Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3779 and Pf1N1B4_3780 are separated by 143 nucleotides Pf1N1B4_3780 and Pf1N1B4_3781 are separated by 81 nucleotides
Pf1N1B4_3779 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,188,038 to 4,189,003
_3779
Pf1N1B4_3780 - Chorismate synthase (EC 4.2.3.5), at 4,189,147 to 4,190,238
_3780
Pf1N1B4_3781 - Nucleoside:H+ symporter:Major facilitator superfamily, at 4,190,320 to 4,191,465
_3781
Position (kb)
4189
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4188.476 kb on + strand, within Pf1N1B4_3779 at 4188.648 kb on + strand, within Pf1N1B4_3779 at 4188.648 kb on + strand, within Pf1N1B4_3779 at 4188.667 kb on + strand, within Pf1N1B4_3779 at 4188.668 kb on - strand, within Pf1N1B4_3779 at 4188.948 kb on + strand at 4188.973 kb on + strand at 4188.974 kb on - strand at 4188.974 kb on - strand at 4189.061 kb on + strand at 4189.061 kb on + strand at 4189.061 kb on + strand at 4189.079 kb on + strand at 4189.097 kb on - strand at 4189.097 kb on - strand at 4189.097 kb on - strand at 4189.098 kb on + strand at 4189.120 kb on + strand at 4189.120 kb on + strand at 4190.257 kb on + strand at 4190.257 kb on + strand at 4190.356 kb on + strand at 4190.356 kb on + strand at 4190.357 kb on - strand at 4190.357 kb on - strand at 4190.365 kb on + strand at 4190.365 kb on + strand at 4190.365 kb on + strand at 4190.408 kb on - strand at 4190.408 kb on - strand at 4190.408 kb on - strand at 4190.512 kb on + strand, within Pf1N1B4_3781 at 4190.512 kb on + strand, within Pf1N1B4_3781 at 4190.556 kb on + strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.781 kb on + strand, within Pf1N1B4_3781 at 4190.782 kb on - strand, within Pf1N1B4_3781 at 4190.794 kb on + strand, within Pf1N1B4_3781 at 4190.794 kb on + strand, within Pf1N1B4_3781 at 4190.795 kb on - strand, within Pf1N1B4_3781 at 4190.935 kb on + strand, within Pf1N1B4_3781 at 4190.935 kb on + strand, within Pf1N1B4_3781 at 4190.936 kb on - strand at 4190.936 kb on - strand, within Pf1N1B4_3781 at 4190.936 kb on - strand, within Pf1N1B4_3781 at 4190.974 kb on + strand, within Pf1N1B4_3781 at 4190.974 kb on + strand, within Pf1N1B4_3781 at 4190.974 kb on + strand at 4190.974 kb on + strand at 4190.975 kb on - strand, within Pf1N1B4_3781 at 4190.975 kb on - strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.978 kb on - strand, within Pf1N1B4_3781 at 4190.978 kb on - strand, within Pf1N1B4_3781 at 4191.033 kb on + strand at 4191.065 kb on + strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.068 kb on + strand, within Pf1N1B4_3781
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,188,476 + Pf1N1B4_3779 0.45 +0.5 4,188,648 + Pf1N1B4_3779 0.63 -0.0 4,188,648 + Pf1N1B4_3779 0.63 +0.9 4,188,667 + Pf1N1B4_3779 0.65 +1.3 4,188,668 - Pf1N1B4_3779 0.65 +1.1 4,188,948 + +1.1 4,188,973 + +0.5 4,188,974 - -0.5 4,188,974 - +1.2 4,189,061 + -0.5 4,189,061 + -2.2 4,189,061 + -0.4 4,189,079 + +2.6 4,189,097 - -1.1 4,189,097 - +1.1 4,189,097 - +0.9 4,189,098 + +0.2 4,189,120 + +0.1 4,189,120 + +1.1 4,190,257 + +0.6 4,190,257 + -0.9 4,190,356 + -0.3 4,190,356 + -0.7 4,190,357 - -0.0 4,190,357 - +0.2 4,190,365 + -0.5 4,190,365 + -0.2 4,190,365 + -1.7 4,190,408 - +1.9 4,190,408 - +0.5 4,190,408 - -0.8 4,190,512 + Pf1N1B4_3781 0.17 -1.8 4,190,512 + Pf1N1B4_3781 0.17 +0.4 4,190,556 + Pf1N1B4_3781 0.21 -0.2 4,190,557 - Pf1N1B4_3781 0.21 -0.7 4,190,557 - Pf1N1B4_3781 0.21 -0.4 4,190,557 - Pf1N1B4_3781 0.21 -0.7 4,190,781 + Pf1N1B4_3781 0.40 -0.9 4,190,782 - Pf1N1B4_3781 0.40 +0.9 4,190,794 + Pf1N1B4_3781 0.41 +0.3 4,190,794 + Pf1N1B4_3781 0.41 +0.9 4,190,795 - Pf1N1B4_3781 0.41 +1.3 4,190,935 + Pf1N1B4_3781 0.54 +3.0 4,190,935 + Pf1N1B4_3781 0.54 -0.5 4,190,936 - -0.1 4,190,936 - Pf1N1B4_3781 0.54 -2.2 4,190,936 - Pf1N1B4_3781 0.54 -0.6 4,190,974 + Pf1N1B4_3781 0.57 -1.8 4,190,974 + Pf1N1B4_3781 0.57 -0.4 4,190,974 + +2.0 4,190,974 + +1.6 4,190,975 - Pf1N1B4_3781 0.57 +0.6 4,190,975 - Pf1N1B4_3781 0.57 -0.2 4,190,977 + Pf1N1B4_3781 0.57 -0.7 4,190,977 + Pf1N1B4_3781 0.57 +0.1 4,190,977 + Pf1N1B4_3781 0.57 +2.1 4,190,977 + -1.5 4,190,977 + Pf1N1B4_3781 0.57 +0.6 4,190,978 - Pf1N1B4_3781 0.57 +0.5 4,190,978 - Pf1N1B4_3781 0.57 -0.9 4,191,033 + +0.3 4,191,065 + Pf1N1B4_3781 0.65 +0.9 4,191,066 - Pf1N1B4_3781 0.65 +0.6 4,191,066 - Pf1N1B4_3781 0.65 -1.4 4,191,066 - Pf1N1B4_3781 0.65 -0.0 4,191,066 - Pf1N1B4_3781 0.65 +1.3 4,191,066 - Pf1N1B4_3781 0.65 +0.8 4,191,068 + Pf1N1B4_3781 0.65 +1.2
Or see this region's nucleotide sequence