Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2349 and Pf1N1B4_2350 overlap by 1 nucleotides Pf1N1B4_2350 and Pf1N1B4_2351 are separated by 190 nucleotides Pf1N1B4_2351 and Pf1N1B4_2352 are separated by 151 nucleotides
Pf1N1B4_2349 - Putative Holliday junction resolvase YggF, at 2,664,044 to 2,664,481
_2349
Pf1N1B4_2350 - UPF0301 protein YqgE, at 2,664,481 to 2,665,053
_2350
Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098
_2351
Pf1N1B4_2352 - Glutathione synthetase (EC 6.3.2.3), at 2,666,250 to 2,667,221
_2352
Position (kb)
2665
2666
2667 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2665.061 kb on - strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.173 kb on - strand at 2665.173 kb on - strand at 2665.249 kb on + strand at 2665.249 kb on + strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2666.159 kb on + strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.214 kb on + strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.237 kb on + strand at 2666.238 kb on - strand at 2666.238 kb on - strand at 2666.292 kb on + strand at 2666.293 kb on - strand at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.398 kb on - strand, within Pf1N1B4_2352 at 2666.621 kb on - strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.929 kb on + strand, within Pf1N1B4_2352 at 2666.930 kb on - strand, within Pf1N1B4_2352 at 2667.088 kb on + strand, within Pf1N1B4_2352 at 2667.089 kb on - strand, within Pf1N1B4_2352
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,664,554 - Pf1N1B4_2350 0.13 +1.0 2,664,554 - Pf1N1B4_2350 0.13 -1.6 2,664,963 - Pf1N1B4_2350 0.84 -0.6 2,664,963 - Pf1N1B4_2350 0.84 -1.6 2,664,963 - Pf1N1B4_2350 0.84 +2.7 2,664,963 - Pf1N1B4_2350 0.84 -0.6 2,664,963 - Pf1N1B4_2350 0.84 -0.0 2,664,963 - Pf1N1B4_2350 0.84 +0.6 2,664,963 - Pf1N1B4_2350 0.84 -0.4 2,664,963 - Pf1N1B4_2350 0.84 -1.3 2,665,061 - -0.9 2,665,172 + +0.6 2,665,172 + -0.0 2,665,172 + -1.7 2,665,173 - +0.2 2,665,173 - -0.2 2,665,249 + -0.2 2,665,249 + +2.6 2,665,250 - -1.6 2,665,250 - +2.9 2,666,159 + -1.2 2,666,160 - -1.5 2,666,160 - +0.4 2,666,160 - +0.4 2,666,214 + -0.6 2,666,215 - -0.7 2,666,215 - +0.6 2,666,215 - +1.5 2,666,215 - -0.1 2,666,215 - -0.5 2,666,237 + +0.4 2,666,238 - +1.2 2,666,238 - -1.8 2,666,292 + -0.7 2,666,293 - -0.7 2,666,397 + Pf1N1B4_2352 0.15 +2.2 2,666,397 + Pf1N1B4_2352 0.15 -0.0 2,666,398 - Pf1N1B4_2352 0.15 -1.0 2,666,621 - Pf1N1B4_2352 0.38 +1.6 2,666,920 + Pf1N1B4_2352 0.69 +1.6 2,666,920 + Pf1N1B4_2352 0.69 -1.2 2,666,920 + Pf1N1B4_2352 0.69 -1.0 2,666,921 - Pf1N1B4_2352 0.69 -0.7 2,666,921 - Pf1N1B4_2352 0.69 -0.0 2,666,921 - Pf1N1B4_2352 0.69 +0.6 2,666,921 - Pf1N1B4_2352 0.69 +0.6 2,666,929 + Pf1N1B4_2352 0.70 +1.6 2,666,930 - Pf1N1B4_2352 0.70 -0.0 2,667,088 + Pf1N1B4_2352 0.86 +1.0 2,667,089 - Pf1N1B4_2352 0.86 -0.4
Or see this region's nucleotide sequence