Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3099 and Psest_3100 are separated by 31 nucleotides Psest_3100 and Psest_3101 are separated by 349 nucleotides Psest_3101 and Psest_3102 overlap by 32 nucleotides Psest_3102 and Psest_3103 overlap by 17 nucleotides Psest_3103 and Psest_3104 are separated by 148 nucleotides
Psest_3099: Psest_3099 - Negative regulator of sigma E activity, at 3,277,824 to 3,278,402
_3099
Psest_3100: Psest_3100 - RNA polymerase sigma factor RpoE, at 3,278,434 to 3,279,015
_3100
Psest_3101: Psest_3101 - L-aspartate oxidase, at 3,279,365 to 3,280,981
_3101
Psest_3102: Psest_3102 - hypothetical protein, at 3,280,950 to 3,281,405
_3102
Psest_3103: Psest_3103 - Uncharacterized conserved protein, at 3,281,389 to 3,281,643
_3103
Psest_3104: Psest_3104 - folate-binding protein YgfZ, at 3,281,792 to 3,282,739
_3104
Position (kb)
3279
3280
3281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3278.467 kb on - strand at 3278.467 kb on - strand at 3278.467 kb on - strand at 3278.467 kb on - strand at 3278.467 kb on - strand at 3278.471 kb on - strand at 3278.471 kb on - strand at 3278.471 kb on - strand at 3278.551 kb on - strand, within Psest_3100 at 3278.551 kb on - strand, within Psest_3100 at 3278.551 kb on - strand, within Psest_3100 at 3278.551 kb on - strand, within Psest_3100 at 3278.551 kb on - strand, within Psest_3100 at 3278.576 kb on - strand, within Psest_3100 at 3278.576 kb on - strand, within Psest_3100 at 3278.584 kb on - strand, within Psest_3100 at 3278.697 kb on - strand, within Psest_3100 at 3278.697 kb on - strand, within Psest_3100 at 3278.773 kb on - strand, within Psest_3100 at 3278.800 kb on - strand, within Psest_3100 at 3278.800 kb on - strand, within Psest_3100 at 3278.802 kb on - strand, within Psest_3100 at 3278.802 kb on - strand, within Psest_3100 at 3278.841 kb on - strand, within Psest_3100 at 3278.849 kb on - strand, within Psest_3100 at 3278.849 kb on - strand, within Psest_3100 at 3278.849 kb on - strand, within Psest_3100 at 3278.931 kb on - strand, within Psest_3100 at 3279.077 kb on - strand at 3279.112 kb on - strand at 3279.130 kb on - strand at 3279.242 kb on - strand at 3279.297 kb on - strand at 3279.297 kb on - strand at 3279.309 kb on - strand at 3279.330 kb on + strand at 3279.331 kb on - strand at 3279.331 kb on - strand at 3279.332 kb on + strand at 3279.332 kb on + strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.333 kb on - strand at 3279.334 kb on + strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.335 kb on - strand at 3279.338 kb on - strand at 3279.338 kb on - strand at 3279.338 kb on - strand at 3279.378 kb on + strand at 3279.378 kb on + strand at 3279.379 kb on - strand at 3279.379 kb on - strand at 3279.379 kb on - strand at 3279.379 kb on - strand at 3279.390 kb on + strand at 3279.390 kb on + strand at 3279.390 kb on + strand at 3279.391 kb on - strand at 3279.498 kb on - strand at 3279.740 kb on - strand, within Psest_3101 at 3279.911 kb on + strand, within Psest_3101 at 3279.911 kb on + strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.912 kb on - strand, within Psest_3101 at 3279.996 kb on - strand, within Psest_3101 at 3279.996 kb on - strand, within Psest_3101 at 3279.996 kb on - strand, within Psest_3101 at 3280.110 kb on - strand, within Psest_3101 at 3280.292 kb on + strand, within Psest_3101 at 3280.350 kb on - strand, within Psest_3101 at 3280.350 kb on - strand, within Psest_3101 at 3280.368 kb on - strand, within Psest_3101 at 3280.368 kb on - strand, within