Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3119 and Pf1N1B4_3120 are separated by 29 nucleotides Pf1N1B4_3120 and Pf1N1B4_3121 overlap by 35 nucleotides Pf1N1B4_3121 and Pf1N1B4_3122 overlap by 4 nucleotides Pf1N1B4_3122 and Pf1N1B4_3123 overlap by 1 nucleotides
Pf1N1B4_3119 - Holin, at 3,507,348 to 3,507,695
_3119
Pf1N1B4_3120 - hypothetical protein, at 3,507,725 to 3,507,859
_3120
Pf1N1B4_3121 - FIG111678: IS, phage, Tn; Transposon-related functions, at 3,507,825 to 3,508,409
_3121
Pf1N1B4_3122 - Mu-like prophage FluMu protein GP38, at 3,508,406 to 3,508,588
_3122
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Position (kb)
3508
3509 Strain fitness (log2 ratio)
-1
0
1 at 3507.541 kb on - strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.625 kb on - strand at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.825 kb on - strand at 3507.913 kb on + strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.948 kb on + strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.137 kb on + strand, within Pf1N1B4_3121 at 3508.152 kb on + strand, within Pf1N1B4_3121 at 3508.153 kb on - strand, within Pf1N1B4_3121 at 3508.399 kb on + strand at 3508.399 kb on + strand at 3508.587 kb on - strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.640 kb on - strand at 3508.640 kb on - strand at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.813 kb on + strand, within Pf1N1B4_3123 at 3508.814 kb on - strand, within Pf1N1B4_3123 at 3508.814 kb on - strand, within Pf1N1B4_3123 at 3508.945 kb on + strand, within Pf1N1B4_3123 at 3508.945 kb on + strand at 3508.945 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123 at 3509.374 kb on + strand, within Pf1N1B4_3123
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,507,541 - Pf1N1B4_3119 0.55 -0.6 3,507,624 + Pf1N1B4_3119 0.79 -1.2 3,507,624 + Pf1N1B4_3119 0.79 +0.3 3,507,625 - +1.6 3,507,824 + Pf1N1B4_3120 0.73 +1.0 3,507,824 + Pf1N1B4_3120 0.73 -1.4 3,507,824 + Pf1N1B4_3120 0.73 -1.6 3,507,825 - +0.4 3,507,913 + Pf1N1B4_3121 0.15 +0.7 3,507,914 - Pf1N1B4_3121 0.15 +1.2 3,507,914 - Pf1N1B4_3121 0.15 -0.1 3,507,914 - Pf1N1B4_3121 0.15 -0.4 3,507,948 + Pf1N1B4_3121 0.21 -0.2 3,507,949 - Pf1N1B4_3121 0.21 +1.6 3,507,949 - Pf1N1B4_3121 0.21 +1.2 3,507,949 - Pf1N1B4_3121 0.21 -0.4 3,507,949 - Pf1N1B4_3121 0.21 -1.6 3,507,949 - Pf1N1B4_3121 0.21 -0.6 3,508,100 - Pf1N1B4_3121 0.47 -0.7 3,508,100 - Pf1N1B4_3121 0.47 +0.4 3,508,137 + Pf1N1B4_3121 0.53 +1.4 3,508,152 + Pf1N1B4_3121 0.56 -0.6 3,508,153 - Pf1N1B4_3121 0.56 -0.6 3,508,399 + -0.7 3,508,399 + -0.0 3,508,587 - +0.2 3,508,639 + -1.2 3,508,639 + -0.3 3,508,639 + -1.2 3,508,640 - +0.9 3,508,640 - -0.3 3,508,813 + Pf1N1B4_3123 0.15 +0.7 3,508,813 + Pf1N1B4_3123 0.15 -0.2 3,508,813 + +1.1 3,508,813 + Pf1N1B4_3123 0.15 +1.0 3,508,813 + Pf1N1B4_3123 0.15 +0.7 3,508,813 + Pf1N1B4_3123 0.15 -1.1 3,508,814 - Pf1N1B4_3123 0.15 -0.3 3,508,814 - Pf1N1B4_3123 0.15 -0.4 3,508,945 + Pf1N1B4_3123 0.24 -0.9 3,508,945 + -0.7 3,508,945 + Pf1N1B4_3123 0.24 +0.3 3,509,374 + Pf1N1B4_3123 0.53 +1.2 3,509,374 + Pf1N1B4_3123 0.53 +0.8 3,509,374 + Pf1N1B4_3123 0.53 +1.3
Or see this region's nucleotide sequence