Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2351

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2349 and Pf1N1B4_2350 overlap by 1 nucleotidesPf1N1B4_2350 and Pf1N1B4_2351 are separated by 190 nucleotidesPf1N1B4_2351 and Pf1N1B4_2352 are separated by 151 nucleotides Pf1N1B4_2349 - Putative Holliday junction resolvase YggF, at 2,664,044 to 2,664,481 _2349 Pf1N1B4_2350 - UPF0301 protein YqgE, at 2,664,481 to 2,665,053 _2350 Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098 _2351 Pf1N1B4_2352 - Glutathione synthetase (EC 6.3.2.3), at 2,666,250 to 2,667,221 _2352 Position (kb) 2665 2666 2667Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2664.554 kb on - strand, within Pf1N1B4_2350at 2664.554 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2665.061 kb on - strandat 2665.172 kb on + strandat 2665.172 kb on + strandat 2665.172 kb on + strandat 2665.173 kb on - strandat 2665.173 kb on - strandat 2665.249 kb on + strandat 2665.249 kb on + strandat 2665.250 kb on - strandat 2665.250 kb on - strandat 2666.159 kb on + strandat 2666.160 kb on - strandat 2666.160 kb on - strandat 2666.160 kb on - strandat 2666.214 kb on + strandat 2666.215 kb on - strandat 2666.215 kb on - strandat 2666.215 kb on - strandat 2666.215 kb on - strandat 2666.215 kb on - strandat 2666.237 kb on + strandat 2666.238 kb on - strandat 2666.238 kb on - strandat 2666.292 kb on + strandat 2666.293 kb on - strandat 2666.397 kb on + strand, within Pf1N1B4_2352at 2666.397 kb on + strand, within Pf1N1B4_2352at 2666.398 kb on - strand, within Pf1N1B4_2352at 2666.621 kb on - strand, within Pf1N1B4_2352at 2666.920 kb on + strand, within Pf1N1B4_2352at 2666.920 kb on + strand, within Pf1N1B4_2352at 2666.920 kb on + strand, within Pf1N1B4_2352at 2666.921 kb on - strand, within Pf1N1B4_2352at 2666.921 kb on - strand, within Pf1N1B4_2352at 2666.921 kb on - strand, within Pf1N1B4_2352at 2666.921 kb on - strand, within Pf1N1B4_2352at 2666.929 kb on + strand, within Pf1N1B4_2352at 2666.930 kb on - strand, within Pf1N1B4_2352at 2667.088 kb on + strand, within Pf1N1B4_2352at 2667.089 kb on - strand, within Pf1N1B4_2352

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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2,664,554 - Pf1N1B4_2350 0.13 +1.0
2,664,554 - Pf1N1B4_2350 0.13 -1.6
2,664,963 - Pf1N1B4_2350 0.84 -0.6
2,664,963 - Pf1N1B4_2350 0.84 -1.6
2,664,963 - Pf1N1B4_2350 0.84 +2.7
2,664,963 - Pf1N1B4_2350 0.84 -0.6
2,664,963 - Pf1N1B4_2350 0.84 -0.0
2,664,963 - Pf1N1B4_2350 0.84 +0.6
2,664,963 - Pf1N1B4_2350 0.84 -0.4
2,664,963 - Pf1N1B4_2350 0.84 -1.3
2,665,061 - -0.9
2,665,172 + +0.6
2,665,172 + -0.0
2,665,172 + -1.7
2,665,173 - +0.2
2,665,173 - -0.2
2,665,249 + -0.2
2,665,249 + +2.6
2,665,250 - -1.6
2,665,250 - +2.9
2,666,159 + -1.2
2,666,160 - -1.5
2,666,160 - +0.4
2,666,160 - +0.4
2,666,214 + -0.6
2,666,215 - -0.7
2,666,215 - +0.6
2,666,215 - +1.5
2,666,215 - -0.1
2,666,215 - -0.5
2,666,237 + +0.4
2,666,238 - +1.2
2,666,238 - -1.8
2,666,292 + -0.7
2,666,293 - -0.7
2,666,397 + Pf1N1B4_2352 0.15 +2.2
2,666,397 + Pf1N1B4_2352 0.15 -0.0
2,666,398 - Pf1N1B4_2352 0.15 -1.0
2,666,621 - Pf1N1B4_2352 0.38 +1.6
2,666,920 + Pf1N1B4_2352 0.69 +1.6
2,666,920 + Pf1N1B4_2352 0.69 -1.2
2,666,920 + Pf1N1B4_2352 0.69 -1.0
2,666,921 - Pf1N1B4_2352 0.69 -0.7
2,666,921 - Pf1N1B4_2352 0.69 -0.0
2,666,921 - Pf1N1B4_2352 0.69 +0.6
2,666,921 - Pf1N1B4_2352 0.69 +0.6
2,666,929 + Pf1N1B4_2352 0.70 +1.6
2,666,930 - Pf1N1B4_2352 0.70 -0.0
2,667,088 + Pf1N1B4_2352 0.86 +1.0
2,667,089 - Pf1N1B4_2352 0.86 -0.4

Or see this region's nucleotide sequence