Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1333 and BT1334 are separated by 110 nucleotides BT1334 and BT1335 are separated by 131 nucleotides
BT1333: BT1333 - putative dihydropyrimidine dehydrogenase [NADP+] precursor (NCBI ptt file), at 1,657,781 to 1,658,761
BT1333
BT1334: BT1334 - PhoH-like protein (NCBI ptt file), at 1,658,872 to 1,660,197
BT1334
BT1335: BT1335 - folylpolyglutamate synthase (NCBI ptt file), at 1,660,329 to 1,661,804
BT1335
Position (kb)
1658
1659
1660
1661 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1657.873 kb on + strand at 1657.908 kb on + strand, within BT1333 at 1657.908 kb on + strand, within BT1333 at 1657.937 kb on - strand, within BT1333 at 1657.991 kb on + strand, within BT1333 at 1658.003 kb on + strand, within BT1333 at 1658.003 kb on + strand, within BT1333 at 1658.017 kb on + strand, within BT1333 at 1658.030 kb on + strand, within BT1333 at 1658.054 kb on - strand, within BT1333 at 1658.054 kb on - strand, within BT1333 at 1658.068 kb on + strand, within BT1333 at 1658.079 kb on + strand, within BT1333 at 1658.133 kb on + strand, within BT1333 at 1658.183 kb on + strand, within BT1333 at 1658.190 kb on + strand, within BT1333 at 1658.195 kb on + strand, within BT1333 at 1658.197 kb on + strand, within BT1333 at 1658.202 kb on - strand, within BT1333 at 1658.202 kb on - strand, within BT1333 at 1658.242 kb on + strand, within BT1333 at 1658.243 kb on - strand, within BT1333 at 1658.322 kb on + strand, within BT1333 at 1658.322 kb on + strand, within BT1333 at 1658.322 kb on + strand, within BT1333 at 1658.322 kb on + strand, within BT1333 at 1658.322 kb on + strand, within BT1333 at 1658.323 kb on - strand, within BT1333 at 1658.324 kb on + strand, within BT1333 at 1658.325 kb on - strand, within BT1333 at 1658.325 kb on - strand, within BT1333 at 1658.533 kb on - strand, within BT1333 at 1658.578 kb on + strand, within BT1333 at 1658.578 kb on + strand, within BT1333 at 1658.591 kb on - strand, within BT1333 at 1658.704 kb on + strand at 1658.710 kb on + strand at 1658.711 kb on - strand at 1658.722 kb on + strand at 1658.793 kb on + strand at 1658.794 kb on - strand at 1658.814 kb on + strand at 1658.825 kb on + strand at 1658.908 kb on - strand at 1658.944 kb on - strand at 1658.970 kb on + strand at 1659.037 kb on + strand, within BT1334 at 1659.202 kb on - strand, within BT1334 at 1659.275 kb on + strand, within BT1334 at 1659.276 kb on - strand, within BT1334 at 1659.276 kb on - strand, within BT1334 at 1659.511 kb on - strand, within BT1334 at 1659.515 kb on + strand, within BT1334 at 1659.575 kb on - strand, within BT1334 at 1659.630 kb on + strand, within BT1334 at 1659.671 kb on + strand, within BT1334 at 1659.713 kb on + strand, within BT1334 at 1659.731 kb on - strand, within BT1334 at 1659.827 kb on - strand, within BT1334 at 1660.094 kb on - strand at 1660.158 kb on + strand at 1660.221 kb on + strand at 1660.323 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 1,657,873 + -2.9 1,657,908 + BT1333 0.13 +0.1 1,657,908 + BT1333 0.13 -1.0 1,657,937 - BT1333 0.16 +1.7 1,657,991 + BT1333 0.21 +0.1 1,658,003 + BT1333 0.23 -1.9 1,658,003 + BT1333 0.23 -0.7 1,658,017 + BT1333 0.24 +0.1 1,658,030 + BT1333 0.25 +1.1 1,658,054 - BT1333 0.28 -2.2 1,658,054 - BT1333 0.28 +0.1 1,658,068 + BT1333 0.29 -1.9 1,658,079 + BT1333 0.30 -1.9 1,658,133 + BT1333 0.36 -0.9 1,658,183 + BT1333 0.41 -0.1 1,658,190 + BT1333 0.42 -2.2 1,658,195 + BT1333 0.42 -0.4 1,658,197 + BT1333 0.42 -0.3 1,658,202 - BT1333 0.43 -0.4 1,658,202 - BT1333 0.43 +0.1 1,658,242 + BT1333 0.47 -3.4 1,658,243 - BT1333 0.47 -1.0 1,658,322 + BT1333 0.55 +0.7 1,658,322 + BT1333 0.55 +0.1 1,658,322 + BT1333 0.55 -0.9 1,658,322 + BT1333 0.55 +0.1 1,658,322 + BT1333 0.55 -1.3 1,658,323 - BT1333 0.55 +0.1 1,658,324 + BT1333 0.55 -1.9 1,658,325 - BT1333 0.55 +3.1 1,658,325 - BT1333 0.55 +0.1 1,658,533 - BT1333 0.77 -0.6 1,658,578 + BT1333 0.81 -0.2 1,658,578 + BT1333 0.81 -2.4 1,658,591 - BT1333 0.83 +1.1 1,658,704 + -2.1 1,658,710 + -3.3 1,658,711 - -1.9 1,658,722 + -0.2 1,658,793 + -0.9 1,658,794 - -1.4 1,658,814 + +1.1 1,658,825 + -1.9 1,658,908 - -0.9 1,658,944 - -0.6 1,658,970 + -1.4 1,659,037 + BT1334 0.12 -1.1 1,659,202 - BT1334 0.25 -1.4 1,659,275 + BT1334 0.30 -1.3 1,659,276 - BT1334 0.30 -1.3 1,659,276 - BT1334 0.30 -1.4 1,659,511 - BT1334 0.48 +0.1 1,659,515 + BT1334 0.48 -1.9 1,659,575 - BT1334 0.53 +0.1 1,659,630 + BT1334 0.57 -1.8 1,659,671 + BT1334 0.60 +0.1 1,659,713 + BT1334 0.63 -0.9 1,659,731 - BT1334 0.65 +0.1 1,659,827 - BT1334 0.72 +0.1 1,660,094 - -1.6 1,660,158 + -0.9 1,660,221 + -2.4 1,660,323 + -3.0
Or see this region's nucleotide sequence