Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3216 and BT3217 are separated by 30 nucleotides BT3217 and BT3218 overlap by 4 nucleotides BT3218 and BT3219 are separated by 207 nucleotides
BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471
BT3216
BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693
BT3217
BT3218: BT3218 - hypothetical protein (NCBI ptt file), at 4,108,690 to 4,108,923
BT3218
BT3219: BT3219 - S-adenosylmethionine synthetase (NCBI ptt file), at 4,109,131 to 4,110,423
BT3219
Position (kb)
4109
4110
4111 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4108.563 kb on - strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.572 kb on - strand, within BT3217 at 4108.583 kb on - strand, within BT3217 at 4108.613 kb on + strand, within BT3217 at 4108.614 kb on - strand, within BT3217 at 4108.624 kb on - strand, within BT3217 at 4108.643 kb on + strand, within BT3217 at 4108.648 kb on - strand, within BT3217 at 4108.687 kb on + strand at 4108.694 kb on - strand at 4108.708 kb on - strand at 4108.729 kb on + strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.787 kb on + strand, within BT3218 at 4108.788 kb on - strand, within BT3218 at 4108.868 kb on + strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.908 kb on + strand at 4108.909 kb on - strand at 4108.911 kb on - strand at 4108.911 kb on - strand at 4108.917 kb on + strand at 4108.939 kb on - strand at 4108.958 kb on + strand at 4109.110 kb on + strand at 4109.110 kb on + strand at 4110.444 kb on + strand at 4110.445 kb on - strand at 4110.467 kb on + strand at 4110.537 kb on + strand at 4110.545 kb on - strand at 4110.551 kb on + strand at 4110.577 kb on + strand at 4110.577 kb on + strand at 4110.584 kb on + strand at 4110.585 kb on - strand at 4110.593 kb on + strand at 4110.605 kb on - strand at 4110.629 kb on - strand at 4110.680 kb on - strand at 4110.682 kb on - strand at 4110.687 kb on - strand at 4110.687 kb on - strand at 4110.731 kb on - strand at 4110.739 kb on + strand at 4110.739 kb on + strand at 4110.752 kb on + strand at 4110.775 kb on - strand at 4110.805 kb on + strand at 4110.810 kb on + strand at 4110.810 kb on + strand at 4110.811 kb on - strand at 4110.815 kb on + strand at 4110.816 kb on - strand at 4110.819 kb on - strand at 4110.819 kb on - strand at 4110.903 kb on + strand at 4110.967 kb on + strand at 4110.968 kb on - strand at 4111.017 kb on + strand at 4111.146 kb on + strand at 4111.147 kb on - strand at 4111.165 kb on - strand at 4111.207 kb on - strand at 4111.208 kb on + strand at 4111.208 kb on + strand at 4111.209 kb on - strand at 4111.227 kb on + strand at 4111.258 kb on - strand at 4111.263 kb on - strand at 4111.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 4,108,563 - BT3217 0.32 -1.1 4,108,571 + BT3217 0.36 -1.6 4,108,571 + BT3217 0.36 -1.0 4,108,572 - BT3217 0.36 -3.3 4,108,583 - BT3217 0.42 -1.2 4,108,613 + BT3217 0.58 -1.2 4,108,614 - BT3217 0.58 +0.2 4,108,624 - BT3217 0.64 -1.0 4,108,643 + BT3217 0.73 -0.2 4,108,648 - BT3217 0.76 -1.2 4,108,687 + -1.4 4,108,694 - -2.2 4,108,708 - -1.7 4,108,729 + BT3218 0.17 +0.6 4,108,756 - BT3218 0.28 -0.6 4,108,756 - BT3218 0.28 -0.5 4,108,756 - BT3218 0.28 -1.5 4,108,787 + BT3218 0.41 -0.5 4,108,788 - BT3218 0.42 -0.2 4,108,868 + BT3218 0.76 -1.8 4,108,877 - BT3218 0.80 -0.2 4,108,877 - BT3218 0.80 -3.4 4,108,908 + -1.6 4,108,909 - -1.8 4,108,911 - -1.6 4,108,911 - -1.8 4,108,917 + -1.2 4,108,939 - -0.2 4,108,958 + -2.2 4,109,110 + -2.8 4,109,110 + -1.2 4,110,444 + -0.8 4,110,445 - -2.6 4,110,467 + -3.5 4,110,537 + +1.4 4,110,545 - -2.0 4,110,551 + -1.8 4,110,577 + -2.2 4,110,577 + -2.2 4,110,584 + -2.8 4,110,585 - +0.1 4,110,593 + -1.7 4,110,605 - -1.4 4,110,629 - -1.6 4,110,680 - -1.6 4,110,682 - -0.4 4,110,687 - -1.2 4,110,687 - +0.4 4,110,731 - -1.8 4,110,739 + -2.0 4,110,739 + -3.2 4,110,752 + -1.2 4,110,775 - -1.0 4,110,805 + -0.7 4,110,810 + -1.2 4,110,810 + -2.2 4,110,811 - -1.4 4,110,815 + +0.8 4,110,816 - -1.5 4,110,819 - -0.5 4,110,819 - +0.5 4,110,903 + -0.6 4,110,967 + -2.3 4,110,968 - -3.1 4,111,017 + -1.8 4,111,146 + -3.2 4,111,147 - -1.5 4,111,165 - +0.3 4,111,207 - -0.8 4,111,208 + -2.7 4,111,208 + -1.3 4,111,209 - -1.9 4,111,227 + -2.5 4,111,258 - -2.0 4,111,263 - -1.7 4,111,335 + -2.2
Or see this region's nucleotide sequence