Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1731

Experiment: L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1729 and Psest_1730 are separated by 40 nucleotidesPsest_1730 and Psest_1731 are separated by 1 nucleotidesPsest_1731 and Psest_1732 overlap by 1 nucleotidesPsest_1732 and Psest_1733 are separated by 12 nucleotides Psest_1729: Psest_1729 - flagellar motor switch protein FliM, at 1,835,251 to 1,836,222 _1729 Psest_1730: Psest_1730 - flagellar motor switch protein FliN, at 1,836,263 to 1,836,736 _1730 Psest_1731: Psest_1731 - flagellar biosynthetic protein FliO, at 1,836,738 to 1,837,157 _1731 Psest_1732: Psest_1732 - flagellar biosynthetic protein FliP, at 1,837,157 to 1,837,933 _1732 Psest_1733: Psest_1733 - flagellar biosynthetic protein FliQ, at 1,837,946 to 1,838,215 _1733 Position (kb) 1836 1837 1838Strain fitness (log2 ratio) -2 -1 0 1 2at 1835.782 kb on + strand, within Psest_1729at 1836.233 kb on + strandat 1836.234 kb on - strandat 1836.815 kb on + strand, within Psest_1731at 1837.174 kb on - strandat 1837.269 kb on + strand, within Psest_1732at 1837.276 kb on - strand, within Psest_1732at 1837.280 kb on - strand, within Psest_1732at 1837.280 kb on - strand, within Psest_1732at 1837.539 kb on - strand, within Psest_1732at 1837.539 kb on - strand, within Psest_1732at 1837.661 kb on - strand, within Psest_1732at 1837.661 kb on - strand, within Psest_1732at 1837.661 kb on - strand, within Psest_1732at 1837.931 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate (C)
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1,835,782 + Psest_1729 0.55 +2.4
1,836,233 + -0.1
1,836,234 - +0.9
1,836,815 + Psest_1731 0.18 +0.7
1,837,174 - +1.4
1,837,269 + Psest_1732 0.14 +0.2
1,837,276 - Psest_1732 0.15 +0.6
1,837,280 - Psest_1732 0.16 +1.0
1,837,280 - Psest_1732 0.16 -0.3
1,837,539 - Psest_1732 0.49 +0.5
1,837,539 - Psest_1732 0.49 -0.2
1,837,661 - Psest_1732 0.65 +0.9
1,837,661 - Psest_1732 0.65 +0.5
1,837,661 - Psest_1732 0.65 -1.8
1,837,931 + -0.4

Or see this region's nucleotide sequence