Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2801 and Pf1N1B4_2802 are separated by 184 nucleotides Pf1N1B4_2802 and Pf1N1B4_2803 are separated by 252 nucleotides Pf1N1B4_2803 and Pf1N1B4_2804 are separated by 261 nucleotides
Pf1N1B4_2801 - Conserved uncharacterized protein CreA, at 3,144,458 to 3,144,922
_2801
Pf1N1B4_2802 - SSU ribosomal protein S20p, at 3,145,107 to 3,145,385
_2802
Pf1N1B4_2803 - Proposed peptidoglycan lipid II flippase MurJ, at 3,145,638 to 3,147,176
_2803
Pf1N1B4_2804 - Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2), at 3,147,438 to 3,148,256
_2804
Position (kb)
3145
3146
3147
3148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3144.648 kb on + strand, within Pf1N1B4_2801 at 3144.660 kb on + strand, within Pf1N1B4_2801 at 3144.660 kb on + strand, within Pf1N1B4_2801 at 3144.661 kb on - strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.823 kb on + strand, within Pf1N1B4_2801 at 3144.823 kb on + strand, within Pf1N1B4_2801 at 3144.824 kb on - strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.834 kb on - strand, within Pf1N1B4_2801 at 3144.834 kb on - strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.925 kb on + strand at 3144.925 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.986 kb on - strand at 3144.986 kb on - strand at 3144.986 kb on - strand at 3145.015 kb on - strand at 3145.500 kb on - strand at 3147.274 kb on + strand at 3147.287 kb on + strand at 3147.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,144,648 + Pf1N1B4_2801 0.41 -0.0 3,144,660 + Pf1N1B4_2801 0.43 +1.1 3,144,660 + Pf1N1B4_2801 0.43 -1.6 3,144,661 - Pf1N1B4_2801 0.44 -1.4 3,144,691 + Pf1N1B4_2801 0.50 +0.2 3,144,691 + Pf1N1B4_2801 0.50 +0.4 3,144,691 + Pf1N1B4_2801 0.50 -0.8 3,144,691 + Pf1N1B4_2801 0.50 -1.8 3,144,691 + Pf1N1B4_2801 0.50 -1.6 3,144,691 + Pf1N1B4_2801 0.50 +2.6 3,144,691 + Pf1N1B4_2801 0.50 -0.8 3,144,691 + Pf1N1B4_2801 0.50 -0.3 3,144,691 + Pf1N1B4_2801 0.50 +1.2 3,144,691 + Pf1N1B4_2801 0.50 -0.0 3,144,823 + Pf1N1B4_2801 0.78 -0.4 3,144,823 + Pf1N1B4_2801 0.78 -1.5 3,144,824 - Pf1N1B4_2801 0.79 -0.6 3,144,833 + Pf1N1B4_2801 0.81 +0.1 3,144,833 + Pf1N1B4_2801 0.81 -0.4 3,144,833 + Pf1N1B4_2801 0.81 +0.2 3,144,833 + Pf1N1B4_2801 0.81 +0.6 3,144,834 - Pf1N1B4_2801 0.81 -0.0 3,144,834 - Pf1N1B4_2801 0.81 +0.7 3,144,842 + Pf1N1B4_2801 0.83 -0.0 3,144,842 + Pf1N1B4_2801 0.83 -1.1 3,144,842 + Pf1N1B4_2801 0.83 +0.1 3,144,925 + +0.9 3,144,925 + -0.4 3,144,985 + -0.0 3,144,985 + -0.8 3,144,985 + -0.6 3,144,985 + -1.0 3,144,985 + -1.0 3,144,985 + -0.8 3,144,985 + -0.2 3,144,985 + -1.1 3,144,985 + -2.7 3,144,985 + +0.2 3,144,985 + -0.3 3,144,985 + -1.2 3,144,985 + -1.2 3,144,985 + -0.6 3,144,985 + +0.7 3,144,985 + -3.2 3,144,985 + -2.4 3,144,985 + -0.3 3,144,985 + -0.7 3,144,985 + +0.2 3,144,985 + -0.6 3,144,985 + +1.0 3,144,986 - -0.0 3,144,986 - +0.8 3,144,986 - -0.5 3,145,015 - -0.8 3,145,500 - +0.4 3,147,274 + +1.9 3,147,287 + +1.1 3,147,287 + +0.6
Or see this region's nucleotide sequence