Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0894 and BT0895 are separated by 59 nucleotides BT0895 and tRNA-Arg are separated by 540 nucleotides tRNA-Arg and tRNA-Arg are separated by 20 nucleotides tRNA-Arg and tRNA-Arg are separated by 20 nucleotides tRNA-Arg and BT0896 are separated by 79 nucleotides
BT0894: BT0894 - DNA ligase (NCBI ptt file), at 1,096,350 to 1,098,350
BT0894
BT0895: BT0895 - dihydrodipicolinate synthase (NCBI ptt file), at 1,098,410 to 1,099,303
BT0895
BT_t14: tRNA-Arg - tRNA-Arg (RefSeq), at 1,099,844 to 1,099,920
tRNA-Arg
BT_t15: tRNA-Arg - tRNA-Arg (RefSeq), at 1,099,941 to 1,100,014
tRNA-Arg
BT_t16: tRNA-Arg - tRNA-Arg (RefSeq), at 1,100,035 to 1,100,108
tRNA-Arg
BT0896: BT0896 - putative patatin-like phospholipase (NCBI ptt file), at 1,100,188 to 1,102,446
BT0896
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-2
-1
0
1 at 1099.270 kb on + strand at 1099.320 kb on - strand at 1099.372 kb on - strand at 1099.479 kb on + strand at 1099.483 kb on - strand at 1099.499 kb on + strand at 1099.500 kb on - strand at 1099.508 kb on - strand at 1099.662 kb on + strand at 1099.669 kb on + strand at 1099.669 kb on + strand at 1099.669 kb on + strand at 1099.705 kb on + strand at 1099.926 kb on - strand at 1099.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 1,099,270 + -0.9 1,099,320 - -1.2 1,099,372 - -2.2 1,099,479 + -1.9 1,099,483 - -0.6 1,099,499 + -1.2 1,099,500 - -0.9 1,099,508 - -0.9 1,099,662 + -2.4 1,099,669 + +0.1 1,099,669 + +0.1 1,099,669 + -1.9 1,099,705 + -1.4 1,099,926 - +0.1 1,099,936 - -2.4
Or see this region's nucleotide sequence