 - Pf1N1B4_4654 0.28 -1.2 5,132,516 + Pf1N1B4_4654 0.32 -0.2 5,132,569 - Pf1N1B4_4654 0.36 -1.6
Or see this region's nucleotide sequence