Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1536 and Pf1N1B4_1537 are separated by 10 nucleotides Pf1N1B4_1537 and Pf1N1B4_1538 are separated by 150 nucleotides Pf1N1B4_1538 and Pf1N1B4_1539 are separated by 31 nucleotides
Pf1N1B4_1536 - DamX, an inner membrane protein involved in bile resistance, at 1,771,504 to 1,773,096
_1536
Pf1N1B4_1537 - 3-dehydroquinate synthase (EC 4.2.3.4), at 1,773,107 to 1,774,207
_1537
Pf1N1B4_1538 - Shikimate kinase I (EC 2.7.1.71), at 1,774,358 to 1,774,849
_1538
Pf1N1B4_1539 - Type IV pilus biogenesis protein PilQ, at 1,774,881 to 1,776,956
_1539
Position (kb)
1773
1774
1775 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1772.305 kb on + strand, within Pf1N1B4_1536 at 1772.305 kb on + strand, within Pf1N1B4_1536 at 1772.305 kb on + strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.306 kb on - strand, within Pf1N1B4_1536 at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.334 kb on + strand at 1772.334 kb on + strand at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.334 kb on + strand, within Pf1N1B4_1536 at 1772.507 kb on + strand, within Pf1N1B4_1536 at 1772.507 kb on + strand, within Pf1N1B4_1536 at 1772.507 kb on + strand, within Pf1N1B4_1536 at 1772.507 kb on + strand, within Pf1N1B4_1536 at 1772.531 kb on + strand, within Pf1N1B4_1536 at 1772.531 kb on + strand, within Pf1N1B4_1536 at 1772.531 kb on + strand, within Pf1N1B4_1536 at 1772.531 kb on + strand, within Pf1N1B4_1536 at 1772.714 kb on + strand, within Pf1N1B4_1536 at 1772.714 kb on + strand, within Pf1N1B4_1536 at 1772.714 kb on + strand, within Pf1N1B4_1536 at 1772.714 kb on + strand, within Pf1N1B4_1536 at 1772.714 kb on + strand, within Pf1N1B4_1536 at 1772.715 kb on - strand, within Pf1N1B4_1536 at 1773.049 kb on + strand at 1773.049 kb on + strand at 1773.049 kb on + strand at 1773.049 kb on + strand at 1773.049 kb on + strand at 1773.050 kb on - strand at 1773.050 kb on - strand at 1773.050 kb on - strand at 1773.050 kb on - strand at 1773.050 kb on - strand at 1773.090 kb on + strand at 1773.090 kb on + strand at 1773.090 kb on + strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.091 kb on - strand at 1773.105 kb on + strand at 1773.105 kb on + strand at 1773.105 kb on + strand at 1773.105 kb on + strand at 1773.105 kb on + strand at 1773.106 kb on - strand at 1773.106 kb on - strand at 1773.106 kb on - strand at 1773.106 kb on - strand at 1773.106 kb on - strand at 1773.106 kb on - strand at 1773.108 kb on + strand at 1773.115 kb on + strand at 1773.115 kb on + strand at 1773.115 kb on + strand at 1773.135 kb on + strand at 1773.158 kb on + strand at 1773.158 kb on + strand at 1773.833 kb on + strand at 1773.833 kb on + strand, within Pf1N1B4_1537 at 1774.252 kb on + strand at 1774.581 kb on + strand, within Pf1N1B4_1538 at 1774.655 kb on + strand, within Pf1N1B4_1538 at 1774.932 kb on + strand at 1774.933 kb on - strand at 1774.990 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.018 kb on + strand at 1775.019 kb on - strand at 1775.022 kb on + strand at 1775.022 kb on + strand at 1775.022 kb on + strand at 1775.022 kb on + strand at 1775.022 kb on + strand at 1775.022 kb on + strand at 1775.039 kb on + strand at 1775.039 kb on + strand at 1775.039 kb on + strand at 1775.039 kb on + strand at 1775.