Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_401

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_400 and Pf1N1B4_401 are separated by 215 nucleotidesPf1N1B4_401 and Pf1N1B4_402 are separated by 220 nucleotides Pf1N1B4_400 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 469,384 to 470,376 _400 Pf1N1B4_401 - GCN5-related N-acetyltransferase (EC 2.3.1.57), at 470,592 to 471,074 _401 Pf1N1B4_402 - metal-dependent phosphohydrolase, at 471,295 to 471,951 _402 Position (kb) 470 471 472Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 469.629 kb on - strand, within Pf1N1B4_400at 470.324 kb on + strandat 470.324 kb on + strandat 470.324 kb on + strandat 470.324 kb on + strandat 470.325 kb on - strandat 470.332 kb on + strandat 470.332 kb on + strandat 470.333 kb on - strandat 470.418 kb on + strandat 470.419 kb on - strandat 470.420 kb on + strandat 470.421 kb on - strandat 470.436 kb on + strandat 470.447 kb on - strandat 470.448 kb on + strandat 470.450 kb on + strandat 470.523 kb on + strandat 470.523 kb on + strandat 470.523 kb on + strandat 470.523 kb on + strandat 470.523 kb on + strandat 470.558 kb on + strandat 470.559 kb on - strandat 470.559 kb on - strandat 470.680 kb on + strand, within Pf1N1B4_401at 470.681 kb on - strand, within Pf1N1B4_401at 470.681 kb on - strand, within Pf1N1B4_401at 470.815 kb on + strand, within Pf1N1B4_401at 470.815 kb on + strand, within Pf1N1B4_401at 470.815 kb on + strand, within Pf1N1B4_401at 470.815 kb on + strand, within Pf1N1B4_401at 470.815 kb on + strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.816 kb on - strand, within Pf1N1B4_401at 470.831 kb on - strand, within Pf1N1B4_401at 471.107 kb on - strandat 471.107 kb on - strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.109 kb on + strandat 471.110 kb on - strandat 471.110 kb on - strandat 471.110 kb on - strandat 471.110 kb on - strandat 471.155 kb on + strandat 471.155 kb on + strandat 471.155 kb on + strandat 471.155 kb on + strandat 471.155 kb on + strandat 471.155 kb on + strandat 471.156 kb on - strandat 471.156 kb on - strandat 471.156 kb on - strandat 471.156 kb on - strandat 471.161 kb on + strandat 471.161 kb on + strandat 471.181 kb on + strandat 471.181 kb on + strandat 471.181 kb on + strandat 471.182 kb on - strandat 471.217 kb on - strandat 471.363 kb on + strand, within Pf1N1B4_402at 471.364 kb on - strand, within Pf1N1B4_402at 471.564 kb on - strand, within Pf1N1B4_402at 471.591 kb on + strand, within Pf1N1B4_402at 471.724 kb on + strandat 471.724 kb on + strand, within Pf1N1B4_402at 471.724 kb on + strand, within Pf1N1B4_402at 471.724 kb on + strand, within Pf1N1B4_402at 471.724 kb on + strand, within Pf1N1B4_402at 471.725 kb on - strand, within Pf1N1B4_402at 471.725 kb on - strand, within