Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3863 and BT3864 are separated by 81 nucleotides BT3864 and BT3865 are separated by 49 nucleotides BT3865 and BT3866 are separated by 168 nucleotides
BT3863: BT3863 - conserved hypothetical protein (NCBI ptt file), at 5,031,632 to 5,034,007
BT3863
BT3864: BT3864 - tryptophanyl-tRNA synthetase (NCBI ptt file), at 5,034,089 to 5,035,189
BT3864
BT3865: BT3865 - hypothetical protein (NCBI ptt file), at 5,035,239 to 5,035,451
BT3865
BT3866: BT3866 - ammonium-dependent carbamoyl phosphate synthetase CarB2 (EC 6.3.4.16) (from data) , at 5,035,620 to 5,038,841
BT3866
Position (kb)
5034
5035
5036 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5033.092 kb on - strand, within BT3863 at 5033.128 kb on + strand, within BT3863 at 5033.131 kb on - strand, within BT3863 at 5033.131 kb on - strand, within BT3863 at 5033.133 kb on - strand, within BT3863 at 5033.139 kb on + strand, within BT3863 at 5033.282 kb on + strand, within BT3863 at 5033.283 kb on - strand, within BT3863 at 5033.283 kb on - strand, within BT3863 at 5033.331 kb on - strand, within BT3863 at 5033.335 kb on + strand, within BT3863 at 5033.341 kb on + strand, within BT3863 at 5033.374 kb on + strand, within BT3863 at 5033.375 kb on - strand, within BT3863 at 5033.375 kb on - strand, within BT3863 at 5033.430 kb on - strand, within BT3863 at 5033.474 kb on + strand, within BT3863 at 5033.507 kb on + strand, within BT3863 at 5033.507 kb on + strand, within BT3863 at 5033.507 kb on + strand, within BT3863 at 5033.518 kb on + strand, within BT3863 at 5033.518 kb on + strand, within BT3863 at 5033.519 kb on - strand, within BT3863 at 5033.519 kb on - strand, within BT3863 at 5033.520 kb on + strand, within BT3863 at 5033.520 kb on + strand, within BT3863 at 5033.541 kb on - strand, within BT3863 at 5033.596 kb on + strand, within BT3863 at 5033.596 kb on + strand, within BT3863 at 5033.597 kb on - strand, within BT3863 at 5033.604 kb on - strand, within BT3863 at 5033.606 kb on + strand, within BT3863 at 5033.607 kb on - strand, within BT3863 at 5033.607 kb on - strand, within BT3863 at 5033.777 kb on - strand at 5033.878 kb on - strand at 5033.910 kb on + strand at 5033.911 kb on - strand at 5033.911 kb on - strand at 5033.965 kb on - strand at 5034.015 kb on - strand at 5034.016 kb on + strand at 5034.017 kb on - strand at 5034.017 kb on - strand at 5034.019 kb on - strand at 5034.062 kb on - strand at 5034.066 kb on + strand at 5034.066 kb on + strand at 5034.069 kb on + strand at 5034.070 kb on - strand at 5034.082 kb on - strand at 5034.084 kb on + strand at 5034.085 kb on - strand at 5035.263 kb on + strand, within BT3865 at 5035.264 kb on - strand, within BT3865 at 5035.274 kb on + strand, within BT3865 at 5035.274 kb on + strand, within BT3865 at 5035.281 kb on + strand, within BT3865 at 5035.281 kb on + strand, within BT3865 at 5035.282 kb on - strand, within BT3865 at 5035.392 kb on - strand, within BT3865 at 5035.524 kb on + strand at 5035.525 kb on - strand at 5035.736 kb on + strand at 5035.736 kb on + strand at 5035.799 kb on + strand at 5035.940 kb on + strand at 5035.945 kb on - strand, within BT3866 at 5035.958 kb on - strand, within BT3866 at 5035.980 kb on - strand, within BT3866 at 5035.986 kb on + strand, within BT3866 at 5035.987 kb on - strand, within BT3866 at 5035.987 kb on - strand, within BT3866 at 5036.116 kb on + strand, within BT3866 at 5036.116 kb on + strand, within BT3866
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 5,033,092 - BT3863 0.61 +0.0 5,033,128 + BT3863 0.63 +1.0 5,033,131 - BT3863 0.63 -1.6 5,033,131 - BT3863 0.63 +0.0 5,033,133 - BT3863 0.63 -2.0 5,033,139 + BT3863 0.63 -0.6 5,033,282 + BT3863 0.69 -0.4 5,033,283 - BT3863 0.69 -3.5 5,033,283 - BT3863 0.69 -1.0 5,033,331 - BT3863 0.72 -2.1 5,033,335 + BT3863 0.72 +0.6 5,033,341 + BT3863 0.72 -1.1 5,033,374 + BT3863 0.73 -0.5 5,033,375 - BT3863 0.73 -1.0 5,033,375 - BT3863 0.73 -2.0 5,033,430 - BT3863 0.76 -0.3 5,033,474 + BT3863 0.78 -1.6 5,033,507 + BT3863 0.79 -0.6 5,033,507 + BT3863 0.79 +0.0 5,033,507 + BT3863 0.79 +0.0 5,033,518 + BT3863 0.79 -1.6 5,033,518 + BT3863 0.79 -1.1 5,033,519 - BT3863 0.79 -0.6 5,033,519 - BT3863 0.79 -2.3 5,033,520 + BT3863 0.79 -1.0 5,033,520 + BT3863 0.79 -1.9 5,033,541 - BT3863 0.80 -0.4 5,033,596 + BT3863 0.83 -2.0 5,033,596 + BT3863 0.83 -0.4 5,033,597 - BT3863 0.83 +0.0 5,033,604 - BT3863 0.83 -0.7 5,033,606 + BT3863 0.83 +1.6 5,033,607 - BT3863 0.83 -1.3 5,033,607 - BT3863 0.83 -0.8 5,033,777 - -2.0 5,033,878 - -0.4 5,033,910 + +1.0 5,033,911 - -1.2 5,033,911 - +1.6 5,033,965 - +0.3 5,034,015 - -1.0 5,034,016 + -1.7 5,034,017 - -1.0 5,034,017 - +2.8 5,034,019 - +0.0 5,034,062 - +0.0 5,034,066 + -1.0 5,034,066 + -1.6 5,034,069 + -0.6 5,034,070 - +0.0 5,034,082 - -1.0 5,034,084 + -0.4 5,034,085 - -1.8 5,035,263 + BT3865 0.11 -1.0 5,035,264 - BT3865 0.12 +0.0 5,035,274 + BT3865 0.16 -3.0 5,035,274 + BT3865 0.16 -0.4 5,035,281 + BT3865 0.20 +1.0 5,035,281 + BT3865 0.20 -0.8 5,035,282 - BT3865 0.20 -1.0 5,035,392 - BT3865 0.72 -0.7 5,035,524 + -1.3 5,035,525 - +0.4 5,035,736 + +0.0 5,035,736 + -1.6 5,035,799 + +1.0 5,035,940 + -1.0 5,035,945 - BT3866 0.10 +0.0 5,035,958 - BT3866 0.10 +1.2 5,035,980 - BT3866 0.11 +0.0 5,035,986 + BT3866 0.11 +0.0 5,035,987 - BT3866 0.11 -1.2 5,035,987 - BT3866 0.11 +0.0 5,036,116 + BT3866 0.15 -0.1 5,036,116 + BT3866 0.15 -1.6
Or see this region's nucleotide sequence