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,772,305 + Pf1N1B4_1536 0.50 +0.4 1,772,305 + Pf1N1B4_1536 0.50 -0.3 1,772,305 + Pf1N1B4_1536 0.50 +0.2 1,772,306 - Pf1N1B4_1536 0.50 +0.8 1,772,306 - Pf1N1B4_1536 0.50 -1.2 1,772,306 - Pf1N1B4_1536 0.50 -0.2 1,772,306 - Pf1N1B4_1536 0.50 +0.2 1,772,306 - Pf1N1B4_1536 0.50 +0.1 1,772,306 - Pf1N1B4_1536 0.50 -0.4 1,772,306 - Pf1N1B4_1536 0.50 -0.0 1,772,306 - Pf1N1B4_1536 0.50 -1.1 1,772,306 - Pf1N1B4_1536 0.50 +0.2 1,772,306 - Pf1N1B4_1536 0.50 +0.4 1,772,306 - Pf1N1B4_1536 0.50 -0.3 1,772,334 + Pf1N1B4_1536 0.52 -0.5 1,772,334 + Pf1N1B4_1536 0.52 +1.4 1,772,334 + +1.6 1,772,334 + -1.0 1,772,334 + Pf1N1B4_1536 0.52 -2.1 1,772,334 + Pf1N1B4_1536 0.52 -0.7 1,772,334 + Pf1N1B4_1536 0.52 -1.2 1,772,334 + Pf1N1B4_1536 0.52 +0.4 1,772,507 + Pf1N1B4_1536 0.63 -0.4 1,772,507 + Pf1N1B4_1536 0.63 +0.6 1,772,507 + Pf1N1B4_1536 0.63 +0.4 1,772,507 + Pf1N1B4_1536 0.63 -2.7 1,772,531 + Pf1N1B4_1536 0.64 +1.1 1,772,531 + Pf1N1B4_1536 0.64 -1.5 1,772,531 + Pf1N1B4_1536 0.64 -1.4 1,772,531 + Pf1N1B4_1536 0.64 -0.4 1,772,714 + Pf1N1B4_1536 0.76 -0.4 1,772,714 + Pf1N1B4_1536 0.76 +0.1 1,772,714 + Pf1N1B4_1536 0.76 +1.2 1,772,714 + Pf1N1B4_1536 0.76 -0.4 1,772,714 + Pf1N1B4_1536 0.76 -1.9 1,772,715 - Pf1N1B4_1536 0.76 -0.8 1,773,049 + -1.0 1,773,049 + +0.0 1,773,049 + +0.1 1,773,049 + +1.6 1,773,049 + +1.6 1,773,050 - -2.2 1,773,050 - +0.1 1,773,050 - -0.8 1,773,050 - -0.6 1,773,050 - +0.6 1,773,090 + +3.0 1,773,090 + +0.2 1,773,090 + -0.0 1,773,091 - -0.9 1,773,091 - -1.4 1,773,091 - +0.1 1,773,091 - -0.2 1,773,091 - +1.6 1,773,091 - -0.3 1,773,091 - -0.0 1,773,105 + -1.6 1,773,105 + +0.7 1,773,105 + -1.4 1,773,105 + -0.4 1,773,105 + +0.0 1,773,106 - +1.1 1,773,106 - -0.4 1,773,106 - +0.9 1,773,106 - -0.3 1,773,106 - +0.7 1,773,106 - +1.9 1,773,108 + +0.3 1,773,115 + -1.6 1,773,115 + -0.6 1,773,115 + +0.4 1,773,135 + -0.0 1,773,158 + -0.4 1,773,158 + -0.4 1,773,833 + +0.6 1,773,833 + Pf1N1B4_1537 0.66 +0.6 1,774,252 + +1.6 1,774,581 + Pf1N1B4_1538 0.45 -0.0 1,774,655 + Pf1N1B4_1538 0.60 -0.8 1,774,932 + -1.0 1,774,933 - -0.3 1,774,990 + -0.6 1,775,018 + -0.7 1,775,018 + -1.0 1,775,018 + -0.6 1,775,018 + -0.4 1,775,018 + -0.4 1,775,018 + -0.0 1,775,018 + +1.6 1,775,018 + -2.2 1,775,018 + -0.9 1,775,018 + -0.8 1,775,018 + +0.8 1,775,018 + +0.6 1,775,019 - +1.0 1,775,022 + -0.8 1,775,022 + +0.3 1,775,022 + -1.6 1,775,022 + -0.2 1,775,022 + -1.0 1,775,022 + +0.1 1,775,039 + +0.6 1,775,039 + +2.0 1,775,039 + -0.8 1,775,039 + +1.8 1,775,040 - -0.8
Or see this region's nucleotide sequence