Private Fitness
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Specific Phenotypes for
Escherichia coli Nissle 1917
in phage experiments
Condition
Gene
Description
Fitness
Cons.
MOI 10
ECOLIN
_RS02490
nucleoside-specific channel-forming protein Tsx
variable
Yes
MOI 10
ECOLIN
_RS02810
molecular chaperone HtpG
variable
Yes
MOI 10
ECOLIN
_RS03730
phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)
-1.3
MOI 10
ECOLIN
_RS06225
flagellar hook assembly protein FlgD
+1.7
Yes
MOI 10
ECOLIN
_RS11090
flagellar basal body-associated protein FliL
variable
MOI 10
ECOLIN
_RS16115
single-stranded-DNA-specific exonuclease RecJ
-1.1
Yes
MOI 10
ECOLIN
_RS17845
DedA family general envelope maintenance protein YqjA
-1.7
Yes
MOI 10
ECOLIN
_RS18370
lipid asymmetry maintenance protein MlaB
variable
MOI 10
ECOLIN
_RS18375
phospholipid-binding protein MlaC
variable
MOI 10
ECOLIN
_RS18380
outer membrane lipid asymmetry maintenance protein MlaD
variable
MOI 10
ECOLIN
_RS18385
lipid asymmetry maintenance ABC transporter permease subunit MlaE
variable
MOI 10
ECOLIN
_RS18390
phospholipid ABC transporter ATP-binding protein MlaF
variable
Yes
MOI 10
ECOLIN
_RS19465
Tex family protein
variable
MOI 10
ECOLIN
_RS19520
HTH-type transcriptional regulator MalT
variable
Yes
MOI 10
ECOLIN
_RS20295
kdo(2)-lipid A phosphoethanolamine 7''-transferase
+1.9
Yes
MOI 10
ECOLIN
_RS21885
DNA helicase II
-1.7
MOI 10
ECOLIN
_RS22830
phosphoethanolamine transferase CptA
variable
MOI 10
ECOLIN
_RS23305
maltoporin LamB
+1.4
Yes
MOI 10
ECOLIN
_RS23805
two-component system response regulator BasR
+1.2
Phage
ECOLIN
_RS02750
efflux RND transporter permease AcrB
variable
Yes
Phage
ECOLIN
_RS04340
outer membrane protein OmpX
variable
Yes
Phage
ECOLIN
_RS04670
VirK/YbjX family protein
variable
Yes
Phage
ECOLIN
_RS04920
porin OmpF
variable
Yes
Phage
ECOLIN
_RS08730
multiple antibiotic resistance transcriptional regulator MarR
variable
Phage
ECOLIN
_RS12165
diguanylate cyclase
variable
Phage
ECOLIN
_RS15370
DUF3561 family protein
variable
Yes
Phage
ECOLIN
_RS15645
DUF423 domain-containing protein
variable
Yes
Phage
ECOLIN
_RS16165
aminomethyl-transferring glycine dehydrogenase
variable
Phage
ECOLIN
_RS16170
glycine cleavage system protein GcvH
variable
Phage
ECOLIN
_RS16415
pyridoxal phosphate homeostasis protein
variable
Phage
ECOLIN
_RS17745
transcriptional regulator EbgR
variable
Phage
ECOLIN
_RS17845
DedA family general envelope maintenance protein YqjA
variable
Phage
ECOLIN
_RS17850
EnvZ/OmpR regulon moderator MzrA
variable
Yes
Phage
ECOLIN
_RS19450
two-component system sensor histidine kinase EnvZ
variable
Phage
ECOLIN
_RS19455
two-component system response regulator OmpR
variable
Phage
ECOLIN
_RS19520
HTH-type transcriptional regulator MalT
variable
Phage
ECOLIN
_RS19850
phage sensitivity protein DcrB
variable
Yes
Phage
ECOLIN
_RS20190
cellulose synthase complex outer membrane protein BcsC
variable
Phage
ECOLIN
_RS20200
cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB
variable
Phage
ECOLIN
_RS20205
UDP-forming cellulose synthase catalytic subunit
variable
Phage
ECOLIN
_RS20210
cellulose biosynthesis protein BcsQ
variable
Phage
ECOLIN
_RS20215
cellulose biosynthesis protein BcsR
variable
Phage
ECOLIN
_RS20220
cellulose biosynthesis c-di-GMP-binding protein BcsE
variable
Phage
ECOLIN
_RS20230
cellulose biosynthesis protein BcsG
variable
Phage
ECOLIN
_RS21550
D-ribose pyranase
variable
Phage
ECOLIN
_RS21885
DNA helicase II
variable
Phage
ECOLIN
_RS21930
phospholipase A
variable
Phage
ECOLIN
_RS22905
TonB-dependent vitamin B12 receptor BtuB
variable
Phage
ECOLIN
_RS23285
maltose ABC transporter permease MalF
variable
Phage
ECOLIN
_RS23305
maltoporin LamB
variable
Phage
ECOLIN
_RS23310
maltose operon protein MalM
variable
Phage
ECOLIN
_RS24030
protein-disulfide reductase DsbD
variable
Phage
ECOLIN
_RS24510
autotransporter assembly complex protein TamA
variable
Phage
ECOLIN
_RS24515
autotransporter assembly complex protein TamB
variable
Phage
ECOLIN
_RS25205
energy-dependent translational throttle protein EttA
variable
Yes
Phage
ECOLIN
_RS26330
hypothetical protein
variable
Phage
ECOLIN
_RS26340
helix-turn-helix transcriptional regulator
variable