Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3516 and Pf1N1B4_3517 are separated by 61 nucleotides Pf1N1B4_3517 and Pf1N1B4_3518 are separated by 15 nucleotides Pf1N1B4_3518 and Pf1N1B4_3519 are separated by 31 nucleotides Pf1N1B4_3519 and Pf1N1B4_3520 overlap by 4 nucleotides
Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547
_3516
Pf1N1B4_3517 - hypothetical protein, at 3,925,609 to 3,925,827
_3517
Pf1N1B4_3518 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,925,843 to 3,926,769
_3518
Pf1N1B4_3519 - Lipase (EC 3.1.1.3), at 3,926,801 to 3,927,148
_3519
Pf1N1B4_3520 - Phosphohistidine phosphatase SixA, at 3,927,145 to 3,927,597
_3520
Position (kb)
3925
3926
3927 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3924.995 kb on - strand, within Pf1N1B4_3516 at 3925.007 kb on + strand, within Pf1N1B4_3516 at 3925.169 kb on + strand, within Pf1N1B4_3516 at 3925.205 kb on - strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.268 kb on - strand at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.557 kb on + strand at 3925.557 kb on + strand at 3925.558 kb on - strand at 3925.558 kb on - strand at 3925.651 kb on + strand at 3925.663 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.743 kb on + strand, within Pf1N1B4_3517 at 3926.606 kb on + strand, within Pf1N1B4_3518 at 3926.607 kb on - strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.708 kb on + strand at 3926.745 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.768 kb on - strand at 3926.768 kb on - strand at 3926.771 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.920 kb on - strand, within Pf1N1B4_3519 at 3926.931 kb on - strand, within Pf1N1B4_3519 at 3926.942 kb on + strand, within Pf1N1B4_3519 at 3927.144 kb on + strand at 3927.159 kb on - strand at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.287 kb on - strand, within Pf1N1B4_3520 at 3927.419 kb on + strand, within Pf1N1B4_3520 at 3927.478 kb on + strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.639 kb on + strand at 3927.640 kb on - strand at 3927.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,924,995 - Pf1N1B4_3516 0.74 -0.1 3,925,007 + Pf1N1B4_3516 0.75 +1.7 3,925,169 + Pf1N1B4_3516 0.82 -0.0 3,925,205 - Pf1N1B4_3516 0.84 -0.2 3,925,267 + Pf1N1B4_3516 0.87 +0.3 3,925,267 + Pf1N1B4_3516 0.87 +0.7 3,925,267 + Pf1N1B4_3516 0.87 +0.2 3,925,267 + Pf1N1B4_3516 0.87 -0.5 3,925,267 + Pf1N1B4_3516 0.87 -0.2 3,925,267 + Pf1N1B4_3516 0.87 +0.2 3,925,268 - +0.2 3,925,268 - Pf1N1B4_3516 0.87 +0.6 3,925,268 - Pf1N1B4_3516 0.87 +1.0 3,925,557 + +0.9 3,925,557 + +1.0 3,925,558 - -0.4 3,925,558 - -1.2 3,925,651 + -0.0 3,925,663 + Pf1N1B4_3517 0.25 +0.4 3,925,701 + Pf1N1B4_3517 0.42 +0.3 3,925,701 + Pf1N1B4_3517 0.42 -0.2 3,925,743 + Pf1N1B4_3517 0.61 -0.0 3,926,606 + Pf1N1B4_3518 0.82 -1.2 3,926,607 - Pf1N1B4_3518 0.82 -1.8 3,926,638 + Pf1N1B4_3518 0.86 -0.4 3,926,638 + Pf1N1B4_3518 0.86 -1.9 3,926,639 - Pf1N1B4_3518 0.86 -0.8 3,926,639 - Pf1N1B4_3518 0.86 -1.4 3,926,639 - Pf1N1B4_3518 0.86 -0.2 3,926,639 - Pf1N1B4_3518 0.86 -1.8 3,926,639 - Pf1N1B4_3518 0.86 -1.0 3,926,708 + -0.0 3,926,745 + +0.3 3,926,767 + -1.9 3,926,767 + +0.8 3,926,767 + -0.3 3,926,767 + -0.2 3,926,767 + -0.0 3,926,767 + +0.2 3,926,767 + +2.0 3,926,767 + -0.2 3,926,767 + -2.4 3,926,768 - +0.2 3,926,768 - -0.2 3,926,771 + -0.4 3,926,781 + -1.1 3,926,781 + +0.8 3,926,781 + +0.8 3,926,781 + +3.4 3,926,781 + -0.8 3,926,782 - -0.4 3,926,782 - -0.6 3,926,782 - +0.6 3,926,782 - +0.6 3,926,828 + +0.4 3,926,828 + +0.2 3,926,828 + +0.1 3,926,828 + -1.9 3,926,867 + Pf1N1B4_3519 0.19 -1.8 3,926,867 + Pf1N1B4_3519 0.19 +0.1 3,926,867 + -0.8 3,926,919 + Pf1N1B4_3519 0.34 +1.1 3,926,919 + Pf1N1B4_3519 0.34 +0.6 3,926,919 + Pf1N1B4_3519 0.34 +1.6 3,926,919 + Pf1N1B4_3519 0.34 -0.3 3,926,920 - Pf1N1B4_3519 0.34 -0.4 3,926,931 - Pf1N1B4_3519 0.37 +0.1 3,926,942 + Pf1N1B4_3519 0.41 -0.9 3,927,144 + -2.1 3,927,159 - -1.0 3,927,286 + Pf1N1B4_3520 0.31 +1.1 3,927,286 + Pf1N1B4_3520 0.31 -0.4 3,927,286 + Pf1N1B4_3520 0.31 -0.6 3,927,286 + Pf1N1B4_3520 0.31 +0.6 3,927,286 + Pf1N1B4_3520 0.31 -0.9 3,927,286 + Pf1N1B4_3520 0.31 -0.8 3,927,287 - Pf1N1B4_3520 0.31 +0.8 3,927,419 + Pf1N1B4_3520 0.60 -1.6 3,927,478 + Pf1N1B4_3520 0.74 -0.1 3,927,479 - Pf1N1B4_3520 0.74 +0.4 3,927,479 - Pf1N1B4_3520 0.74 +0.6 3,927,479 - Pf1N1B4_3520 0.74 -0.3 3,927,639 + -1.4 3,927,640 - -0.1 3,927,727 - -1.5
Or see this region's nucleotide sequence