Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_452

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_450 and Pf1N1B4_451 are separated by 191 nucleotidesPf1N1B4_451 and Pf1N1B4_452 are separated by 16 nucleotidesPf1N1B4_452 and Pf1N1B4_453 are separated by 178 nucleotides Pf1N1B4_450 - Acyl carrier protein, at 542,382 to 542,618 _450 Pf1N1B4_451 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 542,810 to 543,553 _451 Pf1N1B4_452 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 543,570 to 544,454 _452 Pf1N1B4_453 - Phosphate:acyl-ACP acyltransferase PlsX, at 544,633 to 545,613 _453 Position (kb) 543 544 545Strain fitness (log2 ratio) -1 0 1 2at 542.668 kb on - strandat 542.676 kb on - strandat 542.812 kb on - strandat 544.522 kb on - strandat 544.611 kb on - strandat 544.611 kb on - strandat 544.611 kb on - strandat 544.611 kb on - strandat 545.018 kb on + strand, within Pf1N1B4_453at 545.018 kb on + strandat 545.019 kb on - strand, within Pf1N1B4_453

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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542,668 - +2.1
542,676 - +2.1
542,812 - +1.5
544,522 - -0.6
544,611 - +0.1
544,611 - -0.2
544,611 - -1.3
544,611 - -0.8
545,018 + Pf1N1B4_453 0.39 -0.8
545,018 + -0.6
545,019 - Pf1N1B4_453 0.39 -0.5

Or see this region's nucleotide sequence