Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2003 and BT2004 are separated by 12 nucleotides BT2004 and BT2005 overlap by 4 nucleotides BT2005 and BT2006 are separated by 129 nucleotides BT2006 and BT2007 are separated by 121 nucleotides
BT2003: BT2003 - putative membrane peptidase (NCBI ptt file), at 2,519,858 to 2,520,715
BT2003
BT2004: BT2004 - RimM protein, required for 16S rRNA processing (NCBI ptt file), at 2,520,728 to 2,521,267
BT2004
BT2005: BT2005 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI ptt file), at 2,521,264 to 2,522,568
BT2005
BT2006: BT2006 - hypothetical protein (NCBI ptt file), at 2,522,698 to 2,523,312
BT2006
BT2007: BT2007 - conserved hypothetical protein, putative glycoprotease (NCBI ptt file), at 2,523,434 to 2,524,129
BT2007
Position (kb)
2521
2522
2523 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2520.641 kb on - strand at 2522.594 kb on - strand at 2522.597 kb on - strand at 2522.618 kb on - strand at 2522.681 kb on - strand at 2523.169 kb on - strand, within BT2006 at 2523.169 kb on - strand, within BT2006 at 2523.169 kb on - strand, within BT2006 at 2523.196 kb on - strand, within BT2006 at 2523.257 kb on - strand at 2523.306 kb on - strand at 2523.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 2,520,641 - -2.8 2,522,594 - -1.1 2,522,597 - -2.8 2,522,618 - -2.0 2,522,681 - -2.3 2,523,169 - BT2006 0.77 -3.8 2,523,169 - BT2006 0.77 -0.0 2,523,169 - BT2006 0.77 -2.8 2,523,196 - BT2006 0.81 -1.0 2,523,257 - +0.8 2,523,306 - -2.6 2,523,405 - -2.6
Or see this region's nucleotide sequence