Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2983 and Psest_2984 are separated by 51 nucleotides Psest_2984 and Psest_2985 are separated by 70 nucleotides Psest_2985 and Psest_2986 are separated by 109 nucleotides Psest_2986 and Psest_2987 are separated by 62 nucleotides Psest_2987 and Psest_2988 are separated by 180 nucleotides Psest_2988 and Psest_2989 are separated by 112 nucleotides
Psest_2983: Psest_2983 - hypothetical protein, at 3,175,660 to 3,176,142
_2983
Psest_2984: Psest_2984 - tRNA_Arg_ACG, at 3,176,194 to 3,176,270
_2984
Psest_2985: Psest_2985 - tRNA_Arg_ACG, at 3,176,341 to 3,176,417
_2985
Psest_2986: Psest_2986 - tRNA_Ser_GCT, at 3,176,527 to 3,176,617
_2986
Psest_2987: Psest_2987 - carbon storage regulator (csrA), at 3,176,680 to 3,176,865
_2987
Psest_2988: Psest_2988 - aspartate kinase, monofunctional class, at 3,177,046 to 3,178,284
_2988
Psest_2989: Psest_2989 - alanine--tRNA ligase, at 3,178,397 to 3,181,021
_2989
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3176.245 kb on - strand, within Psest_2984 at 3176.263 kb on - strand at 3176.263 kb on - strand at 3176.263 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.273 kb on - strand at 3176.284 kb on - strand at 3176.284 kb on - strand at 3176.946 kb on - strand at 3176.946 kb on - strand at 3176.985 kb on - strand at 3176.985 kb on - strand at 3177.010 kb on - strand at 3177.010 kb on - strand at 3177.010 kb on - strand at 3177.120 kb on - strand at 3177.120 kb on - strand at 3177.120 kb on - strand at 3177.323 kb on - strand, within Psest_2988 at 3177.323 kb on - strand, within Psest_2988 at 3177.342 kb on - strand, within Psest_2988 at 3177.469 kb on - strand, within Psest_2988 at 3177.469 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.621 kb on - strand, within Psest_2988 at 3177.750 kb on - strand, within Psest_2988 at 3177.750 kb on - strand, within Psest_2988 at 3177.750 kb on - strand, within Psest_2988 at 3177.750 kb on - strand, within Psest_2988 at 3177.750 kb on - strand at 3177.771 kb on - strand, within Psest_2988 at 3177.853 kb on - strand, within Psest_2988 at 3178.002 kb on - strand, within Psest_2988 at 3178.058 kb on - strand, within Psest_2988 at 3178.115 kb on - strand, within Psest_2988 at 3178.180 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.326 kb on - strand at 3178.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 3,176,245 - Psest_2984 0.66 +0.7 3,176,263 - +2.1 3,176,263 - +0.8 3,176,263 - +0.8 3,176,273 - -0.7 3,176,273 - +0.5 3,176,273 - -0.6 3,176,273 - -1.9 3,176,273 - -1.9 3,176,273 - +0.9 3,176,273 - -1.5 3,176,284 - -0.6 3,176,284 - -0.8 3,176,946 - +3.2 3,176,946 - +3.3 3,176,985 - +3.8 3,176,985 - +3.9 3,177,010 - +3.7 3,177,010 - +3.7 3,177,010 - +2.5 3,177,120 - -3.9 3,177,120 - -2.5 3,177,120 - -1.3 3,177,323 - Psest_2988 0.22 -4.0 3,177,323 - Psest_2988 0.22 -5.4 3,177,342 - Psest_2988 0.24 -2.9 3,177,469 - Psest_2988 0.34 -4.3 3,177,469 - Psest_2988 0.34 -1.8 3,177,621 - Psest_2988 0.46 -2.5 3,177,621 - Psest_2988 0.46 -3.7 3,177,621 - Psest_2988 0.46 -3.7 3,177,621 - Psest_2988 0.46 -2.0 3,177,621 - Psest_2988 0.46 -3.9 3,177,621 - Psest_2988 0.46 -4.7 3,177,750 - Psest_2988 0.57 -1.5 3,177,750 - Psest_2988 0.57 -3.7 3,177,750 - Psest_2988 0.57 -3.0 3,177,750 - Psest_2988 0.57 -4.4 3,177,750 - -1.3 3,177,771 - Psest_2988 0.59 -1.4 3,177,853 - Psest_2988 0.65 -3.2 3,178,002 - Psest_2988 0.77 -1.8 3,178,058 - Psest_2988 0.82 -1.3 3,178,115 - Psest_2988 0.86 -4.0 3,178,180 - -2.3 3,178,326 - +0.3 3,178,326 - +1.8 3,178,326 - +1.2 3,178,326 - +0.1 3,178,326 - +1.1 3,178,326 - +0.9 3,178,326 - -1.7 3,178,326 - +0.8 3,178,326 - +1.1 3,178,326 - +0.8 3,178,326 - +1.0 3,178,326 - +2.8 3,178,326 - +1.5 3,178,373 - +1.1
Or see this region's nucleotide sequence