Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2144 and Pf1N1B4_2145 overlap by 29 nucleotides Pf1N1B4_2145 and Pf1N1B4_2146 are separated by 97 nucleotides Pf1N1B4_2146 and Pf1N1B4_2147 are separated by 129 nucleotides Pf1N1B4_2147 and Pf1N1B4_2148 are separated by 317 nucleotides
Pf1N1B4_2144 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 2,454,539 to 2,455,843
_2144
Pf1N1B4_2145 - Translation initiation inhibitor, at 2,455,815 to 2,456,168
_2145
Pf1N1B4_2146 - Alanine racemase (EC 5.1.1.1), at 2,456,266 to 2,457,339
_2146
Pf1N1B4_2147 - Putrescine utilization regulator, at 2,457,469 to 2,458,017
_2147
Pf1N1B4_2148 - Cytochrome c5, at 2,458,335 to 2,458,613
_2148
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.349 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.774 kb on + strand at 2455.774 kb on + strand at 2455.874 kb on + strand, within Pf1N1B4_2145 at 2456.013 kb on + strand, within Pf1N1B4_2145 at 2456.251 kb on + strand at 2457.325 kb on + strand at 2457.326 kb on - strand at 2457.326 kb on - strand at 2457.326 kb on - strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.399 kb on - strand at 2457.402 kb on + strand at 2457.402 kb on + strand at 2457.402 kb on + strand at 2457.403 kb on - strand at 2457.403 kb on - strand at 2457.403 kb on - strand at 2457.433 kb on + strand at 2457.434 kb on - strand at 2457.434 kb on - strand at 2457.434 kb on - strand at 2457.550 kb on - strand, within Pf1N1B4_2147 at 2457.694 kb on + strand, within Pf1N1B4_2147 at 2457.694 kb on + strand, within Pf1N1B4_2147 at 2457.695 kb on - strand, within Pf1N1B4_2147 at 2457.796 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.926 kb on - strand, within Pf1N1B4_2147 at 2457.926 kb on - strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand at 2457.929 kb on - strand, within Pf1N1B4_2147 at 2457.929 kb on - strand, within Pf1N1B4_2147 at 2458.016 kb on - strand at 2458.060 kb on + strand at 2458.060 kb on + strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.119 kb on - strand at 2458.124 kb on + strand at 2458.125 kb on - strand at 2458.125 kb on - strand at 2458.125 kb on - strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.166 kb on - strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.228 kb on + strand at 2458.229 kb on - strand at 2458.274 kb on + strand at 2458.274 kb on + strand at 2458.275 kb on - strand at 2458.280 kb on + strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,455,283 + Pf1N1B4_2144 0.57 +1.0 2,455,283 + Pf1N1B4_2144 0.57 -0.5 2,455,305 - Pf1N1B4_2144 0.59 +0.2 