Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2803

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2801 and Pf1N1B4_2802 are separated by 184 nucleotidesPf1N1B4_2802 and Pf1N1B4_2803 are separated by 252 nucleotidesPf1N1B4_2803 and Pf1N1B4_2804 are separated by 261 nucleotides Pf1N1B4_2801 - Conserved uncharacterized protein CreA, at 3,144,458 to 3,144,922 _2801 Pf1N1B4_2802 - SSU ribosomal protein S20p, at 3,145,107 to 3,145,385 _2802 Pf1N1B4_2803 - Proposed peptidoglycan lipid II flippase MurJ, at 3,145,638 to 3,147,176 _2803 Pf1N1B4_2804 - Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2), at 3,147,438 to 3,148,256 _2804 Position (kb) 3145 3146 3147 3148Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3144.648 kb on + strand, within Pf1N1B4_2801at 3144.660 kb on + strand, within Pf1N1B4_2801at 3144.660 kb on + strand, within Pf1N1B4_2801at 3144.661 kb on - strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.691 kb on + strand, within Pf1N1B4_2801at 3144.823 kb on + strand, within Pf1N1B4_2801at 3144.823 kb on + strand, within Pf1N1B4_2801at 3144.824 kb on - strand, within Pf1N1B4_2801at 3144.833 kb on + strand, within Pf1N1B4_2801at 3144.833 kb on + strand, within Pf1N1B4_2801at 3144.833 kb on + strand, within Pf1N1B4_2801at 3144.833 kb on + strand, within Pf1N1B4_2801at 3144.834 kb on - strand, within Pf1N1B4_2801at 3144.834 kb on - strand, within Pf1N1B4_2801at 3144.842 kb on + strand, within Pf1N1B4_2801at 3144.842 kb on + strand, within Pf1N1B4_2801at 3144.842 kb on + strand, within Pf1N1B4_2801at 3144.925 kb on + strandat 3144.925 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.985 kb on + strandat 3144.986 kb on - strandat 3144.986 kb on - strandat 3144.986 kb on - strandat 3145.015 kb on - strandat 3145.500 kb on - strandat 3147.274 kb on + strandat 3147.287 kb on + strandat 3147.287 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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3,144,648 + Pf1N1B4_2801 0.41 -0.0
3,144,660 + Pf1N1B4_2801 0.43 +1.1
3,144,660 + Pf1N1B4_2801 0.43 -1.6
3,144,661 - Pf1N1B4_2801 0.44 -1.4
3,144,691 + Pf1N1B4_2801 0.50 +0.2
3,144,691 + Pf1N1B4_2801 0.50 +0.4
3,144,691 + Pf1N1B4_2801 0.50 -0.8
3,144,691 + Pf1N1B4_2801 0.50 -1.8
3,144,691 + Pf1N1B4_2801 0.50 -1.6
3,144,691 + Pf1N1B4_2801 0.50 +2.6
3,144,691 + Pf1N1B4_2801 0.50 -0.8
3,144,691 + Pf1N1B4_2801 0.50 -0.3
3,144,691 + Pf1N1B4_2801 0.50 +1.2
3,144,691 + Pf1N1B4_2801 0.50 -0.0
3,144,823 + Pf1N1B4_2801 0.78 -0.4
3,144,823 + Pf1N1B4_2801 0.78 -1.5
3,144,824 - Pf1N1B4_2801 0.79 -0.6
3,144,833 + Pf1N1B4_2801 0.81 +0.1
3,144,833 + Pf1N1B4_2801 0.81 -0.4
3,144,833 + Pf1N1B4_2801 0.81 +0.2
3,144,833 + Pf1N1B4_2801 0.81 +0.6
3,144,834 - Pf1N1B4_2801 0.81 -0.0
3,144,834 - Pf1N1B4_2801 0.81 +0.7
3,144,842 + Pf1N1B4_2801 0.83 -0.0
3,144,842 + Pf1N1B4_2801 0.83 -1.1
3,144,842 + Pf1N1B4_2801 0.83 +0.1
3,144,925 + +0.9
3,144,925 + -0.4
3,144,985 + -0.0
3,144,985 + -0.8
3,144,985 + -0.6
3,144,985 + -1.0
3,144,985 + -1.0
3,144,985 + -0.8
3,144,985 + -0.2
3,144,985 + -1.1
3,144,985 + -2.7
3,144,985 + +0.2
3,144,985 + -0.3
3,144,985 + -1.2
3,144,985 + -1.2
3,144,985 + -0.6
3,144,985 + +0.7
3,144,985 + -3.2
3,144,985 + -2.4
3,144,985 + -0.3
3,144,985 + -0.7
3,144,985 + +0.2
3,144,985 + -0.6
3,144,985 + +1.0
3,144,986 - -0.0
3,144,986 - +0.8
3,144,986 - -0.5
3,145,015 - -0.8
3,145,500 - +0.4
3,147,274 + +1.9
3,147,287 + +1.1
3,147,287 + +0.6

Or see this region's nucleotide sequence