Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4215 and Pf1N1B4_4216 are separated by 258 nucleotides Pf1N1B4_4216 and Pf1N1B4_4217 are separated by 43 nucleotides Pf1N1B4_4217 and Pf1N1B4_4218 are separated by 98 nucleotides
Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435
_4215
Pf1N1B4_4216 - Putative secreted protein, at 4,671,694 to 4,672,446
_4216
Pf1N1B4_4217 - MISCELLANEOUS; Hypothetical/Global homology, at 4,672,490 to 4,672,903
_4217
Pf1N1B4_4218 - Multidrug resistance protein A, at 4,673,002 to 4,674,099
_4218
Position (kb)
4671
4672
4673 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.751 kb on + strand, within Pf1N1B4_4215 at 4670.752 kb on - strand, within Pf1N1B4_4215 at 4670.752 kb on - strand, within Pf1N1B4_4215 at 4670.752 kb on - strand at 4670.794 kb on + strand, within Pf1N1B4_4215 at 4670.820 kb on + strand, within Pf1N1B4_4215 at 4670.886 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.888 kb on + strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.889 kb on - strand, within Pf1N1B4_4215 at 4670.979 kb on - strand, within Pf1N1B4_4215 at 4671.337 kb on + strand at 4671.337 kb on + strand at 4671.338 kb on - strand at 4671.413 kb on + strand at 4671.413 kb on + strand at 4671.413 kb on + strand at 4671.414 kb on - strand at 4671.414 kb on - strand at 4671.804 kb on + strand, within Pf1N1B4_4216 at 4671.804 kb on + strand at 4672.067 kb on - strand, within Pf1N1B4_4216 at 4672.469 kb on + strand at 4672.469 kb on + strand at 4672.470 kb on - strand at 4672.470 kb on - strand at 4672.644 kb on - strand, within Pf1N1B4_4217 at 4672.697 kb on + strand, within Pf1N1B4_4217 at 4672.697 kb on + strand, within Pf1N1B4_4217 at 4672.697 kb on + strand, within Pf1N1B4_4217 at 4672.698 kb on - strand, within Pf1N1B4_4217 at 4672.698 kb on - strand, within Pf1N1B4_4217 at 4672.698 kb on - strand, within Pf1N1B4_4217 at 4672.698 kb on - strand, within Pf1N1B4_4217 at 4672.949 kb on + strand at 4672.950 kb on - strand at 4672.950 kb on - strand at 4672.950 kb on - strand at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.186 kb on - strand, within Pf1N1B4_4218 at 4673.230 kb on + strand, within Pf1N1B4_4218 at 4673.231 kb on - strand, within Pf1N1B4_4218
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,670,751 + Pf1N1B4_4215 0.57 -0.5 4,670,751 + Pf1N1B4_4215 0.57 -0.6 4,670,751 + Pf1N1B4_4215 0.57 +1.6 4,670,752 - Pf1N1B4_4215 0.57 -0.1 4,670,752 - Pf1N1B4_4215 0.57 -0.3 4,670,752 - -0.4 4,670,794 + Pf1N1B4_4215 0.60 +1.6 4,670,820 + Pf1N1B4_4215 0.61 -0.7 4,670,886 + Pf1N1B4_4215 0.65 +0.4 4,670,888 + Pf1N1B4_4215 0.66 -0.6 4,670,888 + Pf1N1B4_4215 0.66 -1.6 4,670,888 + Pf1N1B4_4215 0.66 +0.0 4,670,888 + Pf1N1B4_4215 0.66 -1.5 4,670,889 - Pf1N1B4_4215 0.66 +0.7 4,670,889 - Pf1N1B4_4215 0.66 +1.0 4,670,889 - Pf1N1B4_4215 0.66 +0.5 4,670,979 - Pf1N1B4_4215 0.71 -2.1 4,671,337 + +1.1 4,671,337 + +0.0 4,671,338 - -0.5 4,671,413 + +1.3 4,671,413 + -0.5 4,671,413 + +0.5 4,671,414 - -0.7 4,671,414 - -0.4 4,671,804 + Pf1N1B4_4216 0.15 -0.4 4,671,804 + -1.0 4,672,067 - Pf1N1B4_4216 0.50 +0.7 4,672,469 + -1.5 4,672,469 + -0.9 4,672,470 - +0.8 4,672,470 - -0.2 4,672,644 - Pf1N1B4_4217 0.37 -0.6 4,672,697 + Pf1N1B4_4217 0.50 -0.8 4,672,697 + Pf1N1B4_4217 0.50 -0.3 4,672,697 + Pf1N1B4_4217 0.50 -1.0 4,672,698 - Pf1N1B4_4217 0.50 -2.1 4,672,698 - Pf1N1B4_4217 0.50 -1.1 4,672,698 - Pf1N1B4_4217 0.50 +0.9 4,672,698 - Pf1N1B4_4217 0.50 +0.7 4,672,949 + -0.6 4,672,950 - -1.9 4,672,950 - +0.2 4,672,950 - +2.7 4,673,185 + Pf1N1B4_4218 0.17 +0.9 4,673,185 + Pf1N1B4_4218 0.17 +0.2 4,673,185 + -1.0 4,673,185 + Pf1N1B4_4218 0.17 -0.4 4,673,185 + Pf1N1B4_4218 0.17 +0.6 4,673,186 - Pf1N1B4_4218 0.17 -1.4 4,673,230 + Pf1N1B4_4218 0.21 +0.0 4,673,231 - Pf1N1B4_4218 0.21 -3.1
Or see this region's nucleotide sequence