Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3974 and BT3975 are separated by 123 nucleotides BT3975 and BT3976 are separated by 80 nucleotides BT3976 and BT3977 are separated by 80 nucleotides
BT3974: BT3974 - conserved hypothetical protein (NCBI ptt file), at 5,174,933 to 5,175,727
BT3974
BT3975: BT3975 - 3-dehydroquinate synthase (NCBI ptt file), at 5,175,851 to 5,176,912
BT3975
BT3976: BT3976 - hypothetical protein (NCBI ptt file), at 5,176,993 to 5,177,397
BT3976
BT3977: BT3977 - conserved hypothetical protein (NCBI ptt file), at 5,177,478 to 5,180,291
BT3977
Position (kb) 5175
5176
5177 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5174.870 kb on - strand at 5174.873 kb on - strand at 5174.897 kb on + strand at 5174.966 kb on - strand at 5174.968 kb on + strand at 5175.017 kb on - strand, within BT3974 at 5175.021 kb on + strand, within BT3974 at 5175.121 kb on + strand, within BT3974 at 5175.122 kb on - strand, within BT3974 at 5175.200 kb on - strand, within BT3974 at 5175.277 kb on + strand, within BT3974 at 5175.296 kb on - strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.298 kb on - strand, within BT3974 at 5175.299 kb on + strand, within BT3974 at 5175.300 kb on - strand, within BT3974 at 5175.333 kb on - strand, within BT3974 at 5175.336 kb on + strand, within BT3974 at 5175.404 kb on - strand, within BT3974 at 5175.438 kb on + strand, within BT3974 at 5175.461 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.562 kb on - strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.613 kb on + strand, within BT3974 at 5175.614 kb on - strand, within BT3974 at 5175.715 kb on + strand at 5175.716 kb on - strand at 5175.731 kb on - strand at 5175.831 kb on + strand at 5175.831 kb on + strand at 5175.863 kb on - strand at 5176.992 kb on + strand at 5177.032 kb on + strand at 5177.045 kb on + strand, within BT3976 at 5177.113 kb on + strand, within BT3976 at 5177.428 kb on + strand at 5177.429 kb on - strand at 5177.450 kb on - strand at 5177.452 kb on + strand at 5177.460 kb on + strand at 5177.470 kb on + strand at 5177.471 kb on - strand at 5177.507 kb on + strand at 5177.508 kb on - strand at 5177.543 kb on + strand at 5177.544 kb on - strand at 5177.661 kb on - strand at 5177.664 kb on - strand at 5177.707 kb on - strand at 5177.724 kb on + strand at 5177.724 kb on + strand at 5177.724 kb on + strand at 5177.880 kb on - strand, within BT3977 at 5177.880 kb on - strand, within BT3977
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 5,174,870 - -3.0 5,174,873 - +0.2 5,174,897 + -0.5 5,174,966 - -1.3 5,174,968 + -2.0 5,175,017 - BT3974 0.11 -2.6 5,175,021 + BT3974 0.11 +0.8 5,175,121 + BT3974 0.24 -1.0 5,175,122 - BT3974 0.24 -1.0 5,175,200 - BT3974 0.34 -1.0 5,175,277 + BT3974 0.43 -1.9 5,175,296 - BT3974 0.46 -0.0 5,175,297 + BT3974 0.46 +1.3 5,175,297 + BT3974 0.46 -0.2 5,175,297 + BT3974 0.46 -0.5 5,175,297 + BT3974 0.46 -0.0 5,175,298 - BT3974 0.46 -0.0 5,175,299 + BT3974 0.46 +1.0 5,175,300 - BT3974 0.46 -2.0 5,175,333 - BT3974 0.50 -0.7 5,175,336 + BT3974 0.51 -1.9 5,175,404 - BT3974 0.59 -1.0 5,175,438 + BT3974 0.64 -2.6 5,175,461 - BT3974 0.66 +1.0 5,175,485 - BT3974 0.69 -0.5 5,175,485 - BT3974 0.69 -1.5 5,175,562 - BT3974 0.79 -2.0 5,175,573 + BT3974 0.81 +1.1 5,175,573 + BT3974 0.81 -1.0 5,175,613 + BT3974 0.86 -2.9 5,175,614 - BT3974 0.86 -1.2 5,175,715 + -2.0 5,175,716 - -1.6 5,175,731 - -1.0 5,175,831 + -3.7 5,175,831 + +0.2 5,175,863 - -1.4 5,176,992 + -1.0 5,177,032 + -2.3 5,177,045 + BT3976 0.13 -1.3 5,177,113 + BT3976 0.30 -0.8 5,177,428 + -0.0 5,177,429 - +0.3 5,177,450 - -2.0 5,177,452 + -0.6 5,177,460 + -0.4 5,177,470 + -2.5 5,177,471 - -2.6 5,177,507 + -1.0 5,177,508 - +0.5 5,177,543 + -1.0 5,177,544 - -3.0 5,177,661 - -1.3 5,177,664 - -1.6 5,177,707 - +1.5 5,177,724 + -2.0 5,177,724 + -0.0 5,177,724 + -2.4 5,177,880 - BT3977 0.14 -1.6 5,177,880 - BT3977 0.14 -0.0
Or see this region's nucleotide sequence