Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2491 and BT2492 are separated by 7 nucleotides BT2492 and BT2493 are separated by 170 nucleotides BT2493 and BT2494 are separated by 178 nucleotides
BT2491: BT2491 - hypothetical protein (NCBI ptt file), at 3,114,126 to 3,114,707
BT2491
BT2492: BT2492 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 3,114,715 to 3,115,191
BT2492
BT2493: BT2493 - ROK family transcriptional repressor, with glucokinase domain (NCBI ptt file), at 3,115,362 to 3,116,342
BT2493
BT2494: BT2494 - ABC transporter, ATP-binding protein (NCBI ptt file), at 3,116,521 to 3,117,237
BT2494
Position (kb)
3115
3116
3117 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3114.723 kb on + strand at 3114.748 kb on - strand at 3114.790 kb on + strand, within BT2492 at 3114.795 kb on + strand, within BT2492 at 3114.795 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.835 kb on + strand, within BT2492 at 3114.836 kb on - strand, within BT2492 at 3114.850 kb on - strand at 3114.882 kb on - strand, within BT2492 at 3114.904 kb on - strand, within BT2492 at 3114.991 kb on - strand, within BT2492 at 3114.991 kb on - strand, within BT2492 at 3115.001 kb on - strand, within BT2492 at 3115.021 kb on + strand, within BT2492 at 3115.023 kb on + strand, within BT2492 at 3115.023 kb on + strand, within BT2492 at 3115.026 kb on + strand, within BT2492 at 3115.044 kb on + strand, within BT2492 at 3115.146 kb on + strand at 3115.227 kb on + strand at 3115.228 kb on - strand at 3115.268 kb on + strand at 3115.273 kb on - strand at 3116.395 kb on - strand at 3116.395 kb on - strand at 3116.508 kb on - strand at 3116.531 kb on + strand at 3116.661 kb on - strand, within BT2494 at 3116.661 kb on - strand, within BT2494 at 3116.666 kb on + strand, within BT2494 at 3116.669 kb on + strand, within BT2494 at 3116.701 kb on + strand, within BT2494 at 3116.704 kb on + strand, within BT2494 at 3116.718 kb on - strand, within BT2494 at 3116.722 kb on - strand, within BT2494 at 3116.722 kb on - strand, within BT2494 at 3116.756 kb on - strand, within BT2494 at 3116.845 kb on + strand, within BT2494 at 3116.846 kb on - strand, within BT2494 at 3116.855 kb on + strand, within BT2494 at 3117.240 kb on - strand at 3117.242 kb on + strand at 3117.243 kb on - strand at 3117.247 kb on - strand at 3117.250 kb on - strand at 3117.293 kb on - strand at 3117.297 kb on - strand at 3117.299 kb on + strand at 3117.303 kb on + strand at 3117.311 kb on - strand at 3117.325 kb on - strand at 3117.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 3,114,723 + -1.1 3,114,748 - -0.1 3,114,790 + BT2492 0.16 -1.4 3,114,795 + BT2492 0.17 -0.1 3,114,795 + BT2492 0.17 -3.8 3,114,835 + BT2492 0.25 +0.9 3,114,835 + BT2492 0.25 -1.1 3,114,835 + BT2492 0.25 +1.5 3,114,836 - BT2492 0.25 +2.5 3,114,850 - +0.3 3,114,882 - BT2492 0.35 -2.1 3,114,904 - BT2492 0.40 -1.7 3,114,991 - BT2492 0.58 -1.9 3,114,991 - BT2492 0.58 -0.1 3,115,001 - BT2492 0.60 -2.7 3,115,021 + BT2492 0.64 +1.5 3,115,023 + BT2492 0.65 -2.4 3,115,023 + BT2492 0.65 -0.1 3,115,026 + BT2492 0.65 -1.8 3,115,044 + BT2492 0.69 +1.9 3,115,146 + -0.1 3,115,227 + +1.5 3,115,228 - -0.7 3,115,268 + -0.7 3,115,273 - +0.9 3,116,395 - -0.8 3,116,395 - -2.7 3,116,508 - -2.3 3,116,531 + -0.6 3,116,661 - BT2494 0.20 -1.3 3,116,661 - BT2494 0.20 -1.7 3,116,666 + BT2494 0.20 -1.1 3,116,669 + BT2494 0.21 -2.5 3,116,701 + BT2494 0.25 -0.8 3,116,704 + BT2494 0.26 -0.1 3,116,718 - BT2494 0.27 +1.9 3,116,722 - BT2494 0.28 -2.2 3,116,722 - BT2494 0.28 -0.1 3,116,756 - BT2494 0.33 +0.2 3,116,845 + BT2494 0.45 -0.1 3,116,846 - BT2494 0.45 -1.5 3,116,855 + BT2494 0.47 -2.5 3,117,240 - -0.1 3,117,242 + -0.7 3,117,243 - -1.0 3,117,247 - -1.1 3,117,250 - +1.5 3,117,293 - -0.1 3,117,297 - -1.9 3,117,299 + -0.9 3,117,303 + -2.5 3,117,311 - -0.1 3,117,325 - -0.6 3,117,335 - -0.7
Or see this region's nucleotide sequence