Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0105

Experiment: Ethanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0103 and Psest_0104 are separated by 2 nucleotidesPsest_0104 and Psest_0105 overlap by 8 nucleotidesPsest_0105 and Psest_0106 overlap by 4 nucleotidesPsest_0106 and Psest_0107 are separated by 72 nucleotides Psest_0103: Psest_0103 - triphosphoribosyl-dephospho-CoA synthase MdcB, at 103,017 to 103,895 _0103 Psest_0104: Psest_0104 - malonate decarboxylase acyl carrier protein, at 103,898 to 104,197 _0104 Psest_0105: Psest_0105 - malonate decarboxylase, beta subunit, at 104,190 to 105,056 _0105 Psest_0106: Psest_0106 - malonate decarboxylase, gamma subunit, at 105,053 to 105,838 _0106 Psest_0107: Psest_0107 - malonate decarboxylase holo-[acyl-carrier-protein] synthase, at 105,911 to 106,528 _0107 Position (kb) 104 105 106Strain fitness (log2 ratio) -1 0 1 2 3at 103.327 kb on + strand, within Psest_0103at 103.327 kb on + strand, within Psest_0103at 103.328 kb on - strand, within Psest_0103at 103.328 kb on - strand, within Psest_0103at 103.466 kb on + strand, within Psest_0103at 103.466 kb on + strand, within Psest_0103at 104.640 kb on + strand, within Psest_0105at 104.640 kb on + strand, within Psest_0105at 104.718 kb on + strand, within Psest_0105at 104.719 kb on - strand, within Psest_0105at 104.719 kb on - strand, within Psest_0105at 104.797 kb on - strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 105.155 kb on - strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.165 kb on - strand, within Psest_0106at 105.165 kb on - strand, within Psest_0106at 105.325 kb on - strand, within Psest_0106at 105.339 kb on - strand, within Psest_0106at 105.621 kb on - strand, within Psest_0106at 105.635 kb on + strand, within Psest_0106at 105.635 kb on + strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.908 kb on + strandat 105.908 kb on + strandat 105.908 kb on + strandat 105.909 kb on - strandat 105.909 kb on - strandat 105.909 kb on - strandat 105.909 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ethanol (C)
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103,327 + Psest_0103 0.35 +2.3
103,327 + Psest_0103 0.35 +0.6
103,328 - Psest_0103 0.35 -0.4
103,328 - Psest_0103 0.35 +0.0
103,466 + Psest_0103 0.51 +1.7
103,466 + Psest_0103 0.51 +1.2
104,640 + Psest_0105 0.52 +1.9
104,640 + Psest_0105 0.52 +1.1
104,718 + Psest_0105 0.61 +1.3
104,719 - Psest_0105 0.61 -1.7
104,719 - Psest_0105 0.61 +0.7
104,797 - Psest_0105 0.70 -0.5
104,874 + Psest_0105 0.79 +2.2
104,874 + Psest_0105 0.79 +2.0
104,874 + Psest_0105 0.79 +1.7
104,874 + Psest_0105 0.79 +2.2
104,874 + Psest_0105 0.79 +2.7
104,874 + Psest_0105 0.79 +2.2
104,874 + Psest_0105 0.79 +1.4
104,874 + Psest_0105 0.79 +1.9
104,875 - Psest_0105 0.79 +0.7
104,875 - Psest_0105 0.79 +0.8
104,875 - Psest_0105 0.79 +0.2
105,155 - Psest_0106 0.13 -0.4
105,164 + Psest_0106 0.14 +2.8
105,164 + Psest_0106 0.14 +2.4
105,164 + Psest_0106 0.14 +2.0
105,165 - Psest_0106 0.14 +0.0
105,165 - Psest_0106 0.14 -0.4
105,325 - Psest_0106 0.35 -0.2
105,339 - Psest_0106 0.36 -0.2
105,621 - Psest_0106 0.72 +0.5
105,635 + Psest_0106 0.74 +2.9
105,635 + Psest_0106 0.74 +2.1
105,636 - Psest_0106 0.74 -1.3
105,636 - Psest_0106 0.74 +1.6
105,636 - Psest_0106 0.74 -0.3
105,636 - Psest_0106 0.74 +0.2
105,636 - Psest_0106 0.74 -1.0
105,908 + +1.2
105,908 + -0.4
105,908 + -0.3
105,909 - -1.6
105,909 - -0.8
105,909 - +0.6
105,909 - +0.1

Or see this region's nucleotide sequence