Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2747 and BT2748 are separated by 16 nucleotides BT2748 and BT2749 are separated by 94 nucleotides
BT2747: BT2747 - 3-deoxy-D-manno-octulosonic-acid transferase (NCBI ptt file), at 3,403,559 to 3,404,782
BT2747
BT2748: BT2748 - glutamyl-tRNA synthetase (NCBI ptt file), at 3,404,799 to 3,406,313
BT2748
BT2749: BT2749 - putative membrane-associated HD superfamily hydrolase (NCBI ptt file), at 3,406,408 to 3,408,480
BT2749
Position (kb)
3404
3405
3406
3407 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3404.801 kb on - strand at 3406.324 kb on - strand at 3406.324 kb on - strand at 3406.333 kb on - strand at 3406.416 kb on + strand at 3406.416 kb on + strand at 3406.417 kb on - strand at 3406.450 kb on - strand at 3406.452 kb on - strand at 3406.452 kb on - strand at 3406.455 kb on - strand at 3406.542 kb on - strand at 3406.657 kb on + strand, within BT2749 at 3406.657 kb on + strand, within BT2749 at 3406.658 kb on - strand, within BT2749 at 3406.658 kb on - strand, within BT2749 at 3406.711 kb on + strand, within BT2749 at 3406.716 kb on + strand, within BT2749 at 3406.733 kb on + strand, within BT2749 at 3406.750 kb on + strand, within BT2749 at 3406.759 kb on + strand, within BT2749 at 3406.818 kb on - strand, within BT2749 at 3406.896 kb on + strand, within BT2749 at 3406.897 kb on - strand, within BT2749 at 3406.898 kb on + strand, within BT2749 at 3406.898 kb on + strand, within BT2749 at 3406.898 kb on + strand, within BT2749 at 3406.899 kb on - strand, within BT2749 at 3406.900 kb on + strand, within BT2749 at 3406.900 kb on + strand, within BT2749 at 3406.901 kb on - strand, within BT2749 at 3406.901 kb on - strand, within BT2749 at 3406.901 kb on - strand, within BT2749 at 3406.918 kb on + strand, within BT2749 at 3406.924 kb on + strand, within BT2749 at 3406.983 kb on - strand, within BT2749 at 3407.079 kb on - strand, within BT2749 at 3407.103 kb on - strand, within BT2749 at 3407.135 kb on - strand, within BT2749 at 3407.250 kb on + strand, within BT2749 at 3407.257 kb on + strand, within BT2749 at 3407.281 kb on + strand, within BT2749 at 3407.284 kb on + strand, within BT2749 at 3407.284 kb on + strand, within BT2749
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 3,404,801 - -1.2 3,406,324 - -1.8 3,406,324 - +1.4 3,406,333 - -1.8 3,406,416 + -0.2 3,406,416 + -1.2 3,406,417 - -0.2 3,406,450 - -4.0 3,406,452 - -3.8 3,406,452 - -3.9 3,406,455 - -2.9 3,406,542 - -1.9 3,406,657 + BT2749 0.12 +0.8 3,406,657 + BT2749 0.12 -1.8 3,406,658 - BT2749 0.12 -1.8 3,406,658 - BT2749 0.12 -2.5 3,406,711 + BT2749 0.15 -1.9 3,406,716 + BT2749 0.15 -2.1 3,406,733 + BT2749 0.16 -3.2 3,406,750 + BT2749 0.16 -2.8 3,406,759 + BT2749 0.17 -1.2 3,406,818 - BT2749 0.20 -0.6 3,406,896 + BT2749 0.24 -2.8 3,406,897 - BT2749 0.24 -3.3 3,406,898 + BT2749 0.24 -2.2 3,406,898 + BT2749 0.24 -1.8 3,406,898 + BT2749 0.24 -1.4 3,406,899 - BT2749 0.24 +0.8 3,406,900 + BT2749 0.24 -1.2 3,406,900 + BT2749 0.24 -2.1 3,406,901 - BT2749 0.24 +1.0 3,406,901 - BT2749 0.24 -0.2 3,406,901 - BT2749 0.24 +0.8 3,406,918 + BT2749 0.25 -2.2 3,406,924 + BT2749 0.25 -1.8 3,406,983 - BT2749 0.28 -1.2 3,407,079 - BT2749 0.32 -1.2 3,407,103 - BT2749 0.34 -3.0 3,407,135 - BT2749 0.35 -1.2 3,407,250 + BT2749 0.41 -2.3 3,407,257 + BT2749 0.41 -0.6 3,407,281 + BT2749 0.42 -1.8 3,407,284 + BT2749 0.42 -0.8 3,407,284 + BT2749 0.42 -1.8
Or see this region's nucleotide sequence