Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4077

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4075 and Pf1N1B4_4076 are separated by 113 nucleotidesPf1N1B4_4076 and Pf1N1B4_4077 are separated by 147 nucleotidesPf1N1B4_4077 and Pf1N1B4_4078 are separated by 100 nucleotides Pf1N1B4_4075 - hypothetical protein, at 4,526,003 to 4,526,353 _4075 Pf1N1B4_4076 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,526,467 to 4,526,895 _4076 Pf1N1B4_4077 - FIG00962160: hypothetical protein, at 4,527,043 to 4,527,480 _4077 Pf1N1B4_4078 - GDP-mannose pyrophosphatase YffH, at 4,527,581 to 4,528,174 _4078 Position (kb) 4527 4528Strain fitness (log2 ratio) -2 -1 0 1 2at 4526.090 kb on + strand, within Pf1N1B4_4075at 4526.189 kb on + strand, within Pf1N1B4_4075at 4526.190 kb on - strand, within Pf1N1B4_4075at 4526.190 kb on - strand, within Pf1N1B4_4075at 4526.190 kb on - strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strand, within Pf1N1B4_4075at 4526.225 kb on + strandat 4526.226 kb on - strand, within Pf1N1B4_4075at 4526.251 kb on - strand, within Pf1N1B4_4075at 4526.288 kb on + strand, within Pf1N1B4_4075at 4526.288 kb on + strand, within Pf1N1B4_4075at 4526.288 kb on + strand, within Pf1N1B4_4075at 4526.288 kb on + strandat 4526.289 kb on - strand, within Pf1N1B4_4075at 4526.291 kb on + strand, within Pf1N1B4_4075at 4526.352 kb on - strandat 4526.352 kb on - strandat 4526.352 kb on - strandat 4526.352 kb on - strandat 4526.352 kb on - strandat 4526.352 kb on - strandat 4526.411 kb on + strandat 4526.411 kb on + strandat 4526.412 kb on - strandat 4526.412 kb on - strandat 4526.412 kb on - strandat 4526.412 kb on - strandat 4526.464 kb on - strandat 4526.464 kb on - strandat 4526.464 kb on - strandat 4526.469 kb on - strandat 4526.469 kb on - strandat 4526.536 kb on + strand, within Pf1N1B4_4076at 4526.562 kb on - strand, within Pf1N1B4_4076at 4526.718 kb on - strand, within Pf1N1B4_4076at 4526.733 kb on - strand, within Pf1N1B4_4076at 4526.818 kb on - strand, within Pf1N1B4_4076at 4526.820 kb on - strandat 4526.820 kb on - strand, within Pf1N1B4_4076at 4526.820 kb on - strand, within Pf1N1B4_4076at 4526.820 kb on - strand, within Pf1N1B4_4076at 4526.895 kb on + strandat 4526.896 kb on - strandat 4526.896 kb on - strandat 4526.900 kb on + strandat 4526.901 kb on - strandat 4526.901 kb on - strandat 4526.998 kb on - strandat 4527.145 kb on + strand, within Pf1N1B4_4077at 4527.146 kb on - strand, within Pf1N1B4_4077at 4527.202 kb on + strand, within Pf1N1B4_4077at 4527.203 kb on - strandat 4527.241 kb on - strand, within Pf1N1B4_4077at 4527.448 kb on + strandat 4527.460 kb on + strandat 4527.461 kb on - strandat 4527.461 kb on - strandat 4527.565 kb on + strandat 4527.566 kb on - strandat 4527.641 kb on + strand, within Pf1N1B4_4078at 4527.641 kb on + strand, within