2,455,305 - Pf1N1B4_2144 0.59 +1.6 2,455,349 + Pf1N1B4_2144 0.62 +2.2 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 +1.6 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 +0.3 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 +2.6 2,455,430 + Pf1N1B4_2144 0.68 -1.2 2,455,430 + Pf1N1B4_2144 0.68 -1.0 2,455,430 + Pf1N1B4_2144 0.68 +0.2 2,455,430 + Pf1N1B4_2144 0.68 +1.6 2,455,430 + Pf1N1B4_2144 0.68 +1.6 2,455,430 + Pf1N1B4_2144 0.68 -1.2 2,455,430 + Pf1N1B4_2144 0.68 +1.6 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 +0.6 2,455,430 + Pf1N1B4_2144 0.68 -1.7 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 -0.4 2,455,430 + Pf1N1B4_2144 0.68 -0.7 2,455,430 + Pf1N1B4_2144 0.68 +1.3 2,455,430 + Pf1N1B4_2144 0.68 -1.0 2,455,430 + Pf1N1B4_2144 0.68 +0.0 2,455,431 - Pf1N1B4_2144 0.68 +0.6 2,455,431 - Pf1N1B4_2144 0.68 +0.8 2,455,431 - Pf1N1B4_2144 0.68 -0.4 2,455,431 - Pf1N1B4_2144 0.68 +2.1 2,455,431 - Pf1N1B4_2144 0.68 -0.7 2,455,431 - Pf1N1B4_2144 0.68 -1.6 2,455,432 + Pf1N1B4_2144 0.68 -0.4 2,455,432 + Pf1N1B4_2144 0.68 +0.0 2,455,432 + Pf1N1B4_2144 0.68 +0.6 2,455,432 + Pf1N1B4_2144 0.68 -0.7 2,455,432 + Pf1N1B4_2144 0.68 -0.7 2,455,433 - Pf1N1B4_2144 0.69 +2.6 2,455,433 - Pf1N1B4_2144 0.69 +0.6 2,455,433 - Pf1N1B4_2144 0.69 +1.6 2,455,774 + +0.3 2,455,774 + -0.4 2,455,874 + Pf1N1B4_2145 0.17 -1.7 2,456,013 + Pf1N1B4_2145 0.56 -0.9 2,456,251 + +0.0 2,457,325 + -0.3 2,457,326 - +0.3 2,457,326 - -0.4 2,457,326 - +2.6 2,457,398 + +1.3 2,457,398 + -0.7 2,457,398 + +1.4 2,457,398 + -2.0 2,457,399 - +0.9 2,457,402 + +1.0 2,457,402 + +0.2 2,457,402 + +2.7 2,457,403 - -0.4 2,457,403 - +1.2 2,457,403 - +0.0 2,457,433 + -0.4 2,457,434 - -0.8 2,457,434 - -1.5 2,457,434 - -0.1 2,457,550 - Pf1N1B4_2147 0.15 +0.9 2,457,694 + Pf1N1B4_2147 0.41 +0.0 2,457,694 + Pf1N1B4_2147 0.41 +1.6 2,457,695 - Pf1N1B4_2147 0.41 +1.6 2,457,796 + Pf1N1B4_2147 0.60 +0.9 2,457,925 + Pf1N1B4_2147 0.83 +0.6 2,457,925 + Pf1N1B4_2147 0.83 +0.0 2,457,925 + +1.6 2,457,925 + Pf1N1B4_2147 0.83 +1.2 2,457,925 + Pf1N1B4_2147 0.83 +1.6 2,457,925 + +1.6 2,457,925 + Pf1N1B4_2147 0.83 +1.6 2,457,925 + Pf1N1B4_2147 0.83 +0.9 2,457,926 - Pf1N1B4_2147 0.83 +0.6 2,457,926 - Pf1N1B4_2147 0.83 -1.0 2,457,928 + Pf1N1B4_2147 0.84 -0.2 2,457,928 + Pf1N1B4_2147 0.84 -1.5 2,457,928 + Pf1N1B4_2147 0.84 -0.4 2,457,928 + Pf1N1B4_2147 0.84 -0.4 2,457,928 + Pf1N1B4_2147 0.84 +1.0 2,457,928 + +0.9 2,457,929 - Pf1N1B4_2147 0.84 +0.0 2,457,929 - Pf1N1B4_2147 0.84 +0.6 2,458,016 - +1.9 2,458,060 + +0.8 2,458,060 + +0.5 2,458,061 - -1.6 2,458,061 - -1.0 2,458,061 - -0.7 