Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3327

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3326 and Pf1N1B4_3327 are separated by 77 nucleotidesPf1N1B4_3327 and Pf1N1B4_3328 are separated by 97 nucleotidesPf1N1B4_3328 and Pf1N1B4_3329 are separated by 31 nucleotides Pf1N1B4_3326 - HtrA protease/chaperone protein, at 3,713,929 to 3,715,359 _3326 Pf1N1B4_3327 - Exported zinc metalloprotease YfgC precursor, at 3,715,437 to 3,716,870 _3327 Pf1N1B4_3328 - Rhodanese-like domain protein, at 3,716,968 to 3,717,207 _3328 Pf1N1B4_3329 - Putative permease PerM (= YfgO), at 3,717,239 to 3,718,309 _3329 Position (kb) 3715 3716 3717Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3716.899 kb on - strandat 3716.899 kb on - strandat 3716.899 kb on - strandat 3716.930 kb on + strandat 3716.935 kb on + strandat 3716.981 kb on + strandat 3717.077 kb on + strand, within Pf1N1B4_3328at 3717.147 kb on + strand, within Pf1N1B4_3328at 3717.147 kb on + strand, within Pf1N1B4_3328at 3717.147 kb on + strand, within Pf1N1B4_3328at 3717.227 kb on + strandat 3717.227 kb on + strandat 3717.234 kb on + strandat 3717.234 kb on + strandat 3717.272 kb on + strandat 3717.325 kb on + strandat 3717.491 kb on - strand, within Pf1N1B4_3329at 3717.731 kb on + strand, within Pf1N1B4_3329at 3717.731 kb on + strandat 3717.732 kb on - strand, within Pf1N1B4_3329at 3717.732 kb on - strand, within Pf1N1B4_3329at 3717.806 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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3,716,899 - -1.9
3,716,899 - +0.7
3,716,899 - -1.3
3,716,930 + +1.0
3,716,935 + -0.8
3,716,981 + -2.5
3,717,077 + Pf1N1B4_3328 0.45 -1.9
3,717,147 + Pf1N1B4_3328 0.75 -0.8
3,717,147 + Pf1N1B4_3328 0.75 +0.2
3,717,147 + Pf1N1B4_3328 0.75 -1.0
3,717,227 + -1.2
3,717,227 + -1.0
3,717,234 + +0.3
3,717,234 + -0.2
3,717,272 + +2.9
3,717,325 + +2.0
3,717,491 - Pf1N1B4_3329 0.24 +1.7
3,717,731 + Pf1N1B4_3329 0.46 -0.4
3,717,731 + +1.2
3,717,732 - Pf1N1B4_3329 0.46 -1.5
3,717,732 - Pf1N1B4_3329 0.46 -1.6
3,717,806 + +1.6

Or see this region's nucleotide sequence