Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3514 and Pf1N1B4_3515 overlap by 4 nucleotides Pf1N1B4_3515 and Pf1N1B4_3516 are separated by 1 nucleotides Pf1N1B4_3516 and Pf1N1B4_3517 are separated by 61 nucleotides Pf1N1B4_3517 and Pf1N1B4_3518 are separated by 15 nucleotides
Pf1N1B4_3514 - Sensor histidine kinase/response regulator, at 3,920,592 to 3,922,493
_3514
Pf1N1B4_3515 - FIG00953467: hypothetical protein, at 3,922,490 to 3,923,422
_3515
Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547
_3516
Pf1N1B4_3517 - hypothetical protein, at 3,925,609 to 3,925,827
_3517
Pf1N1B4_3518 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,925,843 to 3,926,769
_3518
Position (kb)
3923
3924
3925
3926 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3922.463 kb on + strand at 3922.552 kb on + strand at 3922.583 kb on + strand at 3922.583 kb on + strand at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.687 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.791 kb on + strand, within Pf1N1B4_3515 at 3922.792 kb on - strand, within Pf1N1B4_3515 at 3923.165 kb on + strand, within Pf1N1B4_3515 at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.450 kb on - strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.471 kb on - strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.620 kb on + strand at 3923.621 kb on - strand at 3923.621 kb on - strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.623 kb on + strand at 3923.624 kb on - strand at 3923.661 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.734 kb on + strand, within Pf1N1B4_3516 at 3923.735 kb on - strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.740 kb on + strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.741 kb on - strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.833 kb on + strand, within Pf1N1B4_3516 at 3923.834 kb on - strand, within Pf1N1B4_3516 at 3923.834 kb on - strand, within Pf1N1B4_3516 at 3923.881 kb on + strand, within Pf1N1B4_3516 at 3923.932 kb on + strand, within Pf1N1B4_3516 at 3923.932 kb on + strand, within Pf1N1B4_3516 at 3923.998 kb on + strand, within Pf1N1B4_3516 at 3923.998 kb on + strand, within Pf1N1B4_3516 at 3924.049 kb on + strand, within Pf1N1B4_3516 at 3924.103 kb on + strand, within Pf1N1B4_3516 at 3924.104 kb on - strand, within Pf1N1B4_3516 at 3924.139 kb on + strand, within Pf1N1B4_3516 at 3924.139 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.193 kb on + strand, within Pf1N1B4_3516 at 3924.194 kb on - strand, within Pf1N1B4_3516 at 3924.194 kb on - strand at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.289 kb on + strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.290 kb on - strand, within Pf1N1B4_3516 at 3924.319 kb on + strand, within Pf1N1B4_3516 at 3924.319 kb on + strand, within Pf1N1B4_3516 at 3924.385 kb on + strand, within Pf1N1B4_3516 at 3924.550 kb on + strand, within Pf1N1B4_3516 at 3924.550 kb on + strand, within Pf1N1B4_3516 at 3924.551 kb on - strand, within Pf1N1B4_3516 at 3924.659 kb on + strand, within Pf1N1B4_3516 at 3924.659 kb on + strand, within