Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2661 and Pf1N1B4_2662 are separated by 91 nucleotides Pf1N1B4_2662 and Pf1N1B4_2663 overlap by 4 nucleotides Pf1N1B4_2663 and Pf1N1B4_2664 are separated by 273 nucleotides
Pf1N1B4_2661 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 2,997,228 to 2,999,126
_2661
Pf1N1B4_2662 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29), at 2,999,218 to 3,000,105
_2662
Pf1N1B4_2663 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 3,000,102 to 3,000,344
_2663
Pf1N1B4_2664 - Fic family protein, at 3,000,618 to 3,001,763
_2664
Position (kb)
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2999.140 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 3000.368 kb on - strand at 3000.409 kb on + strand at 3000.409 kb on + strand at 3000.409 kb on + strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.420 kb on + strand at 3000.619 kb on + strand at 3000.620 kb on - strand at 3000.761 kb on - strand, within Pf1N1B4_2664 at 3000.822 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.974 kb on + strand at 3000.974 kb on + strand, within Pf1N1B4_2664 at 3000.975 kb on - strand at 3001.046 kb on - strand, within Pf1N1B4_2664 at 3001.046 kb on - strand at 3001.083 kb on + strand, within Pf1N1B4_2664
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,999,140 - +0.6 2,999,142 - -1.0 2,999,142 - +0.3 2,999,142 - +0.6 2,999,142 - -0.9 2,999,142 - +0.3 2,999,142 - -0.4 2,999,142 - +0.3 2,999,142 - +2.0 2,999,142 - +0.8 2,999,142 - +0.2 2,999,142 - -0.2 2,999,142 - +0.6 2,999,142 - -0.4 2,999,142 - +1.0 2,999,142 - -0.5 2,999,142 - +0.6 2,999,142 - +0.7 2,999,142 - -0.0 2,999,142 - -1.1 2,999,142 - +0.1 2,999,142 - -1.6 2,999,142 - +1.3 2,999,142 - +0.6 2,999,142 - -0.0 2,999,146 - -0.2 2,999,146 - +0.7 2,999,146 - +1.0 2,999,146 - +0.9 2,999,146 - -1.4 2,999,146 - +1.1 2,999,148 - +0.2 2,999,148 - +2.6 2,999,148 - -0.2 2,999,148 - +1.1 2,999,148 - +1.3 2,999,148 - +0.2 2,999,148 - -0.4 2,999,148 - +0.7 2,999,148 - -1.6 2,999,148 - -0.2 2,999,148 - +0.8 2,999,148 - -0.2 2,999,148 - -0.7 2,999,148 - +0.1 3,000,368 - -0.8 3,000,409 + -0.0 3,000,409 + -0.0 3,000,409 + -1.3 3,000,410 - +0.6 3,000,410 - +3.1 3,000,410 - +0.5 3,000,410 - +1.8 3,000,410 - -0.2 3,000,410 - +1.2 3,000,410 - -1.0 3,000,420 + -0.0 3,000,619 + +4.1 3,000,620 - -0.0 3,000,761 - Pf1N1B4_2664 0.12 -0.4 3,000,822 + Pf1N1B4_2664 0.18 +1.0 3,000,923 + Pf1N1B4_2664 0.27 -1.7 3,000,923 + Pf1N1B4_2664 0.27 -1.2 3,000,923 + Pf1N1B4_2664 0.27 -0.8 3,000,923 + Pf1N1B4_2664 0.27 -1.9 3,000,923 + Pf1N1B4_2664 0.27 -0.0 3,000,974 + -1.9 3,000,974 + Pf1N1B4_2664 0.31 +0.9 3,000,975 - +1.6 3,001,046 - Pf1N1B4_2664 0.37 +0.6 3,001,046 - -0.2 3,001,083 + Pf1N1B4_2664 0.41 +0.3
Or see this region's nucleotide sequence