Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1624 and Psest_1625 are separated by 57 nucleotides Psest_1625 and Psest_1626 are separated by 45 nucleotides Psest_1626 and Psest_1627 are separated by 150 nucleotides
Psest_1624: Psest_1624 - Transcriptional regulator, at 1,727,324 to 1,728,232
_1624
Psest_1625: Psest_1625 - hypothetical protein, at 1,728,290 to 1,728,610
_1625
Psest_1626: Psest_1626 - Predicted integral membrane protein, at 1,728,656 to 1,729,093
_1626
Psest_1627: Psest_1627 - Superfamily II DNA and RNA helicases, at 1,729,244 to 1,730,575
_1627
Position (kb)
1728
1729
1730 Strain fitness (log2 ratio)
-2
-1
0
1 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.246 kb on - strand at 1728.248 kb on - strand at 1728.272 kb on - strand at 1728.351 kb on - strand, within Psest_1625 at 1728.357 kb on + strand, within Psest_1625 at 1728.358 kb on - strand, within Psest_1625 at 1728.447 kb on - strand, within Psest_1625 at 1728.608 kb on + strand at 1728.609 kb on - strand at 1728.665 kb on + strand at 1728.666 kb on - strand at 1728.666 kb on - strand at 1728.744 kb on + strand, within Psest_1626 at 1728.754 kb on - strand, within Psest_1626 at 1728.933 kb on + strand, within Psest_1626 at 1729.038 kb on + strand, within Psest_1626 at 1729.039 kb on - strand, within Psest_1626 at 1729.039 kb on - strand, within Psest_1626 at 1729.055 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.216 kb on + strand at 1729.217 kb on - strand at 1729.236 kb on + strand at 1729.237 kb on - strand at 1729.362 kb on + strand at 1729.362 kb on + strand at 1729.362 kb on + strand at 1729.363 kb on - strand at 1729.970 kb on + strand, within Psest_1627 at 1729.970 kb on + strand, within Psest_1627 at 1729.970 kb on + strand, within Psest_1627 at 1729.971 kb on - strand, within Psest_1627 at 1729.971 kb on - strand, within Psest_1627 at 1729.971 kb on - strand, within Psest_1627 at 1729.971 kb on - strand, within Psest_1627
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,728,117 + Psest_1624 0.87 +0.0 1,728,117 + Psest_1624 0.87 -2.4 1,728,117 + Psest_1624 0.87 -0.1 1,728,117 + Psest_1624 0.87 -0.8 1,728,117 + Psest_1624 0.87 +0.3 1,728,117 + Psest_1624 0.87 -0.0 1,728,118 - Psest_1624 0.87 -1.0 1,728,118 - Psest_1624 0.87 +0.2 1,728,118 - Psest_1624 0.87 -0.7 1,728,118 - Psest_1624 0.87 +1.3 1,728,118 - Psest_1624 0.87 +1.0 1,728,118 - Psest_1624 0.87 -0.2 1,728,246 - -0.9 1,728,248 - -0.5 1,728,272 - -0.6 1,728,351 - Psest_1625 0.19 -1.0 1,728,357 + Psest_1625 0.21 -0.1 1,728,358 - Psest_1625 0.21 +0.2 1,728,447 - Psest_1625 0.49 -0.6 1,728,608 + -0.0 1,728,609 - -0.3 1,728,665 + +0.9 1,728,666 - -0.5 1,728,666 - +0.8 1,728,744 + Psest_1626 0.20 -0.5 1,728,754 - Psest_1626 0.22 -0.3 1,728,933 + Psest_1626 0.63 -2.4 1,729,038 + Psest_1626 0.87 -0.7 1,729,039 - Psest_1626 0.87 +0.4 1,729,039 - Psest_1626 0.87 -0.6 1,729,055 + -2.7 1,729,206 + +0.3 1,729,206 + +0.1 1,729,206 + +0.1 1,729,206 + -1.0 1,729,207 - -1.0 1,729,207 - +0.2 1,729,207 - -0.7 1,729,207 - -0.6 1,729,207 - +0.6 1,729,216 + +1.5 1,729,217 - +0.2 1,729,236 + +0.2 1,729,237 - -0.6 1,729,362 + -1.2 1,729,362 + -0.4 1,729,362 + +0.2 1,729,363 - -0.2 1,729,970 + Psest_1627 0.55 +0.7 1,729,970 + Psest_1627 0.55 -1.1 1,729,970 + Psest_1627 0.55 -1.1 1,729,971 - Psest_1627 0.55 -1.4 1,729,971 - Psest_1627 0.55 -2.0 1,729,971 - Psest_1627 0.55 -0.3 1,729,971 - Psest_1627 0.55 -0.8
Or see this region's nucleotide sequence