Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3870 and Psest_3871 are separated by 97 nucleotides Psest_3871 and Psest_3872 are separated by 28 nucleotides Psest_3872 and Psest_3873 are separated by 169 nucleotides
Psest_3870: Psest_3870 - Uncharacterized protein conserved in bacteria, at 4,041,094 to 4,042,014
_3870
Psest_3871: Psest_3871 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 4,042,112 to 4,042,777
_3871
Psest_3872: Psest_3872 - FAD/FMN-containing dehydrogenases, at 4,042,806 to 4,044,224
_3872
Psest_3873: Psest_3873 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 4,044,394 to 4,045,629
_3873
Position (kb)
4042
4043
4044
4045 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4042.047 kb on + strand at 4042.047 kb on + strand at 4042.047 kb on + strand at 4042.047 kb on + strand at 4042.048 kb on - strand at 4042.048 kb on - strand at 4042.048 kb on - strand at 4042.160 kb on + strand at 4042.160 kb on + strand at 4042.570 kb on - strand, within Psest_3871 at 4042.619 kb on + strand, within Psest_3871 at 4042.698 kb on + strand, within Psest_3871 at 4042.698 kb on + strand, within Psest_3871 at 4042.699 kb on - strand, within Psest_3871 at 4042.699 kb on - strand, within Psest_3871 at 4042.699 kb on - strand, within Psest_3871 at 4042.699 kb on - strand, within Psest_3871 at 4042.758 kb on + strand at 4042.759 kb on - strand at 4042.759 kb on - strand at 4042.759 kb on - strand at 4042.759 kb on - strand at 4042.759 kb on - strand at 4042.759 kb on - strand at 4042.876 kb on + strand at 4042.876 kb on + strand at 4042.877 kb on - strand at 4042.877 kb on - strand at 4042.912 kb on + strand at 4042.912 kb on + strand at 4042.963 kb on + strand, within Psest_3872 at 4042.963 kb on + strand, within Psest_3872 at 4043.188 kb on + strand, within Psest_3872 at 4043.189 kb on - strand, within Psest_3872 at 4043.189 kb on - strand, within Psest_3872 at 4043.189 kb on - strand, within Psest_3872 at 4043.219 kb on + strand, within Psest_3872 at 4043.219 kb on + strand, within Psest_3872 at 4043.220 kb on - strand, within Psest_3872 at 4043.220 kb on - strand, within Psest_3872 at 4043.220 kb on - strand, within Psest_3872 at 4043.220 kb on - strand, within Psest_3872 at 4043.220 kb on - strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.221 kb on + strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.222 kb on - strand, within Psest_3872 at 4043.923 kb on + strand, within Psest_3872 at 4043.923 kb on + strand, within Psest_3872 at 4043.923 kb on + strand, within Psest_3872 at 4043.924 kb on - strand, within Psest_3872 at 4043.924 kb on - strand, within Psest_3872 at 4044.115 kb on + strand at 4044.215 kb on + strand at 4044.215 kb on + strand at 4044.215 kb on + strand at 4044.215 kb on + strand at 4044.277 kb on + strand at 4044.277 kb on + strand at 4044.277 kb on + strand at 4044.277 kb on + strand at 4044.278 kb on - strand at 4044.278 kb on - strand at 4044.278 kb on - strand at 4044.278 kb on - strand at 4044.278 kb on - strand at 4044.282 kb on + strand at 4044.287 kb on + strand at 4044.287 kb on + strand at 4044.384 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,042,047 + +0.6 4,042,047 + -0.5 4,042,047 + -0.1 4,042,047 + -0.9 4,042,048 - +0.9 4,042,048 - +0.1 4,042,048 - +0.1 4,042,160 + +0.1 4,042,160 + +1.0 4,042,570 - Psest_3871 0.69 -1.2 4,042,619 + Psest_3871 0.76 -0.6 4,042,698 + Psest_3871 0.88 -1.6 4,042,698 + Psest_3871 0.88 +0.2 4,042,699 - Psest_3871 0.88 -0.5 4,042,699 - Psest_3871 0.88 -0.1 4,042,699 - Psest_3871 0.88 -1.6 4,042,699 - Psest_3871 0.88 -0.2 4,042,758 + -1.9 4,042,759 - -0.5 4,042,759 - -0.8 4,042,759 - -1.5 4,042,759 - -0.1 4,042,759 - +0.5 4,042,759 - -0.1 4,042,876 + -1.3 4,042,876 + -0.6 4,042,877 - -2.5 4,042,877 - -2.1 4,042,912 + -1.9 4,042,912 + -0.2 4,042,963 + Psest_3872 0.11 -1.8 4,042,963 + Psest_3872 0.11 -2.5 4,043,188 + Psest_3872 0.27 -2.9 4,043,189 - Psest_3872 0.27 -1.1 4,043,189 - Psest_3872 0.27 -1.9 4,043,189 - Psest_3872 0.27 -0.8 4,043,219 + Psest_3872 0.29 -0.5 4,043,219 + Psest_3872 0.29 -0.9 4,043,220 - Psest_3872 0.29 -1.2 4,043,220 - Psest_3872 0.29 -2.2 4,043,220 - Psest_3872 0.29 -0.6 4,043,220 - Psest_3872 0.29 -1.0 4,043,220 - Psest_3872 0.29 -2.1 4,043,221 + Psest_3872 0.29 -2.1 4,043,221 + Psest_3872 0.29 -1.4 4,043,221 + Psest_3872 0.29 -1.4 4,043,221 + Psest_3872 0.29 -1.0 4,043,221 + Psest_3872 0.29 -1.9 4,043,221 + Psest_3872 0.29 -2.5 4,043,221 + Psest_3872 0.29 -1.2 4,043,221 + Psest_3872 0.29 -1.6 4,043,222 - Psest_3872 0.29 -3.8 4,043,222 - Psest_3872 0.29 -2.0 4,043,222 - Psest_3872 0.29 -0.6 4,043,222 - Psest_3872 0.29 -1.0 4,043,222 - Psest_3872 0.29 -1.9 4,043,222 - Psest_3872 0.29 -0.0 4,043,222 - Psest_3872 0.29 -2.3 4,043,222 - Psest_3872 0.29 -1.9 4,043,222 - Psest_3872 0.29 -2.7 4,043,923 + Psest_3872 0.79 -2.5 4,043,923 + Psest_3872 0.79 -2.0 4,043,923 + Psest_3872 0.79 -0.8 4,043,924 - Psest_3872 0.79 -0.9 4,043,924 - Psest_3872 0.79 -0.7 4,044,115 + -1.9 4,044,215 + -3.0 4,044,215 + -1.9 4,044,215 + -1.7 4,044,215 + -2.2 4,044,277 + -0.3 4,044,277 + -0.0 4,044,277 + -1.1 4,044,277 + -0.4 4,044,278 - -3.7 4,044,278 - -1.6 4,044,278 - -2.6 4,044,278 - -1.4 4,044,278 - +1.5 4,044,282 + -2.0 4,044,287 + -0.7 4,044,287 + -1.2 4,044,384 + -1.6
Or see this region's nucleotide sequence