Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4216

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4215 and Pf1N1B4_4216 are separated by 258 nucleotidesPf1N1B4_4216 and Pf1N1B4_4217 are separated by 43 nucleotidesPf1N1B4_4217 and Pf1N1B4_4218 are separated by 98 nucleotides Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435 _4215 Pf1N1B4_4216 - Putative secreted protein, at 4,671,694 to 4,672,446 _4216 Pf1N1B4_4217 - MISCELLANEOUS; Hypothetical/Global homology, at 4,672,490 to 4,672,903 _4217 Pf1N1B4_4218 - Multidrug resistance protein A, at 4,673,002 to 4,674,099 _4218 Position (kb) 4671 4672 4673Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4670.751 kb on + strand, within Pf1N1B4_4215at 4670.751 kb on + strand, within Pf1N1B4_4215at 4670.751 kb on + strand, within Pf1N1B4_4215at 4670.752 kb on - strand, within Pf1N1B4_4215at 4670.752 kb on - strand, within Pf1N1B4_4215at 4670.752 kb on - strandat 4670.794 kb on + strand, within Pf1N1B4_4215at 4670.820 kb on + strand, within Pf1N1B4_4215at 4670.886 kb on + strand, within Pf1N1B4_4215at 4670.888 kb on + strand, within Pf1N1B4_4215at 4670.888 kb on + strand, within Pf1N1B4_4215at 4670.888 kb on + strand, within Pf1N1B4_4215at 4670.888 kb on + strand, within Pf1N1B4_4215at 4670.889 kb on - strand, within Pf1N1B4_4215at 4670.889 kb on - strand, within Pf1N1B4_4215at 4670.889 kb on - strand, within Pf1N1B4_4215at 4670.979 kb on - strand, within Pf1N1B4_4215at 4671.337 kb on + strandat 4671.337 kb on + strandat 4671.338 kb on - strandat 4671.413 kb on + strandat 4671.413 kb on + strandat 4671.413 kb on + strandat 4671.414 kb on - strandat 4671.414 kb on - strandat 4671.804 kb on + strand, within Pf1N1B4_4216at 4671.804 kb on + strandat 4672.067 kb on - strand, within Pf1N1B4_4216at 4672.469 kb on + strandat 4672.469 kb on + strandat 4672.470 kb on - strandat 4672.470 kb on - strandat 4672.644 kb on - strand, within Pf1N1B4_4217at 4672.697 kb on + strand, within Pf1N1B4_4217at 4672.697 kb on + strand, within Pf1N1B4_4217at 4672.697 kb on + strand, within Pf1N1B4_4217at 4672.698 kb on - strand, within Pf1N1B4_4217at 4672.698 kb on - strand, within Pf1N1B4_4217at 4672.698 kb on - strand, within Pf1N1B4_4217at 4672.698 kb on - strand, within Pf1N1B4_4217at 4672.949 kb on + strandat 4672.950 kb on - strandat 4672.950 kb on - strandat 4672.950 kb on - strandat 4673.185 kb on + strand, within Pf1N1B4_4218at 4673.185 kb on + strand, within Pf1N1B4_4218at 4673.185 kb on + strandat 4673.185 kb on + strand, within Pf1N1B4_4218at 4673.185 kb on + strand, within Pf1N1B4_4218at 4673.186 kb on - strand, within Pf1N1B4_4218at 4673.230 kb on + strand, within Pf1N1B4_4218at 4673.231 kb on - strand, within Pf1N1B4_4218

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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4,670,751 + Pf1N1B4_4215 0.57 -0.5
4,670,751 + Pf1N1B4_4215 0.57 -0.6
4,670,751 + Pf1N1B4_4215 0.57 +1.6
4,670,752 - Pf1N1B4_4215 0.57 -0.1
4,670,752 - Pf1N1B4_4215 0.57 -0.3
4,670,752 - -0.4
4,670,794 + Pf1N1B4_4215 0.60 +1.6
4,670,820 + Pf1N1B4_4215 0.61 -0.7
4,670,886 + Pf1N1B4_4215 0.65 +0.4
4,670,888 + Pf1N1B4_4215 0.66 -0.6
4,670,888 + Pf1N1B4_4215 0.66 -1.6
4,670,888 + Pf1N1B4_4215 0.66 +0.0
4,670,888 + Pf1N1B4_4215 0.66 -1.5
4,670,889 - Pf1N1B4_4215 0.66 +0.7
4,670,889 - Pf1N1B4_4215 0.66 +1.0
4,670,889 - Pf1N1B4_4215 0.66 +0.5
4,670,979 - Pf1N1B4_4215 0.71 -2.1
4,671,337 + +1.1
4,671,337 + +0.0
4,671,338 - -0.5
4,671,413 + +1.3
4,671,413 + -0.5
4,671,413 + +0.5
4,671,414 - -0.7
4,671,414 - -0.4
4,671,804 + Pf1N1B4_4216 0.15 -0.4
4,671,804 + -1.0
4,672,067 - Pf1N1B4_4216 0.50 +0.7
4,672,469 + -1.5
4,672,469 + -0.9
4,672,470 - +0.8
4,672,470 - -0.2
4,672,644 - Pf1N1B4_4217 0.37 -0.6
4,672,697 + Pf1N1B4_4217 0.50 -0.8
4,672,697 + Pf1N1B4_4217 0.50 -0.3
4,672,697 + Pf1N1B4_4217 0.50 -1.0
4,672,698 - Pf1N1B4_4217 0.50 -2.1
4,672,698 - Pf1N1B4_4217 0.50 -1.1
4,672,698 - Pf1N1B4_4217 0.50 +0.9
4,672,698 - Pf1N1B4_4217 0.50 +0.7
4,672,949 + -0.6
4,672,950 - -1.9
4,672,950 - +0.2
4,672,950 - +2.7
4,673,185 + Pf1N1B4_4218 0.17 +0.9
4,673,185 + Pf1N1B4_4218 0.17 +0.2
4,673,185 + -1.0
4,673,185 + Pf1N1B4_4218 0.17 -0.4
4,673,185 + Pf1N1B4_4218 0.17 +0.6
4,673,186 - Pf1N1B4_4218 0.17 -1.4
4,673,230 + Pf1N1B4_4218 0.21 +0.0
4,673,231 - Pf1N1B4_4218 0.21 -3.1

Or see this region's nucleotide sequence