Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2936 and Pf1N1B4_2937 are separated by 150 nucleotides Pf1N1B4_2937 and Pf1N1B4_2938 are separated by 63 nucleotides Pf1N1B4_2938 and Pf1N1B4_2939 are separated by 172 nucleotides
Pf1N1B4_2936 - Putative sulfate permease, at 3,292,455 to 3,294,176
_2936
Pf1N1B4_2937 - FIG00953775: hypothetical protein, at 3,294,327 to 3,294,812
_2937
Pf1N1B4_2938 - Aminopeptidase N, at 3,294,876 to 3,295,163
_2938
Pf1N1B4_2939 - Malate:quinone oxidoreductase (EC 1.1.5.4), at 3,295,336 to 3,296,844
_2939
Position (kb)
3294
3295
3296 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3293.890 kb on + strand, within Pf1N1B4_2936 at 3293.890 kb on + strand, within Pf1N1B4_2936 at 3293.890 kb on + strand, within Pf1N1B4_2936 at 3293.890 kb on + strand at 3293.891 kb on - strand, within Pf1N1B4_2936 at 3293.891 kb on - strand, within Pf1N1B4_2936 at 3293.914 kb on - strand, within Pf1N1B4_2936 at 3293.914 kb on - strand, within Pf1N1B4_2936 at 3293.914 kb on - strand, within Pf1N1B4_2936 at 3293.914 kb on - strand, within Pf1N1B4_2936 at 3293.999 kb on + strand, within Pf1N1B4_2936 at 3293.999 kb on + strand, within Pf1N1B4_2936 at 3294.000 kb on - strand, within Pf1N1B4_2936 at 3294.000 kb on - strand at 3294.002 kb on - strand, within Pf1N1B4_2936 at 3294.002 kb on - strand, within Pf1N1B4_2936 at 3294.002 kb on - strand, within Pf1N1B4_2936 at 3294.116 kb on + strand at 3294.117 kb on - strand at 3294.117 kb on - strand at 3294.256 kb on - strand at 3294.297 kb on - strand at 3294.297 kb on - strand at 3294.297 kb on - strand at 3294.651 kb on + strand, within Pf1N1B4_2937 at 3294.651 kb on + strand, within Pf1N1B4_2937 at 3294.651 kb on + strand, within Pf1N1B4_2937 at 3294.652 kb on - strand, within Pf1N1B4_2937 at 3294.652 kb on - strand, within Pf1N1B4_2937 at 3294.685 kb on - strand, within Pf1N1B4_2937 at 3294.685 kb on - strand, within Pf1N1B4_2937 at 3294.738 kb on + strand, within Pf1N1B4_2937 at 3294.738 kb on + strand, within Pf1N1B4_2937 at 3294.738 kb on + strand, within Pf1N1B4_2937 at 3294.739 kb on - strand, within Pf1N1B4_2937 at 3294.739 kb on - strand, within Pf1N1B4_2937 at 3294.739 kb on - strand, within Pf1N1B4_2937 at 3294.756 kb on + strand, within Pf1N1B4_2937 at 3294.757 kb on - strand, within Pf1N1B4_2937 at 3294.770 kb on + strand at 3294.770 kb on + strand at 3294.771 kb on - strand at 3294.771 kb on - strand at 3294.887 kb on + strand at 3294.888 kb on - strand at 3294.888 kb on - strand at 3295.131 kb on + strand, within Pf1N1B4_2938 at 3295.131 kb on + strand, within Pf1N1B4_2938 at 3295.161 kb on + strand at 3295.216 kb on + strand at 3295.222 kb on + strand at 3295.222 kb on + strand at 3295.222 kb on + strand at 3295.222 kb on + strand at 3295.222 kb on + strand at 3295.222 kb on + strand at 3295.223 kb on - strand at 3295.233 kb on + strand at 3295.234 kb on - strand at 3295.234 kb on - strand at 