Pf1N1B4_3516 at 3924.659 kb on + strand, within Pf1N1B4_3516 at 3924.660 kb on - strand, within Pf1N1B4_3516 at 3924.661 kb on + strand, within Pf1N1B4_3516 at 3924.662 kb on - strand, within Pf1N1B4_3516 at 3924.662 kb on - strand, within Pf1N1B4_3516 at 3924.712 kb on + strand, within Pf1N1B4_3516 at 3924.712 kb on + strand, within Pf1N1B4_3516 at 3924.713 kb on - strand at 3924.775 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.779 kb on + strand, within Pf1N1B4_3516 at 3924.781 kb on + strand, within Pf1N1B4_3516 at 3924.781 kb on + strand, within Pf1N1B4_3516 at 3924.781 kb on + strand, within Pf1N1B4_3516 at 3924.995 kb on - strand, within Pf1N1B4_3516 at 3925.007 kb on + strand, within Pf1N1B4_3516 at 3925.169 kb on + strand, within Pf1N1B4_3516 at 3925.205 kb on - strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.268 kb on - strand at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.557 kb on + strand at 3925.557 kb on + strand at 3925.558 kb on - strand at 3925.558 kb on - strand at 3925.651 kb on + strand at 3925.663 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.743 kb on + strand, within Pf1N1B4_3517
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,922,463 + +0.8 3,922,552 + +1.0 3,922,583 + -0.2 3,922,583 + -1.2 3,922,584 - Pf1N1B4_3515 0.10 -2.0 3,922,584 - Pf1N1B4_3515 0.10 -1.8 3,922,683 + Pf1N1B4_3515 0.21 +0.5 3,922,683 + Pf1N1B4_3515 0.21 +0.4 3,922,683 + Pf1N1B4_3515 0.21 -0.8 3,922,683 + Pf1N1B4_3515 0.21 +1.1 3,922,683 + Pf1N1B4_3515 0.21 +0.4 3,922,684 - Pf1N1B4_3515 0.21 +0.2 3,922,684 - Pf1N1B4_3515 0.21 -1.8 3,922,684 - Pf1N1B4_3515 0.21 -1.0 3,922,687 + Pf1N1B4_3515 0.21 +0.8 3,922,752 + Pf1N1B4_3515 0.28 +1.1 3,922,752 + Pf1N1B4_3515 0.28 +0.7 3,922,752 + Pf1N1B4_3515 0.28 +0.4 3,922,752 + Pf1N1B4_3515 0.28 -0.1 3,922,752 + Pf1N1B4_3515 0.28 -0.0 3,922,791 + Pf1N1B4_3515 0.32 +0.7 3,922,792 - Pf1N1B4_3515 0.32 -0.8 3,923,165 + Pf1N1B4_3515 0.72 +1.3 3,923,425 + -1.2 3,923,425 + +0.6 3,923,425 + +0.3 3,923,425 + +0.8 3,923,425 + +0.1 3,923,425 + +0.1 3,923,425 + +2.1 3,923,425 + +0.8 3,923,426 - -1.8 3,923,426 - +0.9 3,923,426 - -0.1 3,923,426 - -1.1 3,923,449 + +0.1 3,923,449 + +0.9 3,923,449 + +0.5 3,923,449 + +3.1 3,923,450 - +0.3 3,923,470 + -2.6 3,923,470 + +2.8 3,923,470 + +0.7 3,923,470 + -1.4 3,923,470 + +0.9 3,923,471 - +0.7 3,923,620 + +0.4 3,923,620 + +2.5 3,923,620 + +2.1 3,923,620 + +0.8 3,923,620 + -0.0 3,923,621 - +0.8 3,923,621 - -0.0 3,923,623 + +1.0 3,923,623 + +0.8 3,923,623 + +0.1 3,923,623 + +0.9 3,923,624 - +1.1 3,923,661 + Pf1N1B4_3516 0.11 +0.2 3,923,734 + Pf1N1B4_3516 0.15 +0.6 3,923,734 + Pf1N1B4_3516 0.15 +1.4 3,923,734 + Pf1N1B4_3516 0.15 +0.6 3,923,734 + Pf1N1B4_3516 0.15 +1.3 3,923,734 + Pf1N1B4_3516 0.15 +0.9 3,923,734 + Pf1N1B4_3516 0.15 +2.7 3,923,734 + Pf1N1B4_3516 0.15 +0.7 3,923,734 + Pf1N1B4_3516 0.15 -0.1 3,923,735 - Pf1N1B4_3516 0.15 -0.0 3,923,740 + Pf1N1B4_3516 0.15 +0.6 3,923,740 + Pf1N1B4_3516 0.15 +0.1 3,923,740 + Pf1N1B4_3516 0.15 +0.9 3,923,740 + Pf1N1B4_3516 0.15 +0.8 3,923,740 + Pf1N1B4_3516 0.15 +1.6 3,923,740 + Pf1N1B4_3516 0.15 -1.1 