Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3567 and Pf1N1B4_3568 are separated by 313 nucleotides Pf1N1B4_3568 and Pf1N1B4_3569 are separated by 45 nucleotides Pf1N1B4_3569 and Pf1N1B4_3570 are separated by 52 nucleotides
Pf1N1B4_3567 - Phenylalanine hydroxylase transcriptional activator PhhR, at 3,969,919 to 3,971,478
_3567
Pf1N1B4_3568 - Flagellar basal-body rod protein FlgF, at 3,971,792 to 3,972,532
_3568
Pf1N1B4_3569 - Flagellar basal-body rod protein FlgG, at 3,972,578 to 3,973,363
_3569
Pf1N1B4_3570 - Flagellar L-ring protein FlgH, at 3,973,416 to 3,974,111
_3570
Position (kb)
3971
3972
3973 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3970.825 kb on - strand, within Pf1N1B4_3567 at 3970.933 kb on + strand, within Pf1N1B4_3567 at 3970.933 kb on + strand, within Pf1N1B4_3567 at 3970.933 kb on + strand, within Pf1N1B4_3567 at 3970.933 kb on + strand, within Pf1N1B4_3567 at 3970.934 kb on - strand, within Pf1N1B4_3567 at 3970.934 kb on - strand, within Pf1N1B4_3567 at 3970.934 kb on - strand, within Pf1N1B4_3567 at 3970.934 kb on - strand, within Pf1N1B4_3567 at 3970.934 kb on - strand at 3971.014 kb on + strand, within Pf1N1B4_3567 at 3971.014 kb on + strand, within Pf1N1B4_3567 at 3971.014 kb on + strand, within Pf1N1B4_3567 at 3971.014 kb on + strand, within Pf1N1B4_3567 at 3971.015 kb on - strand, within Pf1N1B4_3567 at 3971.015 kb on - strand, within Pf1N1B4_3567 at 3971.173 kb on + strand, within Pf1N1B4_3567 at 3971.210 kb on + strand, within Pf1N1B4_3567 at 3971.210 kb on + strand, within Pf1N1B4_3567 at 3971.210 kb on + strand, within Pf1N1B4_3567 at 3971.210 kb on + strand, within Pf1N1B4_3567 at 3971.210 kb on + strand, within Pf1N1B4_3567 at 3971.211 kb on - strand, within Pf1N1B4_3567 at 3971.211 kb on - strand, within Pf1N1B4_3567 at 3971.371 kb on + strand at 3971.371 kb on + strand at 3971.371 kb on + strand at 3971.372 kb on - strand at 3971.372 kb on - strand at 3971.372 kb on - strand at 3971.424 kb on + strand at 3971.452 kb on + strand at 3971.452 kb on + strand at 3971.452 kb on + strand at 3971.452 kb on + strand at 3971.452 kb on + strand at 3971.452 kb on + strand at 3971.453 kb on - strand at 3971.453 kb on - strand at 3971.453 kb on - strand at 3971.453 kb on - strand at 3971.464 kb on - strand at 3971.750 kb on - strand at 3971.750 kb on - strand at 3971.801 kb on + strand at 3971.801 kb on + strand at 3971.801 kb on + strand at 3971.801 kb on + strand at 3971.801 kb on + strand at 3971.802 kb on - strand at 3971.802 kb on - strand at 3971.808 kb on - strand at 3971.808 kb on - strand at 3971.808 kb on - strand at 3971.808 kb on - strand at 3971.808 kb on - strand at 3971.882 kb on - strand, within Pf1N1B4_3568 at 3972.086 kb on + strand, within Pf1N1B4_3568 at 3972.086 kb on + strand, within Pf1N1B4_3568 at 3972.530 kb on + strand at 3972.531 kb on - strand at 3972.531 kb on - strand at 3972.534 kb on + strand at 3972.534 kb on + strand at 3972.534 kb on + strand at 3972.535 kb on - strand at 3972.714 kb on - strand, within Pf1N1B4_3569 at 3972.714 kb on - strand, within