Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1381 and tRNA-Asp are separated by 446 nucleotides tRNA-Asp and tRNA-Asp are separated by 53 nucleotides tRNA-Asp and BT1382 are separated by 253 nucleotides
BT1381: BT1381 - formyltetrahydrofolate deformylase (NCBI ptt file), at 1,708,512 to 1,709,366
BT1381
BT_t25: tRNA-Asp - tRNA-Asp (RefSeq), at 1,709,813 to 1,709,886
tRNA-Asp
BT_t26: tRNA-Asp - tRNA-Asp (RefSeq), at 1,709,940 to 1,710,013
tRNA-Asp
BT1382: BT1382 - conserved hypothetical protein (NCBI ptt file), at 1,710,267 to 1,710,860
BT1382
Position (kb)
1709
1710 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1708.851 kb on - strand, within BT1381 at 1708.851 kb on - strand, within BT1381 at 1708.952 kb on - strand, within BT1381 at 1709.006 kb on + strand, within BT1381 at 1709.009 kb on + strand, within BT1381 at 1709.016 kb on + strand, within BT1381 at 1709.156 kb on - strand, within BT1381 at 1709.170 kb on + strand, within BT1381 at 1709.221 kb on - strand, within BT1381 at 1709.221 kb on - strand, within BT1381 at 1709.223 kb on - strand, within BT1381 at 1709.257 kb on + strand, within BT1381 at 1709.270 kb on - strand, within BT1381 at 1709.276 kb on + strand, within BT1381 at 1709.360 kb on + strand at 1709.513 kb on + strand at 1709.588 kb on - strand at 1709.693 kb on + strand at 1709.898 kb on + strand at 1710.110 kb on - strand at 1710.133 kb on + strand at 1710.168 kb on + strand at 1710.178 kb on + strand at 1710.179 kb on - strand at 1710.182 kb on + strand at 1710.194 kb on + strand at 1710.247 kb on + strand at 1710.292 kb on + strand at 1710.323 kb on + strand at 1710.326 kb on - strand at 1710.351 kb on - strand, within BT1382 at 1710.373 kb on - strand, within BT1382 at 1710.433 kb on + strand, within BT1382 at 1710.487 kb on + strand, within BT1382 at 1710.530 kb on - strand, within BT1382 at 1710.561 kb on - strand, within BT1382 at 1710.605 kb on + strand, within BT1382 at 1710.605 kb on + strand, within BT1382 at 1710.628 kb on + strand, within BT1382 at 1710.644 kb on + strand, within BT1382 at 1710.654 kb on + strand, within BT1382 at 1710.681 kb on + strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.699 kb on + strand, within BT1382 at 1710.718 kb on + strand, within BT1382 at 1710.778 kb on + strand, within BT1382 at 1710.779 kb on - strand, within BT1382 at 1710.781 kb on + strand, within BT1382 at 1710.783 kb on + strand, within BT1382 at 1710.784 kb on - strand, within BT1382 at 1710.791 kb on - strand, within BT1382 at 1710.800 kb on + strand, within BT1382
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 1,708,851 - BT1381 0.40 -1.9 1,708,851 - BT1381 0.40 -0.9 1,708,952 - BT1381 0.51 -0.9 1,709,006 + BT1381 0.58 -1.9 1,709,009 + BT1381 0.58 -3.1 1,709,016 + BT1381 0.59 -1.5 1,709,156 - BT1381 0.75 +0.1 1,709,170 + BT1381 0.77 -1.9 1,709,221 - BT1381 0.83 -4.8 1,709,221 - BT1381 0.83 -3.2 1,709,223 - BT1381 0.83 -1.7 1,709,257 + BT1381 0.87 -1.9 1,709,270 - BT1381 0.89 -1.5 1,709,276 + BT1381 0.89 -1.5 1,709,360 + -0.9 1,709,513 + -0.9 1,709,588 - -1.6 1,709,693 + +0.1 1,709,898 + -1.2 1,710,110 - +0.7 1,710,133 + +0.1 1,710,168 + -2.2 1,710,178 + -1.5 1,710,179 - -1.5 1,710,182 + -0.5 1,710,194 + -1.5 1,710,247 + +0.1 1,710,292 + -3.1 1,710,323 + -4.7 1,710,326 - -2.1 1,710,351 - BT1382 0.14 -2.2 1,710,373 - BT1382 0.18 -0.9 1,710,433 + BT1382 0.28 +1.7 1,710,487 + BT1382 0.37 -1.7 1,710,530 - BT1382 0.44 -0.9 1,710,561 - BT1382 0.49 -3.0 1,710,605 + BT1382 0.57 -0.9 1,710,605 + BT1382 0.57 -4.2 1,710,628 + BT1382 0.61 +0.1 1,710,644 + BT1382 0.63 +0.4 1,710,654 + BT1382 0.65 +0.1 1,710,681 + BT1382 0.70 +0.1 1,710,684 - BT1382 0.70 +2.4 1,710,684 - BT1382 0.70 -0.5 1,710,684 - BT1382 0.70 +0.1 1,710,699 + BT1382 0.73 -1.4 1,710,718 + BT1382 0.76 -1.5 1,710,778 + BT1382 0.86 -0.6 1,710,779 - BT1382 0.86 -1.7 1,710,781 + BT1382 0.87 +0.1 1,710,783 + BT1382 0.87 -0.9 1,710,784 - BT1382 0.87 -0.9 1,710,791 - BT1382 0.88 -2.0 1,710,800 + BT1382 0.90 -1.9
Or see this region's nucleotide sequence