Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2557 and Pf1N1B4_2558 are separated by 33 nucleotides Pf1N1B4_2558 and Pf1N1B4_2559 are separated by 135 nucleotides
Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290
_2557
Pf1N1B4_2558 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 2,900,324 to 2,900,755
_2558
Pf1N1B4_2559 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,900,891 to 2,901,925
_2559
Position (kb)
2900
2901 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2899.587 kb on - strand, within Pf1N1B4_2557 at 2899.607 kb on - strand, within Pf1N1B4_2557 at 2899.648 kb on + strand, within Pf1N1B4_2557 at 2899.649 kb on - strand, within Pf1N1B4_2557 at 2899.649 kb on - strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.651 kb on - strand, within Pf1N1B4_2557 at 2899.651 kb on - strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2900.000 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand at 2900.180 kb on + strand, within Pf1N1B4_2557 at 2900.224 kb on + strand at 2900.225 kb on - strand at 2900.243 kb on + strand at 2900.244 kb on - strand at 2900.244 kb on - strand at 2900.325 kb on + strand at 2900.325 kb on + strand at 2900.326 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.792 kb on + strand at 2900.792 kb on + strand at 2900.849 kb on + strand at 2900.849 kb on + strand at 2900.890 kb on + strand at 2900.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,899,587 - Pf1N1B4_2557 0.27 -1.1 2,899,607 - Pf1N1B4_2557 0.29 -1.0 2,899,648 + Pf1N1B4_2557 0.34 +0.8 2,899,649 - Pf1N1B4_2557 0.34 -0.3 2,899,649 - Pf1N1B4_2557 0.34 -0.8 2,899,650 + Pf1N1B4_2557 0.34 -0.4 2,899,650 + Pf1N1B4_2557 0.34 -0.9 2,899,650 + Pf1N1B4_2557 0.34 +1.2 2,899,650 + Pf1N1B4_2557 0.34 +0.1 2,899,651 - Pf1N1B4_2557 0.34 -1.0 2,899,651 - Pf1N1B4_2557 0.34 -1.0 2,899,941 + Pf1N1B4_2557 0.64 +0.2 2,899,941 + Pf1N1B4_2557 0.64 -0.0 2,899,941 + Pf1N1B4_2557 0.64 +0.6 2,900,000 + Pf1N1B4_2557 0.70 +0.7 2,900,076 + Pf1N1B4_2557 0.78 -0.0 2,900,076 + +0.6 2,900,180 + Pf1N1B4_2557 0.89 -0.4 2,900,224 + -1.6 2,900,225 - -0.7 2,900,243 + +0.1 2,900,244 - +1.1 2,900,244 - -0.3 2,900,325 + -0.4 2,900,325 + -0.4 2,900,326 - -0.3 2,900,760 - -1.8 2,900,760 - +0.3 2,900,760 - -0.0 2,900,789 + -0.4 2,900,789 + -0.0 2,900,789 + +0.6 2,900,789 + -0.8 2,900,789 + -0.4 2,900,789 + +0.3 2,900,789 + -0.4 2,900,789 + +0.8 2,900,789 + -1.4 2,900,789 + -1.6 2,900,789 + -2.3 2,900,789 + +1.6 2,900,789 + +1.1 2,900,789 + +0.9 2,900,789 + -1.6 2,900,789 + -0.4 2,900,790 - -2.3 2,900,790 - -0.0 2,900,790 - -2.3 2,900,790 - -0.8 2,900,790 - -0.3 2,900,792 + -0.0 2,900,792 + +1.6 2,900,849 + -2.8 2,900,849 + +0.8 2,900,890 + +1.6 2,900,890 + +1.6
Or see this region's nucleotide sequence