3,923,740 + Pf1N1B4_3516 0.15 -0.8 3,923,740 + +2.0 3,923,740 + Pf1N1B4_3516 0.15 -0.2 3,923,740 + Pf1N1B4_3516 0.15 +0.3 3,923,740 + Pf1N1B4_3516 0.15 +0.4 3,923,740 + Pf1N1B4_3516 0.15 +1.0 3,923,740 + Pf1N1B4_3516 0.15 +0.2 3,923,740 + Pf1N1B4_3516 0.15 +0.6 3,923,741 - Pf1N1B4_3516 0.15 +0.2 3,923,741 - Pf1N1B4_3516 0.15 +0.6 3,923,741 - Pf1N1B4_3516 0.15 +0.4 3,923,833 + Pf1N1B4_3516 0.19 +0.1 3,923,833 + Pf1N1B4_3516 0.19 +0.3 3,923,833 + Pf1N1B4_3516 0.19 +1.1 3,923,833 + Pf1N1B4_3516 0.19 +1.7 3,923,834 - Pf1N1B4_3516 0.19 +0.6 3,923,834 - Pf1N1B4_3516 0.19 +0.6 3,923,881 + Pf1N1B4_3516 0.22 +0.8 3,923,932 + Pf1N1B4_3516 0.24 +2.7 3,923,932 + Pf1N1B4_3516 0.24 +0.5 3,923,998 + Pf1N1B4_3516 0.27 +1.8 3,923,998 + Pf1N1B4_3516 0.27 -0.0 3,924,049 + Pf1N1B4_3516 0.29 +1.0 3,924,103 + Pf1N1B4_3516 0.32 +1.2 3,924,104 - Pf1N1B4_3516 0.32 +0.4 3,924,139 + Pf1N1B4_3516 0.34 +0.3 3,924,139 + Pf1N1B4_3516 0.34 +1.4 3,924,193 + Pf1N1B4_3516 0.36 -0.0 3,924,193 + Pf1N1B4_3516 0.36 +0.5 3,924,193 + Pf1N1B4_3516 0.36 +1.6 3,924,193 + Pf1N1B4_3516 0.36 +1.0 3,924,194 - Pf1N1B4_3516 0.36 +1.1 3,924,194 - +0.6 3,924,289 + Pf1N1B4_3516 0.41 +0.8 3,924,289 + Pf1N1B4_3516 0.41 +1.2 3,924,289 + Pf1N1B4_3516 0.41 +0.8 3,924,289 + Pf1N1B4_3516 0.41 +0.9 3,924,289 + Pf1N1B4_3516 0.41 -0.0 3,924,290 - Pf1N1B4_3516 0.41 +1.2 3,924,290 - Pf1N1B4_3516 0.41 -1.0 3,924,290 - Pf1N1B4_3516 0.41 -0.5 3,924,290 - Pf1N1B4_3516 0.41 -0.1 3,924,319 + Pf1N1B4_3516 0.42 -0.8 3,924,319 + Pf1N1B4_3516 0.42 +0.8 3,924,385 + Pf1N1B4_3516 0.45 -0.2 3,924,550 + Pf1N1B4_3516 0.53 +0.3 3,924,550 + Pf1N1B4_3516 0.53 -0.2 3,924,551 - Pf1N1B4_3516 0.53 +0.6 3,924,659 + Pf1N1B4_3516 0.58 -0.4 3,924,659 + Pf1N1B4_3516 0.58 +1.3 3,924,659 + Pf1N1B4_3516 0.58 +1.6 3,924,660 - Pf1N1B4_3516 0.58 +1.3 3,924,661 + Pf1N1B4_3516 0.58 -0.0 3,924,662 - Pf1N1B4_3516 0.58 -2.5 3,924,662 - Pf1N1B4_3516 0.58 +0.2 3,924,712 + Pf1N1B4_3516 0.61 +2.7 3,924,712 + Pf1N1B4_3516 0.61 -0.3 3,924,713 - +1.1 3,924,775 + Pf1N1B4_3516 0.64 +1.1 3,924,779 + Pf1N1B4_3516 0.64 +0.0 3,924,779 + Pf1N1B4_3516 0.64 -0.2 3,924,779 + Pf1N1B4_3516 0.64 -0.0 3,924,779 + Pf1N1B4_3516 0.64 +1.0 3,924,779 + Pf1N1B4_3516 0.64 +0.1 3,924,779 + Pf1N1B4_3516 0.64 +1.4 3,924,779 + Pf1N1B4_3516 0.64 +0.8 3,924,779 + Pf1N1B4_3516 0.64 +0.0 3,924,781 + Pf1N1B4_3516 0.64 +0.4 3,924,781 + Pf1N1B4_3516 0.64 +0.1 3,924,781 + Pf1N1B4_3516 0.64 +0.6 3,924,995 - Pf1N1B4_3516 0.74 -0.1 3,925,007 + Pf1N1B4_3516 0.75 +1.7 3,925,169 + Pf1N1B4_3516 0.82 -0.0 3,925,205 - Pf1N1B4_3516 0.84 -0.2 3,925,267 + Pf1N1B4_3516 0.87 +0.3 3,925,267 + Pf1N1B4_3516 0.87 +0.7 3,925,267 + Pf1N1B4_3516 0.87 +0.2 3,925,267 + Pf1N1B4_3516 0.87 -0.5 3,925,267 + Pf1N1B4_3516 0.87 -0.2 3,925,267 + Pf1N1B4_3516 0.87 +0.2 3,925,268 - +0.2 3,925,268 - Pf1N1B4_3516 0.87 +0.6 3,925,268 - Pf1N1B4_3516 0.87 +1.0 3,925,557 + +0.9 3,925,557 + +1.0 3,925,558 - -0.4 3,925,558 - -1.2 3,925,651 + -0.0 3,925,663 + Pf1N1B4_3517 0.25 +0.4 3,925,701 + Pf1N1B4_3517 0.42 +0.3 3,925,701 + Pf1N1B4_3517 0.42 -0.2 3,925,743 + Pf1N1B4_3517 0.61 -0.0
Or see this region's nucleotide sequence