Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_244 and Pf1N1B4_245 are separated by 219 nucleotides Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides Pf1N1B4_247 and Pf1N1B4_248 are separated by 123 nucleotides
Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925
_243
Pf1N1B4_244 - hypothetical protein, at 302,821 to 302,946
_244
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Position (kb) 303
304
305 Strain fitness (log2 ratio) -1
0
1
2 at 303.008 kb on + strand at 303.009 kb on - strand at 303.079 kb on + strand at 303.080 kb on - strand at 303.104 kb on + strand at 303.105 kb on - strand at 303.128 kb on + strand at 303.128 kb on + strand at 303.129 kb on - strand at 303.160 kb on + strand at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246 at 304.204 kb on + strand at 304.247 kb on + strand at 304.315 kb on + strand at 304.315 kb on + strand at 304.316 kb on - strand at 304.318 kb on + strand at 304.318 kb on + strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248 at 304.981 kb on + strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 305.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 303,008 + +0.6 303,009 - -0.7 303,079 + -0.0 303,080 - -0.3 303,104 + -0.6 303,105 - -0.1 303,128 + +1.1 303,128 + -1.3 303,129 - +0.1 303,160 + +2.0 303,397 + Pf1N1B4_245 0.73 +0.4 303,397 + Pf1N1B4_245 0.73 -0.3 303,482 - -0.4 303,482 - -0.9 303,716 + Pf1N1B4_246 0.60 -0.8 303,752 + Pf1N1B4_246 0.69 +0.7 303,752 + Pf1N1B4_246 0.69 -1.1 303,752 + Pf1N1B4_246 0.69 +0.3 303,752 + Pf1N1B4_246 0.69 -1.3 303,752 + Pf1N1B4_246 0.69 +0.6 303,752 + Pf1N1B4_246 0.69 +0.4 303,752 + Pf1N1B4_246 0.69 -0.1 303,752 + Pf1N1B4_246 0.69 +1.6 303,752 + Pf1N1B4_246 0.69 -0.4 303,752 + Pf1N1B4_246 0.69 +0.3 303,753 - Pf1N1B4_246 0.69 -1.2 304,204 + -0.4 304,247 + +0.4 304,315 + +0.3 304,315 + -0.2 304,316 - +0.2 304,318 + -0.2 304,318 + -0.1 304,319 - -1.6 304,319 - -0.0 304,319 - -1.4 304,574 + Pf1N1B4_248 0.21 +0.4 304,575 - Pf1N1B4_248 0.21 +0.0 304,981 + Pf1N1B4_248 0.56 +0.1 304,986 - Pf1N1B4_248 0.56 -0.1 304,986 - Pf1N1B4_248 0.56 -0.0 304,986 - Pf1N1B4_248 0.56 +0.3 305,072 - +1.1
Or see this region's nucleotide sequence