2,458,061 - +0.3 2,458,061 - +0.3 2,458,061 - -1.2 2,458,061 - +2.6 2,458,119 - -0.8 2,458,124 + +0.6 2,458,125 - +0.3 2,458,125 - +1.1 2,458,125 - +0.1 2,458,126 + +0.1 2,458,126 + +0.3 2,458,126 + -0.4 2,458,126 + +0.0 2,458,126 + -0.2 2,458,126 + -0.2 2,458,127 - -1.2 2,458,127 - +0.7 2,458,127 - +0.6 2,458,127 - +0.3 2,458,127 - +0.2 2,458,127 - -1.5 2,458,127 - -0.1 2,458,127 - -1.6 2,458,127 - -0.3 2,458,127 - -0.1 2,458,127 - -0.1 2,458,127 - +0.9 2,458,127 - -1.0 2,458,127 - -0.6 2,458,127 - +1.0 2,458,127 - -0.7 2,458,127 - -2.7 2,458,127 - -1.6 2,458,127 - -2.1 2,458,127 - -0.2 2,458,127 - -0.3 2,458,127 - -1.7 2,458,127 - +0.3 2,458,127 - -1.1 2,458,127 - +1.3 2,458,127 - -0.6 2,458,127 - +0.6 2,458,127 - -0.5 2,458,127 - -0.4 2,458,127 - -0.4 2,458,127 - +0.0 2,458,166 - -1.9 2,458,183 + -1.5 2,458,183 + +1.6 2,458,183 + -1.3 2,458,183 + -1.0 2,458,183 + -0.7 2,458,183 + -1.0 2,458,184 - +1.9 2,458,184 - -0.1 2,458,184 - +1.4 2,458,184 - -2.3 2,458,184 - +1.6 2,458,184 - +0.9 2,458,184 - -1.1 2,458,184 - -1.6 2,458,184 - +1.6 2,458,184 - -0.9 2,458,195 + +0.0 2,458,195 + -0.3 2,458,195 + -0.3 2,458,195 + -0.4 2,458,195 + +2.6 2,458,195 + -1.0 2,458,195 + -0.1 2,458,195 + +0.0 2,458,195 + -0.4 2,458,195 + -0.4 2,458,195 + -1.7 2,458,195 + +0.3 2,458,195 + +1.0 2,458,195 + +0.0 2,458,195 + +1.3 2,458,195 + -0.2 2,458,195 + +0.4 2,458,195 + +1.0 2,458,195 + -1.2 2,458,195 + +0.6 2,458,195 + -1.4 2,458,195 + +0.0 2,458,195 + -1.9 2,458,195 + -0.1 2,458,195 + +0.0 2,458,195 + +0.6 2,458,195 + +0.6 2,458,195 + -0.8 2,458,195 + -0.6 2,458,195 + -1.6 2,458,195 + -0.2 2,458,195 + +0.4 2,458,195 + -1.0 2,458,195 + +1.6 2,458,195 + +2.2 2,458,196 - +0.0 2,458,196 - -0.2 2,458,196 - +0.2 2,458,196 - +1.6 2,458,196 - -0.4 2,458,196 - +0.3 2,458,196 - -0.4 2,458,196 - +0.4 2,458,196 - -0.3 2,458,196 - +1.0 2,458,196 - +1.6 2,458,196 - -0.4 2,458,196 - -0.4 2,458,196 - +0.0 2,458,196 - -0.2 2,458,196 - -1.6 2,458,196 - -2.6 2,458,196 - +0.0 2,458,196 - +1.6 2,458,196 - +0.6 2,458,196 - -0.9 2,458,196 - -0.2 2,458,196 - -1.5 2,458,196 - -1.5 2,458,196 - -0.1 2,458,196 - +1.6 2,458,196 - -0.6 2,458,196 - +0.6 2,458,196 - -1.4 2,458,199 + -0.7 2,458,199 + -2.3 2,458,199 + +0.6 2,458,199 + -0.7 2,458,199 + +0.0 2,458,199 + -0.4 2,458,200 - +0.0 2,458,200 - +0.0 2,458,200 - +0.7 2,458,200 - -0.5 2,458,200 - -0.7 2,458,228 + +0.3 2,458,229 - -0.4 2,458,274 + +0.2 2,458,274 + +0.6 2,458,275 - +1.6 2,458,280 + +0.0 2,458,281 - -1.0 2,458,281 - -1.7 2,458,281 - +1.0 2,458,281 - -0.4 2,458,281 - -0.5
Or see this region's nucleotide sequence