Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4504

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4503 and Pf1N1B4_4504 are separated by 3 nucleotidesPf1N1B4_4504 and Pf1N1B4_4505 overlap by 23 nucleotides Pf1N1B4_4503 - Coenzyme PQQ synthesis protein B, at 4,965,547 to 4,966,461 _4503 Pf1N1B4_4504 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 4,966,465 to 4,966,872 _4504 Pf1N1B4_4505 - Coenzyme PQQ synthesis protein E, at 4,966,850 to 4,968,487 _4505 Position (kb) 4966 4967Strain fitness (log2 ratio) -1 0 1at 4965.475 kb on + strandat 4965.475 kb on + strandat 4965.476 kb on - strandat 4965.476 kb on - strandat 4966.359 kb on + strand, within Pf1N1B4_4503at 4966.436 kb on - strandat 4966.449 kb on + strandat 4966.522 kb on + strandat 4966.700 kb on - strand, within Pf1N1B4_4504at 4966.754 kb on - strand, within Pf1N1B4_4504at 4967.118 kb on - strand, within Pf1N1B4_4505at 4967.124 kb on - strand, within Pf1N1B4_4505at 4967.192 kb on + strand, within Pf1N1B4_4505at 4967.435 kb on + strand, within Pf1N1B4_4505at 4967.732 kb on - strand, within Pf1N1B4_4505

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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4,965,475 + +1.2
4,965,475 + +1.6
4,965,476 - -0.7
4,965,476 - +0.2
4,966,359 + Pf1N1B4_4503 0.89 +0.0
4,966,436 - -0.2
4,966,449 + -1.5
4,966,522 + +0.6
4,966,700 - Pf1N1B4_4504 0.58 -0.2
4,966,754 - Pf1N1B4_4504 0.71 -0.1
4,967,118 - Pf1N1B4_4505 0.16 -1.2
4,967,124 - Pf1N1B4_4505 0.17 -0.7
4,967,192 + Pf1N1B4_4505 0.21 +0.7
4,967,435 + Pf1N1B4_4505 0.36 +1.4
4,967,732 - Pf1N1B4_4505 0.54 -0.2

Or see this region's nucleotide sequence