Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3434 and Pf1N1B4_3435 are separated by 27 nucleotides Pf1N1B4_3435 and Pf1N1B4_3436 are separated by 188 nucleotides Pf1N1B4_3436 and Pf1N1B4_3437 overlap by 24 nucleotides
Pf1N1B4_3434 - Succinylglutamate desuccinylase/aspartoacylase, at 3,827,089 to 3,828,201
_3434
Pf1N1B4_3435 - ABC transporter for L-Arginine and L-Citrulline, ATPase component (from data) , at 3,828,229 to 3,828,993
_3435
Pf1N1B4_3436 - hypothetical protein, at 3,829,182 to 3,829,337
_3436
Pf1N1B4_3437 - Arginine pathway regulatory protein ArgR, repressor of arg regulon, at 3,829,314 to 3,830,219
_3437
Position (kb)
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3827.620 kb on + strand, within Pf1N1B4_3434 at 3827.620 kb on + strand, within Pf1N1B4_3434 at 3827.620 kb on + strand at 3827.620 kb on + strand, within Pf1N1B4_3434 at 3827.620 kb on + strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.621 kb on - strand, within Pf1N1B4_3434 at 3827.852 kb on - strand, within Pf1N1B4_3434 at 3828.088 kb on - strand, within Pf1N1B4_3434 at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.232 kb on + strand at 3828.258 kb on + strand at 3828.258 kb on + strand at 3828.265 kb on + strand at 3828.266 kb on - strand at 3828.357 kb on + strand, within Pf1N1B4_3435 at 3828.357 kb on + strand, within Pf1N1B4_3435 at 3828.358 kb on - strand, within Pf1N1B4_3435 at 3828.415 kb on - strand, within Pf1N1B4_3435 at 3828.625 kb on + strand, within Pf1N1B4_3435 at 3828.625 kb on + strand, within Pf1N1B4_3435 at 3828.991 kb on + strand at 3828.991 kb on + strand at 3828.992 kb on - strand at 3828.992 kb on - strand at 3829.280 kb on + strand, within Pf1N1B4_3436 at 3829.281 kb on - strand, within Pf1N1B4_3436 at 3829.281 kb on - strand, within Pf1N1B4_3436 at 3829.350 kb on + strand at 3829.351 kb on - strand at 3829.532 kb on - strand, within Pf1N1B4_3437 at 3829.558 kb on - strand, within Pf1N1B4_3437 at 3829.945 kb on + strand, within Pf1N1B4_3437 at 3829.980 kb on + strand, within Pf1N1B4_3437 at 3829.980 kb on + strand, within Pf1N1B4_3437 at 3829.981 kb on - strand, within Pf1N1B4_3437 at 3829.981 kb on - strand, within Pf1N1B4_3437 at 3829.981 kb on - strand, within Pf1N1B4_3437 at 3829.981 kb on - strand, within Pf1N1B4_3437 at 3829.981 kb on - strand, within Pf1N1B4_3437
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,827,620 + Pf1N1B4_3434 0.48 -0.3 3,827,620 + Pf1N1B4_3434 0.48 -1.1 3,827,620 + +0.6 3,827,620 + Pf1N1B4_3434 0.48 -0.8 3,827,620 + Pf1N1B4_3434 0.48 -0.3 3,827,621 - Pf1N1B4_3434 0.48 +0.7 3,827,621 - Pf1N1B4_3434 0.48 +0.2 3,827,621 - Pf1N1B4_3434 0.48 +1.3 3,827,621 - Pf1N1B4_3434 0.48 +1.2 3,827,621 - Pf1N1B4_3434 0.48 -0.6 3,827,621 - Pf1N1B4_3434 0.48 +0.1 3,827,621 - Pf1N1B4_3434 0.48 +0.8 3,827,621 - Pf1N1B4_3434 0.48 -0.8 3,827,621 - Pf1N1B4_3434 0.48 -0.6 3,827,621 - Pf1N1B4_3434 0.48 +1.1 3,827,621 - Pf1N1B4_3434 0.48 -0.2 3,827,621 - Pf1N1B4_3434 0.48 -1.9 3,827,621 - Pf1N1B4_3434 0.48 +1.0 3,827,852 - Pf1N1B4_3434 0.69 +0.1 3,828,088 - Pf1N1B4_3434 0.90 -0.4 3,828,232 + -0.8 3,828,232 + +0.9 3,828,232 + +2.3 3,828,232 + +0.5 3,828,232 + -1.0 3,828,232 + +0.1 3,828,232 + +1.1 3,828,258 + +1.1 3,828,258 + -1.0 3,828,265 + +1.6 3,828,266 - -0.0 3,828,357 + Pf1N1B4_3435 0.17 -1.4 3,828,357 + Pf1N1B4_3435 0.17 +0.1 3,828,358 - Pf1N1B4_3435 0.17 -0.0 3,828,415 - Pf1N1B4_3435 0.24 -0.8 3,828,625 + Pf1N1B4_3435 0.52 -0.4 3,828,625 + Pf1N1B4_3435 0.52 -1.6 3,828,991 + +0.6 3,828,991 + +0.2 3,828,992 - -1.3 3,828,992 - +0.3 3,829,280 + Pf1N1B4_3436 0.63 +2.4 3,829,281 - Pf1N1B4_3436 0.63 -0.4 3,829,281 - Pf1N1B4_3436 0.63 +0.2 3,829,350 + -0.8 3,829,351 - -1.3 3,829,532 - Pf1N1B4_3437 0.24 -0.4 3,829,558 - Pf1N1B4_3437 0.27 -0.0 3,829,945 + Pf1N1B4_3437 0.70 +0.9 3,829,980 + Pf1N1B4_3437 0.74 +0.1 3,829,980 + Pf1N1B4_3437 0.74 +1.6 3,829,981 - Pf1N1B4_3437 0.74 +1.6 3,829,981 - Pf1N1B4_3437 0.74 +0.4 3,829,981 - Pf1N1B4_3437 0.74 +2.6 3,829,981 - Pf1N1B4_3437 0.74 -0.3 3,829,981 - Pf1N1B4_3437 0.74 -1.2
Or see this region's nucleotide sequence