Pf1N1B4_3569 at 3972.917 kb on + strand, within Pf1N1B4_3569 at 3972.917 kb on + strand, within Pf1N1B4_3569 at 3973.295 kb on + strand at 3973.296 kb on - strand at 3973.371 kb on + strand at 3973.371 kb on + strand at 3973.429 kb on + strand at 3973.429 kb on + strand at 3973.429 kb on + strand at 3973.429 kb on + strand at 3973.430 kb on - strand at 3973.430 kb on - strand at 3973.503 kb on + strand at 3973.503 kb on + strand, within Pf1N1B4_3570 at 3973.504 kb on - strand, within Pf1N1B4_3570 at 3973.506 kb on + strand, within Pf1N1B4_3570 at 3973.506 kb on + strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570 at 3973.507 kb on - strand, within Pf1N1B4_3570
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,970,825 - Pf1N1B4_3567 0.58 -1.8 3,970,933 + Pf1N1B4_3567 0.65 -1.4 3,970,933 + Pf1N1B4_3567 0.65 -1.5 3,970,933 + Pf1N1B4_3567 0.65 +0.1 3,970,933 + Pf1N1B4_3567 0.65 +0.5 3,970,934 - Pf1N1B4_3567 0.65 +1.3 3,970,934 - Pf1N1B4_3567 0.65 +0.9 3,970,934 - Pf1N1B4_3567 0.65 +0.5 3,970,934 - Pf1N1B4_3567 0.65 -1.3 3,970,934 - -0.4 3,971,014 + Pf1N1B4_3567 0.70 -0.1 3,971,014 + Pf1N1B4_3567 0.70 +0.3 3,971,014 + Pf1N1B4_3567 0.70 +0.7 3,971,014 + Pf1N1B4_3567 0.70 +0.6 3,971,015 - Pf1N1B4_3567 0.70 -0.2 3,971,015 - Pf1N1B4_3567 0.70 -0.0 3,971,173 + Pf1N1B4_3567 0.80 -0.5 3,971,210 + Pf1N1B4_3567 0.83 +0.6 3,971,210 + Pf1N1B4_3567 0.83 -0.1 3,971,210 + Pf1N1B4_3567 0.83 +0.1 3,971,210 + Pf1N1B4_3567 0.83 -0.0 3,971,210 + Pf1N1B4_3567 0.83 +0.2 3,971,211 - Pf1N1B4_3567 0.83 +0.2 3,971,211 - Pf1N1B4_3567 0.83 +0.7 3,971,371 + -0.0 3,971,371 + +0.7 3,971,371 + -0.3 3,971,372 - +0.6 3,971,372 - -0.2 3,971,372 - +0.6 3,971,424 + -1.4 3,971,452 + -0.0 3,971,452 + +0.6 3,971,452 + +0.1 3,971,452 + +0.5 3,971,452 + -0.1 3,971,452 + +0.5 3,971,453 - +1.2 3,971,453 - +2.9 3,971,453 - -0.8 3,971,453 - -0.0 3,971,464 - +2.1 3,971,750 - -1.6 3,971,750 - +0.6 3,971,801 + +0.7 3,971,801 + +0.8 3,971,801 + +3.1 3,971,801 + +0.8 3,971,801 + -0.2 3,971,802 - +1.1 3,971,802 - +1.0 3,971,808 - -0.0 3,971,808 - -0.8 3,971,808 - -2.3 3,971,808 - -0.6 3,971,808 - -0.4 3,971,882 - Pf1N1B4_3568 0.12 +1.0 3,972,086 + Pf1N1B4_3568 0.40 -1.6 3,972,086 + Pf1N1B4_3568 0.40 -0.0 3,972,530 + -0.8 3,972,531 - +1.0 3,972,531 - +0.4 3,972,534 + -1.8 3,972,534 + -0.3 3,972,534 + +0.2 3,972,535 - -0.2 3,972,714 - Pf1N1B4_3569 0.17 -2.3 3,972,714 - Pf1N1B4_3569 0.17 -0.8 3,972,917 + Pf1N1B4_3569 0.43 -0.3 3,972,917 + Pf1N1B4_3569 0.43 +1.0 3,973,295 + -0.8 3,973,296 - +0.8 3,973,371 + +0.3 3,973,371 + -0.6 3,973,429 + -0.0 3,973,429 + +1.3 3,973,429 + -1.8 3,973,429 + -0.4 3,973,430 - +1.6 3,973,430 - +0.2 3,973,503 + +0.6 3,973,503 + Pf1N1B4_3570 0.12 -0.2 3,973,504 - Pf1N1B4_3570 0.13 +0.6 3,973,506 + Pf1N1B4_3570 0.13 +0.8 3,973,506 + Pf1N1B4_3570 0.13 -0.9 3,973,507 - Pf1N1B4_3570 0.13 +1.9 3,973,507 - Pf1N1B4_3570 0.13 -0.4 3,973,507 - Pf1N1B4_3570 0.13 -0.1 3,973,507 - Pf1N1B4_3570 0.13 -1.8 3,973,507 - Pf1N1B4_3570 0.13 +1.9 3,973,507 - Pf1N1B4_3570 0.13 -0.8 3,973,507 - Pf1N1B4_3570 0.13 -2.2
Or see this region's nucleotide sequence