Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3326 and Pf1N1B4_3327 are separated by 77 nucleotides Pf1N1B4_3327 and Pf1N1B4_3328 are separated by 97 nucleotides Pf1N1B4_3328 and Pf1N1B4_3329 are separated by 31 nucleotides
Pf1N1B4_3326 - HtrA protease/chaperone protein, at 3,713,929 to 3,715,359
_3326
Pf1N1B4_3327 - Exported zinc metalloprotease YfgC precursor, at 3,715,437 to 3,716,870
_3327
Pf1N1B4_3328 - Rhodanese-like domain protein, at 3,716,968 to 3,717,207
_3328
Pf1N1B4_3329 - Putative permease PerM (= YfgO), at 3,717,239 to 3,718,309
_3329
Position (kb)
3715
3716
3717 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3716.899 kb on - strand at 3716.899 kb on - strand at 3716.899 kb on - strand at 3716.930 kb on + strand at 3716.935 kb on + strand at 3716.981 kb on + strand at 3717.077 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.227 kb on + strand at 3717.227 kb on + strand at 3717.234 kb on + strand at 3717.234 kb on + strand at 3717.272 kb on + strand at 3717.325 kb on + strand at 3717.491 kb on - strand, within Pf1N1B4_3329 at 3717.731 kb on + strand, within Pf1N1B4_3329 at 3717.731 kb on + strand at 3717.732 kb on - strand, within Pf1N1B4_3329 at 3717.732 kb on - strand, within Pf1N1B4_3329 at 3717.806 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,716,899 - -1.9 3,716,899 - +0.7 3,716,899 - -1.3 3,716,930 + +1.0 3,716,935 + -0.8 3,716,981 + -2.5 3,717,077 + Pf1N1B4_3328 0.45 -1.9 3,717,147 + Pf1N1B4_3328 0.75 -0.8 3,717,147 + Pf1N1B4_3328 0.75 +0.2 3,717,147 + Pf1N1B4_3328 0.75 -1.0 3,717,227 + -1.2 3,717,227 + -1.0 3,717,234 + +0.3 3,717,234 + -0.2 3,717,272 + +2.9 3,717,325 + +2.0 3,717,491 - Pf1N1B4_3329 0.24 +1.7 3,717,731 + Pf1N1B4_3329 0.46 -0.4 3,717,731 + +1.2 3,717,732 - Pf1N1B4_3329 0.46 -1.5 3,717,732 - Pf1N1B4_3329 0.46 -1.6 3,717,806 + +1.6
Or see this region's nucleotide sequence