Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2541 and BT2542 are separated by 7 nucleotides BT2542 and BT2543 are separated by 10 nucleotides
BT2541: BT2541 - putative calcium-transporting ATPase (NCBI ptt file), at 3,173,813 to 3,176,503
BT2541
BT2542: BT2542 - putative haloacid dehalogenase-like hydrolase (NCBI ptt file), at 3,176,511 to 3,177,134
BT2542
BT2543: BT2543 - riboflavin biosynthesis protein ribF, putative riboflavin kinase (NCBI ptt file), at 3,177,145 to 3,178,107
BT2543
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3175.555 kb on + strand, within BT2541 at 3175.876 kb on + strand, within BT2541 at 3175.899 kb on + strand, within BT2541 at 3175.923 kb on + strand, within BT2541 at 3175.941 kb on + strand, within BT2541 at 3175.941 kb on + strand, within BT2541 at 3176.014 kb on + strand, within BT2541 at 3176.095 kb on + strand, within BT2541 at 3176.095 kb on + strand, within BT2541 at 3176.122 kb on + strand, within BT2541 at 3176.127 kb on + strand, within BT2541 at 3176.171 kb on + strand, within BT2541 at 3176.197 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.431 kb on + strand at 3176.431 kb on + strand at 3176.488 kb on + strand at 3176.528 kb on + strand at 3176.557 kb on + strand at 3176.585 kb on + strand, within BT2542 at 3176.656 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.919 kb on + strand, within BT2542 at 3176.934 kb on + strand, within BT2542 at 3176.934 kb on + strand, within BT2542 at 3176.977 kb on + strand, within BT2542 at 3177.053 kb on + strand, within BT2542 at 3177.074 kb on + strand at 3177.140 kb on + strand at 3177.144 kb on + strand at 3178.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 3,175,555 + BT2541 0.65 -1.1 3,175,876 + BT2541 0.77 -1.5 3,175,899 + BT2541 0.78 +0.1 3,175,923 + BT2541 0.78 -0.9 3,175,941 + BT2541 0.79 -2.4 3,175,941 + BT2541 0.79 -1.1 3,176,014 + BT2541 0.82 -3.1 3,176,095 + BT2541 0.85 -0.1 3,176,095 + BT2541 0.85 -2.1 3,176,122 + BT2541 0.86 +0.5 3,176,127 + BT2541 0.86 -1.1 3,176,171 + BT2541 0.88 -0.1 3,176,197 + BT2541 0.89 -3.6 3,176,209 + BT2541 0.89 -0.7 3,176,209 + BT2541 0.89 -2.3 3,176,209 + BT2541 0.89 +1.5 3,176,431 + -3.3 3,176,431 + -3.2 3,176,488 + -1.1 3,176,528 + -0.1 3,176,557 + -1.7 3,176,585 + BT2542 0.12 -1.1 3,176,656 + BT2542 0.23 -0.1 3,176,900 + BT2542 0.62 -1.0 3,176,900 + BT2542 0.62 -0.7 3,176,900 + BT2542 0.62 -1.7 3,176,919 + BT2542 0.65 -1.1 3,176,934 + BT2542 0.68 -1.1 3,176,934 + BT2542 0.68 -0.4 3,176,977 + BT2542 0.75 -0.9 3,177,053 + BT2542 0.87 -1.7 3,177,074 + -2.4 3,177,140 + -1.7 3,177,144 + -0.1 3,178,082 + -1.1
Or see this region's nucleotide sequence