Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4535 and Pf1N1B4_4536 are separated by 15 nucleotides Pf1N1B4_4536 and Pf1N1B4_4537 are separated by 74 nucleotides Pf1N1B4_4537 and Pf1N1B4_4538 are separated by 28 nucleotides Pf1N1B4_4538 and Pf1N1B4_4539 are separated by 228 nucleotides
Pf1N1B4_4535 - Uncharacterized protein ImpF, at 5,003,241 to 5,003,684
_4535
Pf1N1B4_4536 - Uncharacterized protein ImpD, at 5,003,700 to 5,004,197
_4536
Pf1N1B4_4537 - Uncharacterized protein ImpC, at 5,004,272 to 5,005,759
_4537
Pf1N1B4_4538 - Uncharacterized protein ImpB, at 5,005,788 to 5,006,336
_4538
Pf1N1B4_4539 - Uncharacterized protein homologous to Paeru. PA2364, at 5,006,565 to 5,007,014
_4539
Position (kb)
5004
5005
5006 Strain fitness (log2 ratio)
-1
0
1
2 at 5003.667 kb on + strand at 5004.056 kb on + strand, within Pf1N1B4_4536 at 5004.226 kb on + strand at 5004.230 kb on + strand at 5004.230 kb on + strand at 5004.230 kb on + strand at 5004.231 kb on - strand at 5004.369 kb on + strand at 5004.589 kb on - strand at 5004.589 kb on - strand, within Pf1N1B4_4537 at 5004.639 kb on + strand, within Pf1N1B4_4537 at 5004.640 kb on - strand, within Pf1N1B4_4537 at 5004.812 kb on - strand, within Pf1N1B4_4537 at 5004.936 kb on + strand at 5004.937 kb on - strand, within Pf1N1B4_4537 at 5004.937 kb on - strand, within Pf1N1B4_4537 at 5004.973 kb on - strand, within Pf1N1B4_4537 at 5005.324 kb on - strand, within Pf1N1B4_4537 at 5005.641 kb on + strand at 5005.642 kb on - strand at 5005.720 kb on + strand at 5006.275 kb on + strand, within Pf1N1B4_4538 at 5006.403 kb on - strand at 5006.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,003,667 + +0.6 5,004,056 + Pf1N1B4_4536 0.71 +0.4 5,004,226 + +0.8 5,004,230 + +0.3 5,004,230 + -1.4 5,004,230 + +1.6 5,004,231 - -0.5 5,004,369 + -0.2 5,004,589 - +0.6 5,004,589 - Pf1N1B4_4537 0.21 -1.2 5,004,639 + Pf1N1B4_4537 0.25 -0.2 5,004,640 - Pf1N1B4_4537 0.25 -1.0 5,004,812 - Pf1N1B4_4537 0.36 -0.9 5,004,936 + +1.6 5,004,937 - Pf1N1B4_4537 0.45 -1.1 5,004,937 - Pf1N1B4_4537 0.45 -0.1 5,004,973 - Pf1N1B4_4537 0.47 -1.7 5,005,324 - Pf1N1B4_4537 0.71 +1.0 5,005,641 + -0.4 5,005,642 - -0.5 5,005,720 + +0.0 5,006,275 + Pf1N1B4_4538 0.89 +0.4 5,006,403 - -0.2 5,006,511 - +1.9
Or see this region's nucleotide sequence