Pf1N1B4_402at 471.725 kb on - strand, within Pf1N1B4_402at 471.725 kb on - strand, within Pf1N1B4_402at 471.725 kb on - strand, within Pf1N1B4_402at 471.754 kb on + strand, within Pf1N1B4_402at 471.754 kb on + strand, within Pf1N1B4_402at 471.754 kb on + strand, within Pf1N1B4_402at 471.755 kb on - strandat 471.755 kb on - strand, within Pf1N1B4_402at 471.755 kb on - strand, within Pf1N1B4_402at 471.755 kb on - strand, within Pf1N1B4_402at 471.790 kb on + strand, within Pf1N1B4_402at 471.949 kb on + strandat 471.949 kb on + strandat 472.025 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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469,629 - Pf1N1B4_400 0.25 -0.1
470,324 + +1.1
470,324 + +0.5
470,324 + +0.3
470,324 + -0.1
470,325 - -0.0
470,332 + +2.1
470,332 + -0.3
470,333 - +0.5
470,418 + -0.0
470,419 - -0.6
470,420 + -0.8
470,421 - +0.6
470,436 + -2.3
470,447 - -1.6
470,448 + -0.4
470,450 + -2.0
470,523 + +0.4
470,523 + -0.0
470,523 + -1.9
470,523 + +1.5
470,523 + -0.3
470,558 + -0.0
470,559 - -1.8
470,559 - -0.3
470,680 + Pf1N1B4_401 0.18 +0.2
470,681 - Pf1N1B4_401 0.18 -2.4
470,681 - Pf1N1B4_401 0.18 -0.5
470,815 + Pf1N1B4_401 0.46 -0.3
470,815 + Pf1N1B4_401 0.46 +0.1
470,815 + Pf1N1B4_401 0.46 -0.8
470,815 + Pf1N1B4_401 0.46 +0.5
470,815 + Pf1N1B4_401 0.46 +1.0
470,816 - Pf1N1B4_401 0.46 -1.0
470,816 - Pf1N1B4_401 0.46 +1.9
470,816 - Pf1N1B4_401 0.46 -1.3
470,816 - Pf1N1B4_401 0.46 -0.6
470,816 - Pf1N1B4_401 0.46 -0.5
470,816 - Pf1N1B4_401 0.46 -0.8
470,831 - Pf1N1B4_401 0.49 -0.9
471,107 - +1.3
471,107 - +0.4
471,109 + -1.3
471,109 + -0.4
471,109 + -0.6
471,109 + +0.5
471,109 + -0.8
471,109 + -1.7
471,109 + -0.2
471,109 + +1.5
471,109 + -0.3
471,109 + +0.1
471,110 - +1.5
471,110 - +1.1
471,110 - +0.3
471,110 - -0.8
471,155 + -0.1
471,155 + -0.2
471,155 + +2.0
471,155 + +0.5
471,155 + -1.1
471,155 + +2.3
471,156 - -0.2
471,156 - +0.2
471,156 - -1.8
471,156 - -0.8
471,161 + +0.6
471,161 + -0.5
471,181 + +0.1
471,181 + +1.0
471,181 + +1.1
471,182 - -0.5
471,217 - +0.5
471,363 + Pf1N1B4_402 0.10 -0.9
471,364 - Pf1N1B4_402 0.11 +0.5
471,564 - Pf1N1B4_402 0.41 -0.3
471,591 + Pf1N1B4_402 0.45 -1.3
471,724 + -0.0
471,724 + Pf1N1B4_402 0.65 -0.9
471,724 + Pf1N1B4_402 0.65 +0.6
471,724 + Pf1N1B4_402 0.65 +0.3
471,724 + Pf1N1B4_402 0.65 -0.0
471,725 - Pf1N1B4_402 0.65 +0.1
471,725 - Pf1N1B4_402 0.65 +0.4
471,725 - Pf1N1B4_402 0.65 -0.4
471,725 - Pf1N1B4_402 0.65 -1.0
471,725 - Pf1N1B4_402 0.65 -1.9
471,754 + Pf1N1B4_402 0.70 +0.0
471,754 + Pf1N1B4_402 0.70 +1.5
471,754 + Pf1N1B4_402 0.70 +0.8
471,755 - -1.5
471,755 - Pf1N1B4_402 0.70 -0.3
471,755 - Pf1N1B4_402 0.70 +0.5
471,755 - Pf1N1B4_402 0.70 -0.5
471,790 + Pf1N1B4_402 0.75 -3.5
471,949 + +0.3
471,949 + +1.7
472,025 + +0.0

Or see this region's nucleotide sequence