Pf1N1B4_4078at 4527.669 kb on - strand, within Pf1N1B4_4078at 4527.713 kb on + strand, within Pf1N1B4_4078at 4527.941 kb on + strand, within Pf1N1B4_4078at 4527.942 kb on - strand, within Pf1N1B4_4078at 4527.942 kb on - strand, within Pf1N1B4_4078at 4527.942 kb on - strand, within Pf1N1B4_4078at 4528.094 kb on + strandat 4528.094 kb on + strand, within Pf1N1B4_4078at 4528.095 kb on - strand, within Pf1N1B4_4078at 4528.139 kb on + strandat 4528.314 kb on - strandat 4528.314 kb on - strandat 4528.451 kb on + strandat 4528.451 kb on + strandat 4528.452 kb on - strandat 4528.452 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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4,526,090 + Pf1N1B4_4075 0.25 -1.2
4,526,189 + Pf1N1B4_4075 0.53 -0.7
4,526,190 - Pf1N1B4_4075 0.53 -0.7
4,526,190 - Pf1N1B4_4075 0.53 -0.4
4,526,190 - Pf1N1B4_4075 0.53 -0.3
4,526,225 + Pf1N1B4_4075 0.63 +0.8
4,526,225 + Pf1N1B4_4075 0.63 -1.4
4,526,225 + Pf1N1B4_4075 0.63 +1.1
4,526,225 + Pf1N1B4_4075 0.63 -0.4
4,526,225 + Pf1N1B4_4075 0.63 -0.7
4,526,225 + Pf1N1B4_4075 0.63 +2.0
4,526,225 + +2.6
4,526,226 - Pf1N1B4_4075 0.64 -0.1
4,526,251 - Pf1N1B4_4075 0.71 -2.0
4,526,288 + Pf1N1B4_4075 0.81 -1.4
4,526,288 + Pf1N1B4_4075 0.81 +0.1
4,526,288 + Pf1N1B4_4075 0.81 +0.7
4,526,288 + +0.6
4,526,289 - Pf1N1B4_4075 0.81 -1.6
4,526,291 + Pf1N1B4_4075 0.82 +0.1
4,526,352 - -0.5
4,526,352 - -1.6
4,526,352 - +0.7
4,526,352 - -0.3
4,526,352 - +1.4
4,526,352 - +0.2
4,526,411 + -0.6
4,526,411 + -0.4
4,526,412 - +1.6
4,526,412 - +0.4
4,526,412 - -0.3
4,526,412 - +1.0
4,526,464 - +0.7
4,526,464 - -0.1
4,526,464 - -2.1
4,526,469 - -1.3
4,526,469 - +1.0
4,526,536 + Pf1N1B4_4076 0.16 -0.9
4,526,562 - Pf1N1B4_4076 0.22 +0.6
4,526,718 - Pf1N1B4_4076 0.59 -0.4
4,526,733 - Pf1N1B4_4076 0.62 +0.0
4,526,818 - Pf1N1B4_4076 0.82 +0.3
4,526,820 - -1.6
4,526,820 - Pf1N1B4_4076 0.82 +0.8
4,526,820 - Pf1N1B4_4076 0.82 -0.7
4,526,820 - Pf1N1B4_4076 0.82 +0.0
4,526,895 + +0.5
4,526,896 - +0.6
4,526,896 - +0.9
4,526,900 + -0.4
4,526,901 - -0.2
4,526,901 - +1.4
4,526,998 - +0.3
4,527,145 + Pf1N1B4_4077 0.23 -1.9
4,527,146 - Pf1N1B4_4077 0.24 -0.4
4,527,202 + Pf1N1B4_4077 0.36 -0.6
4,527,203 - -0.2
4,527,241 - Pf1N1B4_4077 0.45 -0.4
4,527,448 + -0.5
4,527,460 + -1.7
4,527,461 - -0.7
4,527,461 - -0.3
4,527,565 + -0.3
4,527,566 - -0.3
4,527,641 + Pf1N1B4_4078 0.10 +0.5
4,527,641 + Pf1N1B4_4078 0.10 +0.2
4,527,669 - Pf1N1B4_4078 0.15 +0.6
4,527,713 + Pf1N1B4_4078 0.22 +0.5
4,527,941 + Pf1N1B4_4078 0.61 +0.3
4,527,942 - Pf1N1B4_4078 0.61 -1.3
4,527,942 - Pf1N1B4_4078 0.61 +0.1
4,527,942 - Pf1N1B4_4078 0.61 +0.6
4,528,094 + -0.4
4,528,094 + Pf1N1B4_4078 0.86 -0.8
4,528,095 - Pf1N1B4_4078 0.87 +0.7
4,528,139 + +0.3
4,528,314 - -0.5
4,528,314 - +1.6
4,528,451 + -1.7
4,528,451 + +0.3
4,528,452 - +1.0
4,528,452 - +1.1

Or see this region's nucleotide sequence