Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0256

Experiment: D-Maltose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0254 and Psest_0255 are separated by 11 nucleotidesPsest_0255 and Psest_0256 overlap by 1 nucleotidesPsest_0256 and Psest_0257 overlap by 8 nucleotides Psest_0254: Psest_0254 - hypothetical protein, at 268,433 to 268,837 _0254 Psest_0255: Psest_0255 - RNA methyltransferase, RsmD family, at 268,849 to 269,463 _0255 Psest_0256: Psest_0256 - Predicted Zn-dependent peptidases, at 269,463 to 271,004 _0256 Psest_0257: Psest_0257 - Predicted Zn-dependent peptidases, at 270,997 to 272,349 _0257 Position (kb) 269 270 271 272Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 268.783 kb on + strand, within Psest_0254at 268.833 kb on - strandat 268.982 kb on + strand, within Psest_0255at 269.300 kb on + strand, within Psest_0255at 269.648 kb on - strand, within Psest_0256at 269.648 kb on - strand, within Psest_0256at 269.900 kb on - strand, within Psest_0256at 271.024 kb on + strandat 271.024 kb on + strandat 271.024 kb on + strandat 271.024 kb on + strandat 271.024 kb on + strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.025 kb on - strandat 271.058 kb on + strandat 271.059 kb on - strandat 271.276 kb on + strand, within Psest_0257at 271.731 kb on - strand, within Psest_0257at 271.850 kb on + strand, within Psest_0257

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Maltose (C)
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268,783 + Psest_0254 0.86 -1.1
268,833 - -2.9
268,982 + Psest_0255 0.22 +0.9
269,300 + Psest_0255 0.73 +1.9
269,648 - Psest_0256 0.12 -0.3
269,648 - Psest_0256 0.12 -0.8
269,900 - Psest_0256 0.28 +1.7
271,024 + +0.0
271,024 + +0.7
271,024 + -0.3
271,024 + +0.7
271,024 + -1.6
271,025 - -0.6
271,025 - -0.1
271,025 - +0.3
271,025 - +0.2
271,025 - +0.3
271,025 - +0.4
271,025 - +0.3
271,025 - +1.3
271,058 + -0.1
271,059 - -0.1
271,276 + Psest_0257 0.21 +0.1
271,731 - Psest_0257 0.54 +0.1
271,850 + Psest_0257 0.63 -0.3

Or see this region's nucleotide sequence