Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1019 and Pf1N1B4_1020 overlap by 8 nucleotides Pf1N1B4_1020 and Pf1N1B4_1021 are separated by 204 nucleotides
Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414
_1019
Pf1N1B4_1020 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 1,155,407 to 1,156,405
_1020
Pf1N1B4_1021 - Leucine dehydrogenase (EC 1.4.1.9), at 1,156,610 to 1,157,629
_1021
Position (kb)
1155
1156
1157 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1154.432 kb on + strand at 1154.433 kb on - strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.504 kb on + strand at 1154.577 kb on - strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.880 kb on - strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.311 kb on - strand, within Pf1N1B4_1019 at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.588 kb on - strand, within Pf1N1B4_1020 at 1155.631 kb on + strand, within Pf1N1B4_1020 at 1156.157 kb on - strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.239 kb on + strand, within Pf1N1B4_1020 at 1156.240 kb on - strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.251 kb on - strand, within Pf1N1B4_1020 at 1156.251 kb on - strand at 1156.251 kb on - strand, within Pf1N1B4_1020 at 1156.475 kb on + strand at 1156.475 kb on + strand at 1156.618 kb on - strand at 1156.618 kb on - strand at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.797 kb on + strand, within Pf1N1B4_1021 at 1156.797 kb on + strand, within Pf1N1B4_1021 at 1156.809 kb on + strand, within Pf1N1B4_1021 at 1156.810 kb on - strand, within Pf1N1B4_1021 at 1156.918 kb on + strand, within Pf1N1B4_1021 at 1156.918 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.950 kb on - strand, within Pf1N1B4_1021 at 1156.950 kb on - strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.141 kb on - strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.352 kb on - strand, within Pf1N1B4_1021 at 1157.352 kb on - strand, within Pf1N1B4_1021
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,154,432 + -0.3 1,154,433 - -0.7 1,154,486 + -1.0 1,154,486 + +1.6 1,154,486 + +0.6 1,154,486 + +1.0 1,154,486 + -0.6 1,154,486 + -1.2 1,154,486 + +1.6 1,154,486 + -0.6 1,154,486 + -0.2 1,154,486 + +0.7 1,154,486 + +0.3 1,154,486 + -0.9 1,154,487 - -0.2 1,154,487 - +0.3 1,154,487 - -0.3 1,154,487 - -0.3 1,154,487 - +0.6 1,154,504 + -0.3 1,154,577 - Pf1N1B4_1019 0.15 +0.8 1,154,793 + Pf1N1B4_1019 0.37 -0.4 1,154,793 + Pf1N1B4_1019 0.37 +0.8 1,154,793 + Pf1N1B4_1019 0.37 -0.1 1,154,794 - Pf1N1B4_1019 0.37 +1.2 1,154,794 - Pf1N1B4_1019 0.37 -0.5 1,154,794 - Pf1N1B4_1019 0.37 +0.3 1,154,880 - Pf1N1B4_1019 0.46 +1.4 1,154,902 + Pf1N1B4_1019 0.48 +0.3 1,154,902 + Pf1N1B4_1019 0.48 +0.6 1,154,903 - Pf1N1B4_1019 0.48 -1.4 1,154,903 - Pf1N1B4_1019 0.48 -1.9 1,155,146 + Pf1N1B4_1019 0.73 -0.7 1,155,146 + Pf1N1B4_1019 0.73 -0.7 1,155,146 + Pf1N1B4_1019 0.73 +1.4 1,155,146 + Pf1N1B4_1019 0.73 -0.2 1,155,147 - Pf1N1B4_1019 0.73 -0.6 1,155,147 - Pf1N1B4_1019 0.73 -0.2 1,155,147 - +3.2 1,155,147 - Pf1N1B4_1019 0.73 -0.3 1,155,311 - Pf1N1B4_1019 0.89 +0.5 1,155,495 + -1.7 1,155,495 + +1.3 1,155,495 + -1.2 1,155,588 - Pf1N1B4_1020 0.18 -0.2 1,155,631 + Pf1N1B4_1020 0.22 -0.7 1,156,157 - Pf1N1B4_1020 0.75 +1.6 1,156,179 + Pf1N1B4_1020 0.77 -0.6 1,156,179 + Pf1N1B4_1020 0.77 +0.8 1,156,180 - Pf1N1B4_1020 0.77 +0.6 1,156,180 - Pf1N1B4_1020 0.77 -1.7 1,156,180 - Pf1N1B4_1020 0.77 -0.3 1,156,239 + Pf1N1B4_1020 0.83 -0.7 1,156,240 - Pf1N1B4_1020 0.83 -0.7 1,156,250 + Pf1N1B4_1020 0.84 -0.0 1,156,250 + Pf1N1B4_1020 0.84 -1.5 1,156,250 + Pf1N1B4_1020 0.84 -0.7 1,156,250 + Pf1N1B4_1020 0.84 +0.1 1,156,251 - Pf1N1B4_1020 0.84 +0.3 1,156,251 - -0.7 1,156,251 - Pf1N1B4_1020 0.84 +1.4 1,156,475 + -0.2 1,156,475 + -0.6 1,156,618 - -1.1 1,156,618 - +0.8 1,156,770 + Pf1N1B4_1021 0.16 -1.8 1,156,770 + Pf1N1B4_1021 0.16 -2.4 1,156,770 + Pf1N1B4_1021 0.16 -0.6 1,156,770 + Pf1N1B4_1021 0.16 +0.6 1,156,770 + Pf1N1B4_1021 0.16 -0.6 1,156,770 + Pf1N1B4_1021 0.16 -1.4 1,156,770 + Pf1N1B4_1021 0.16 -0.9 1,156,770 + Pf1N1B4_1021 0.16 +0.4 1,156,770 + Pf1N1B4_1021 0.16 -2.0 1,156,771 - Pf1N1B4_1021 0.16 -0.9 1,156,771 - Pf1N1B4_1021 0.16 +0.3 1,156,771 - Pf1N1B4_1021 0.16 -0.2 1,156,771 - Pf1N1B4_1021 0.16 -0.2 1,156,771 - Pf1N1B4_1021 0.16 +2.3 1,156,771 - Pf1N1B4_1021 0.16 +0.9 1,156,771 - Pf1N1B4_1021 0.16 +0.0 1,156,771 - Pf1N1B4_1021 0.16 -2.4 1,156,771 - Pf1N1B4_1021 0.16 +1.9 1,156,797 + Pf1N1B4_1021 0.18 -1.9 1,156,797 + Pf1N1B4_1021 0.18 -0.6 1,156,809 + Pf1N1B4_1021 0.20 +2.6 1,156,810 - Pf1N1B4_1021 0.20 +1.2 1,156,918 + Pf1N1B4_1021 0.30 +0.7 1,156,918 + Pf1N1B4_1021 0.30 +0.0 1,156,949 + Pf1N1B4_1021 0.33 +0.1 1,156,949 + Pf1N1B4_1021 0.33 -3.1 1,156,949 + Pf1N1B4_1021 0.33 +0.2 1,156,949 + Pf1N1B4_1021 0.33 -0.4 1,156,949 + Pf1N1B4_1021 0.33 +0.6 1,156,950 - Pf1N1B4_1021 0.33 -0.2 1,156,950 - Pf1N1B4_1021 0.33 -0.4 1,157,072 + Pf1N1B4_1021 0.45 -0.9 1,157,072 + Pf1N1B4_1021 0.45 +0.2 1,157,072 + Pf1N1B4_1021 0.45 -0.4 1,157,072 + Pf1N1B4_1021 0.45 +0.2 1,157,141 - Pf1N1B4_1021 0.52 -0.7 1,157,307 + Pf1N1B4_1021 0.68 +0.7 1,157,307 + Pf1N1B4_1021 0.68 +0.4 1,157,307 + Pf1N1B4_1021 0.68 +1.2 1,157,308 - Pf1N1B4_1021 0.68 +2.8 1,157,308 - Pf1N1B4_1021 0.68 -1.7 1,157,308 - Pf1N1B4_1021 0.68 +0.0 1,157,352 - Pf1N1B4_1021 0.73 +0.7 1,157,352 - Pf1N1B4_1021 0.73 -1.6
Or see this region's nucleotide sequence