3295.239 kb on + strand at 3295.240 kb on - strand at 3295.240 kb on - strand at 3295.240 kb on - strand at 3295.240 kb on - strand at 3295.324 kb on + strand at 3295.324 kb on + strand at 3295.325 kb on - strand at 3295.325 kb on - strand at 3295.325 kb on - strand at 3295.343 kb on + strand at 3295.343 kb on + strand at 3295.343 kb on + strand at 3295.710 kb on - strand, within Pf1N1B4_2939 at 3295.769 kb on + strand, within Pf1N1B4_2939 at 3295.770 kb on - strand, within Pf1N1B4_2939 at 3295.892 kb on + strand, within Pf1N1B4_2939 at 3295.985 kb on + strand, within Pf1N1B4_2939 at 3295.985 kb on + strand, within Pf1N1B4_2939 at 3295.985 kb on + strand, within Pf1N1B4_2939 at 3295.986 kb on - strand, within Pf1N1B4_2939
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,293,890 + Pf1N1B4_2936 0.83 -1.0 3,293,890 + Pf1N1B4_2936 0.83 -1.7 3,293,890 + Pf1N1B4_2936 0.83 -3.5 3,293,890 + -0.0 3,293,891 - Pf1N1B4_2936 0.83 -0.6 3,293,891 - Pf1N1B4_2936 0.83 -2.2 3,293,914 - Pf1N1B4_2936 0.85 -2.6 3,293,914 - Pf1N1B4_2936 0.85 -0.0 3,293,914 - Pf1N1B4_2936 0.85 -2.1 3,293,914 - Pf1N1B4_2936 0.85 -2.8 3,293,999 + Pf1N1B4_2936 0.90 -1.2 3,293,999 + Pf1N1B4_2936 0.90 -2.0 3,294,000 - Pf1N1B4_2936 0.90 -1.8 3,294,000 - -0.8 3,294,002 - Pf1N1B4_2936 0.90 -3.0 3,294,002 - Pf1N1B4_2936 0.90 -1.6 3,294,002 - Pf1N1B4_2936 0.90 -2.2 3,294,116 + -1.6 3,294,117 - -2.1 3,294,117 - -0.4 3,294,256 - +1.2 3,294,297 - -0.4 3,294,297 - +0.3 3,294,297 - -0.6 3,294,651 + Pf1N1B4_2937 0.67 -0.4 3,294,651 + Pf1N1B4_2937 0.67 -1.9 3,294,651 + Pf1N1B4_2937 0.67 -0.4 3,294,652 - Pf1N1B4_2937 0.67 +1.8 3,294,652 - Pf1N1B4_2937 0.67 +0.9 3,294,685 - Pf1N1B4_2937 0.74 +2.6 3,294,685 - Pf1N1B4_2937 0.74 -0.8 3,294,738 + Pf1N1B4_2937 0.85 -0.1 3,294,738 + Pf1N1B4_2937 0.85 -0.0 3,294,738 + Pf1N1B4_2937 0.85 -0.0 3,294,739 - Pf1N1B4_2937 0.85 +1.6 3,294,739 - Pf1N1B4_2937 0.85 -0.4 3,294,739 - Pf1N1B4_2937 0.85 -0.8 3,294,756 + Pf1N1B4_2937 0.88 -0.8 3,294,757 - Pf1N1B4_2937 0.88 +1.0 3,294,770 + -2.1 3,294,770 + -0.2 3,294,771 - +2.2 3,294,771 - +0.8 3,294,887 + -1.4 3,294,888 - +0.6 3,294,888 - +0.7 3,295,131 + Pf1N1B4_2938 0.89 -0.8 3,295,131 + Pf1N1B4_2938 0.89 -0.1 3,295,161 + +0.2 3,295,216 + +0.8 3,295,222 + +1.2 3,295,222 + -1.1 3,295,222 + +1.1 3,295,222 + -0.4 3,295,222 + +0.6 3,295,222 + +1.6 3,295,223 - +0.1 3,295,233 + -0.7 3,295,234 - +0.6 3,295,234 - +0.1 3,295,239 + -1.4 3,295,240 - -1.8 3,295,240 - -0.2 3,295,240 - -1.6 3,295,240 - +1.3 3,295,324 + +0.6 3,295,324 + -0.3 3,295,325 - +0.3 3,295,325 - -0.5 3,295,325 - -1.7 3,295,343 + -0.9 3,295,343 + +0.6 3,295,343 + +1.1 3,295,710 - Pf1N1B4_2939 0.25 -0.0 3,295,769 + Pf1N1B4_2939 0.29 -0.4 3,295,770 - Pf1N1B4_2939 0.29 -0.4 3,295,892 + Pf1N1B4_2939 0.37 -1.0 3,295,985 + Pf1N1B4_2939 0.43 +1.6 3,295,985 + Pf1N1B4_2939 0.43 +1.6 3,295,985 + Pf1N1B4_2939 0.43 -0.0 3,295,986 - Pf1N1B4_2939 0.43 -0.4
Or see this region's nucleotide sequence