Psest_3101 at 3280.418 kb on + strand, within Psest_3101 at 3280.419 kb on - strand, within Psest_3101 at 3280.419 kb on - strand, within Psest_3101 at 3280.419 kb on - strand, within Psest_3101 at 3280.419 kb on - strand, within Psest_3101 at 3280.475 kb on + strand, within Psest_3101 at 3280.476 kb on - strand, within Psest_3101 at 3280.476 kb on - strand, within Psest_3101 at 3280.604 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.713 kb on - strand, within Psest_3101 at 3280.797 kb on - strand, within Psest_3101 at 3280.797 kb on - strand, within Psest_3101 at 3280.797 kb on - strand, within Psest_3101 at 3280.805 kb on + strand, within Psest_3101 at 3280.805 kb on + strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.806 kb on - strand, within Psest_3101 at 3280.908 kb on - strand at 3280.908 kb on - strand at 3280.952 kb on - strand at 3281.045 kb on + strand, within Psest_3102 at 3281.045 kb on + strand, within Psest_3102 at 3281.045 kb on + strand, within Psest_3102 at 3281.045 kb on + strand, within Psest_3102 at 3281.046 kb on - strand, within Psest_3102 at 3281.046 kb on - strand, within Psest_3102 at 3281.302 kb on + strand, within Psest_3102 at 3281.302 kb on + strand, within Psest_3102 at 3281.303 kb on - strand, within Psest_3102 at 3281.303 kb on - strand, within Psest_3102 at 3281.338 kb on + strand, within Psest_3102 at 3281.338 kb on + strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.339 kb on - strand, within Psest_3102 at 3281.391 kb on - strand at 3281.644 kb on - strand at 3281.644 kb on - strand at 3281.644 kb on - strand at 3281.673 kb on + strand at 3281.673 kb on + strand at 3281.673 kb on + strand at 3281.673 kb on + strand at 3281.673 kb on + strand at 3281.673 kb on + strand at 3281.674 kb on - strand at 3281.674 kb on - strand at 3281.675 kb on + strand at 3281.675 kb on + strand at 3281.676 kb on - strand at 3281.676 kb on - strand at 3281.756 kb on + strand at 3281.756 kb on + strand at 3281.756 kb on + strand at 3281.756 kb on + strand at 3281.756 kb on + strand at 3281.757 kb on - strand at 3281.757 kb on - strand at 3281.757 kb on - strand at 3281.807 kb on + strand at 3281.807 kb on + strand at 3281.807 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 3,278,467 - +2.0 3,278,467 - +1.9 3,278,467 - -0.1 3,278,467 - -0.5 3,278,467 - +1.5 3,278,471 - -1.5 3,278,471 - -0.1 3,278,471 - -0.5 3,278,551 - Psest_3100 0.20 +0.8 3,278,551 - Psest_3100 0.20 +0.7 3,278,551 - Psest_3100 0.20 +1.5 3,278,551 - Psest_3100 0.20 +0.5 3,278,551 - Psest_3100 0.20 +1.5 3,278,576 - Psest_3100 0.24 -0.1 3,278,576 - Psest_3100 0.24 -0.1 3,278,584 - Psest_3100 0.26 +0.5 3,278,697 - Psest_3100 0.45 +0.0 3,278,697 - Psest_3100 0.45 -0.7 3,278,773 - Psest_3100 0.58 -1.7 3,278,800 - Psest_3100 0.63 +0.9 3,278,800 - Psest_3100 0.63 +1.5 3,278,802 - Psest_3100 0.63 +0.7 3,278,802 - Psest_3100 0.63 +0.2 3,278,841 - Psest_3100 0.70 +1.5 3,278,849 - Psest_3100 0.71 -0.5 3,278,849 - Psest_3100 0.71 +0.1 3,278,849 - Psest_3100 0.71 -0.5 3,278,931 - Psest_3100 0.85 +1.5 3,279,077 - -0.5 3,279,112 - +1.7 3,279,130 - -0.2 3,279,242 - -3.7 3,279,297 - +0.9 3,279,297 - -1.2 3,279,309 - +0.1 3,279,330 + -1.5 3,279,331 - +0.9 3,279,331 - -0.7 3,279,332 + +0.1 3,279,332 + -1.2 3,279,333 - -0.8 3,279,333 - -0.5 3,279,333 - -0.5 3,279,333 - -1.3 3,279,333 - +0.9 3,279,333 - +2.0 3,279,333 - +0.1 3,279,333 - -0.4 3,279,333 - +1.1 3,279,334 + -1.3 3,279,335 - -0.5 3,279,335 - -1.3 3,279,335 - -1.1 3,279,335 - -0.2 3,279,335 - +1.5 3,279,335 - -0.3 3,279,335 - +1.2 3,279,335 - -0.4 3,279,335 - +0.3 3,279,335 - +0.7 3,279,335 - -0.4 3,279,335 - -1.0 3,279,335 - -1.3 3,279,335 - -3.1 3,279,335 - -2.1 3,279,335 - +0.6 3,279,335 - -0.8 3,279,335 - -0.3 3,279,335 - +0.3 3,279,335 - -1.4 3,279,335 - -0.9 3,279,338 - -0.6 3,279,338 - -0.6 3,279,338 - -1.2 3,279,378 + -3.1 3,279,378 + -0.4 3,279,379 - +0.0 3,279,379 - -1.0 3,279,379 - -1.1 3,279,379 - -0.6 3,279,390 + -1.7 3,279,390 + -1.5 3,279,390 + +0.5 3,279,391 - -0.9 3,279,498 - +0.2 3,279,740 - Psest_3101 0.23 -0.2 3,279,911 + Psest_3101 0.34 -1.9 3,279,911 + Psest_3101 0.34 -1.1 3,279,912 - Psest_3101 0.34 -1.1 3,279,912 - Psest_3101 0.34 -0.6 3,279,912 - Psest_3101 0.34 -0.7 3,279,912 - Psest_3101 0.34 -1.0 3,279,912 - Psest_3101 0.34 -0.5 3,279,912 - Psest_3101 0.34 -0.3 3,279,912 - Psest_3101 0.34 +0.0 3,279,912 - Psest_3101 0.34 -0.6 3,279,912 - Psest_3101 0.34 -0.5 3,279,912 - Psest_3101 0.34 -0.4 3,279,912 - Psest_3101 0.34 -0.4 3,279,912 - Psest_3101 0.34 -1.2 3,279,996 - Psest_3101 0.39 -0.3 3,279,996 - Psest_3101 0.39 -2.5 3,279,996 - Psest_3101 0.39 +1.2 3,280,110 - Psest_3101 0.46 -0.5 3,280,292 + Psest_3101 0.57 -0.5 3,280,350 - Psest_3101 0.61 -0.5 3,280,350 - Psest_3101 0.61 -0.1 3,280,368 - Psest_3101 0.62 -1.7 3,280,368 - Psest_3101 0.62 -0.9 3,280,418 + Psest_3101 0.65 -2.0 3,280,419 - Psest_3101 0.65 -1.3 3,280,419 - Psest_3101 0.65 -0.5 3,280,419 - Psest_3101 0.65 -1.1 3,280,419 - Psest_3101 0.65 -0.9 3,280,475 + Psest_3101 0.69 -0.1 3,280,476 - Psest_3101 0.69 -1.2 3,280,476 - Psest_3101 0.69 -0.5 3,280,604 - Psest_3101 0.77 -1.2 3,280,713 - Psest_3101 0.83 +0.2 3,280,713 - Psest_3101 0.83 -0.2 3,280,713 - Psest_3101 0.83 -0.7 3,280,713 - Psest_3101 0.83 -0.1 3,280,713 - Psest_3101 0.83 -1.2 3,280,713 - Psest_3101 0.83 -1.1 3,280,713 - Psest_3101 0.83 -1.5 3,280,797 - Psest_3101 0.89 +0.0 3,280,797 - Psest_3101 0.89 -0.9 3,280,797 - Psest_3101 0.89 -0.7 3,280,805 + Psest_3101 0.89 -1.0 3,280,805 + Psest_3101 0.89 -0.6 3,280,806 - Psest_3101 0.89 -1.3 3,280,806 - Psest_3101 0.89 +0.5 3,280,806 - Psest_3101 0.89 -0.9 3,280,806 - Psest_3101 0.89 -0.4 3,280,806 - Psest_3101 0.89 -0.8 3,280,806 - Psest_3101 0.89 -1.3 3,280,806 - Psest_3101 0.89 -1.2 3,280,806 - Psest_3101 0.89 -1.1 3,280,806 - Psest_3101 0.89 -0.9 3,280,806 - Psest_3101 0.89 -1.1 3,280,806 - Psest_3101 0.89 -0.6 3,280,806 - Psest_3101 0.89 -1.3 3,280,806 - Psest_3101 0.89 +0.3 3,280,908 - -1.2 3,280,908 - -1.0 3,280,952 - -0.2 3,281,045 + Psest_3102 0.21 +0.6 3,281,045 + Psest_3102 0.21 -0.1 3,281,045 + Psest_3102 0.21 -1.2 3,281,045 + Psest_3102 0.21 +0.0 3,281,046 - Psest_3102 0.21 +0.7 3,281,046 - Psest_3102 0.21 -1.1 3,281,302 + Psest_3102 0.77 +1.1 3,281,302 + Psest_3102 0.77 -1.0 3,281,303 - Psest_3102 0.77 +0.7 3,281,303 - Psest_3102 0.77 +0.4 3,281,338 + Psest_3102 0.85 +1.1 3,281,338 + Psest_3102 0.85 -0.0 3,281,339 - Psest_3102 0.85 -0.9 3,281,339 - Psest_3102 0.85 -0.5 3,281,339 - Psest_3102 0.85 +0.1 3,281,339 - Psest_3102 0.85 +0.1 3,281,339 - Psest_3102 0.85 -0.8 3,281,339 - Psest_3102 0.85 +0.2 3,281,391 - +2.4 3,281,644 - +0.1 3,281,644 - -0.9 3,281,644 - -1.0 3,281,673 + -1.2 3,281,673 + -1.1 3,281,673 + -1.8 3,281,673 + -1.7 3,281,673 + +0.7 3,281,673 + -0.4 3,281,674 - -0.7 3,281,674 - -0.2 3,281,675 + -1.8 3,281,675 + -0.6 3,281,676 - +1.1 3,281,676 - +0.3 3,281,756 + +1.2 3,281,756 + +0.0 3,281,756 + +1.8 3,281,756 + +0.7 3,281,756 + -0.2 3,281,757 - -0.4 3,281,757 - +0.4 3,281,757 - -0.0 3,281,807 + -0.8 3,281,807 + -0.2 3,281,807 + +1.7
Or see this region's nucleotide sequence