Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4537

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4535 and Pf1N1B4_4536 are separated by 15 nucleotidesPf1N1B4_4536 and Pf1N1B4_4537 are separated by 74 nucleotidesPf1N1B4_4537 and Pf1N1B4_4538 are separated by 28 nucleotidesPf1N1B4_4538 and Pf1N1B4_4539 are separated by 228 nucleotides Pf1N1B4_4535 - Uncharacterized protein ImpF, at 5,003,241 to 5,003,684 _4535 Pf1N1B4_4536 - Uncharacterized protein ImpD, at 5,003,700 to 5,004,197 _4536 Pf1N1B4_4537 - Uncharacterized protein ImpC, at 5,004,272 to 5,005,759 _4537 Pf1N1B4_4538 - Uncharacterized protein ImpB, at 5,005,788 to 5,006,336 _4538 Pf1N1B4_4539 - Uncharacterized protein homologous to Paeru. PA2364, at 5,006,565 to 5,007,014 _4539 Position (kb) 5004 5005 5006Strain fitness (log2 ratio) -1 0 1 2at 5003.667 kb on + strandat 5004.056 kb on + strand, within Pf1N1B4_4536at 5004.226 kb on + strandat 5004.230 kb on + strandat 5004.230 kb on + strandat 5004.230 kb on + strandat 5004.231 kb on - strandat 5004.369 kb on + strandat 5004.589 kb on - strandat 5004.589 kb on - strand, within Pf1N1B4_4537at 5004.639 kb on + strand, within Pf1N1B4_4537at 5004.640 kb on - strand, within Pf1N1B4_4537at 5004.812 kb on - strand, within Pf1N1B4_4537at 5004.936 kb on + strandat 5004.937 kb on - strand, within Pf1N1B4_4537at 5004.937 kb on - strand, within Pf1N1B4_4537at 5004.973 kb on - strand, within Pf1N1B4_4537at 5005.324 kb on - strand, within Pf1N1B4_4537at 5005.641 kb on + strandat 5005.642 kb on - strandat 5005.720 kb on + strandat 5006.275 kb on + strand, within Pf1N1B4_4538at 5006.403 kb on - strandat 5006.511 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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5,003,667 + +0.6
5,004,056 + Pf1N1B4_4536 0.71 +0.4
5,004,226 + +0.8
5,004,230 + +0.3
5,004,230 + -1.4
5,004,230 + +1.6
5,004,231 - -0.5
5,004,369 + -0.2
5,004,589 - +0.6
5,004,589 - Pf1N1B4_4537 0.21 -1.2
5,004,639 + Pf1N1B4_4537 0.25 -0.2
5,004,640 - Pf1N1B4_4537 0.25 -1.0
5,004,812 - Pf1N1B4_4537 0.36 -0.9
5,004,936 + +1.6
5,004,937 - Pf1N1B4_4537 0.45 -1.1
5,004,937 - Pf1N1B4_4537 0.45 -0.1
5,004,973 - Pf1N1B4_4537 0.47 -1.7
5,005,324 - Pf1N1B4_4537 0.71 +1.0
5,005,641 + -0.4
5,005,642 - -0.5
5,005,720 + +0.0
5,006,275 + Pf1N1B4_4538 0.89 +0.4
5,006,403 - -0.2
5,006,511 - +1.9

Or see this region's nucleotide sequence