Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3696 and Pf1N1B4_3697 are separated by 8 nucleotides Pf1N1B4_3697 and Pf1N1B4_3698 overlap by 1 nucleotides Pf1N1B4_3698 and Pf1N1B4_3699 are separated by 389 nucleotides
Pf1N1B4_3696 - FIG00955360: hypothetical protein, at 4,104,939 to 4,105,652
_3696
Pf1N1B4_3697 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 4,105,661 to 4,106,311
_3697
Pf1N1B4_3698 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 4,106,311 to 4,107,369
_3698
Pf1N1B4_3699 - FIG00954480: hypothetical protein, at 4,107,759 to 4,108,817
_3699
Position (kb)
4106
4107
4108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4105.399 kb on + strand, within Pf1N1B4_3696 at 4105.399 kb on + strand, within Pf1N1B4_3696 at 4105.399 kb on + strand, within Pf1N1B4_3696 at 4105.567 kb on + strand, within Pf1N1B4_3696 at 4105.567 kb on + strand, within Pf1N1B4_3696 at 4105.567 kb on + strand, within Pf1N1B4_3696 at 4105.567 kb on + strand, within Pf1N1B4_3696 at 4105.568 kb on - strand, within Pf1N1B4_3696 at 4105.568 kb on - strand, within Pf1N1B4_3696 at 4105.568 kb on - strand, within Pf1N1B4_3696 at 4105.568 kb on - strand, within Pf1N1B4_3696 at 4105.652 kb on + strand at 4105.652 kb on + strand at 4105.653 kb on - strand at 4105.653 kb on - strand at 4105.653 kb on - strand at 4105.662 kb on + strand at 4105.662 kb on + strand at 4105.663 kb on - strand at 4105.663 kb on - strand at 4105.663 kb on - strand at 4105.694 kb on + strand at 4105.694 kb on + strand at 4105.694 kb on + strand at 4105.695 kb on - strand at 4105.709 kb on + strand at 4105.709 kb on + strand at 4105.709 kb on + strand at 4105.839 kb on - strand, within Pf1N1B4_3697 at 4105.839 kb on - strand, within Pf1N1B4_3697 at 4105.839 kb on - strand, within Pf1N1B4_3697 at 4105.841 kb on + strand, within Pf1N1B4_3697 at 4105.841 kb on + strand, within Pf1N1B4_3697 at 4105.841 kb on + strand, within Pf1N1B4_3697 at 4105.841 kb on + strand, within Pf1N1B4_3697 at 4105.842 kb on - strand, within Pf1N1B4_3697 at 4105.842 kb on - strand, within Pf1N1B4_3697 at 4105.850 kb on + strand, within Pf1N1B4_3697 at 4105.851 kb on - strand, within Pf1N1B4_3697 at 4105.947 kb on - strand, within Pf1N1B4_3697 at 4105.956 kb on + strand, within Pf1N1B4_3697 at 4105.956 kb on + strand, within Pf1N1B4_3697 at 4106.049 kb on - strand, within Pf1N1B4_3697 at 4106.049 kb on - strand, within Pf1N1B4_3697 at 4106.264 kb on - strand at 4106.925 kb on - strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.606 kb on + strand at 4107.606 kb on + strand at 4107.607 kb on - strand at 4107.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,105,399 + Pf1N1B4_3696 0.64 +0.2 4,105,399 + Pf1N1B4_3696 0.64 -0.2 4,105,399 + Pf1N1B4_3696 0.64 +0.2 4,105,567 + Pf1N1B4_3696 0.88 -2.0 4,105,567 + Pf1N1B4_3696 0.88 +0.3 4,105,567 + Pf1N1B4_3696 0.88 -0.7 4,105,567 + Pf1N1B4_3696 0.88 +0.2 4,105,568 - Pf1N1B4_3696 0.88 +0.8 4,105,568 - Pf1N1B4_3696 0.88 +0.6 4,105,568 - Pf1N1B4_3696 0.88 -0.0 4,105,568 - Pf1N1B4_3696 0.88 +0.4 4,105,652 + -0.5 4,105,652 + -0.5 4,105,653 - +0.0 4,105,653 - -0.6 4,105,653 - -1.7 4,105,662 + -0.8 4,105,662 + +0.8 4,105,663 - +0.0 4,105,663 - -1.6 4,105,663 - -1.0 4,105,694 + -1.2 4,105,694 + -2.9 4,105,694 + -3.3 4,105,695 - -0.1 4,105,709 + -2.4 4,105,709 + -1.7 4,105,709 + -1.4 4,105,839 - Pf1N1B4_3697 0.27 -1.2 4,105,839 - Pf1N1B4_3697 0.27 -2.1 4,105,839 - Pf1N1B4_3697 0.27 -0.7 4,105,841 + Pf1N1B4_3697 0.28 -1.7 4,105,841 + Pf1N1B4_3697 0.28 -1.7 4,105,841 + Pf1N1B4_3697 0.28 -2.2 4,105,841 + Pf1N1B4_3697 0.28 -2.3 4,105,842 - Pf1N1B4_3697 0.28 -1.2 4,105,842 - Pf1N1B4_3697 0.28 -2.8 4,105,850 + Pf1N1B4_3697 0.29 -1.6 4,105,851 - Pf1N1B4_3697 0.29 -1.2 4,105,947 - Pf1N1B4_3697 0.44 -0.8 4,105,956 + Pf1N1B4_3697 0.45 -2.3 4,105,956 + Pf1N1B4_3697 0.45 -2.4 4,106,049 - Pf1N1B4_3697 0.60 -2.7 4,106,049 - Pf1N1B4_3697 0.60 -1.6 4,106,264 - -2.4 4,106,925 - -1.4 4,107,604 + +2.0 4,107,604 + +1.1 4,107,604 + -3.5 4,107,604 + -0.4 4,107,604 + -0.7 4,107,605 - +1.6 4,107,605 - +2.3 4,107,605 - +1.6 4,107,606 + +0.9 4,107,606 + -0.5 4,107,607 - -1.2 4,107,634 + -2.1
Or see this region's nucleotide sequence