Specific Phenotypes for Klebsiella michiganensis M5al in nitrogen source experiments

Condition Gene Description Fitness Cons.
BWI76_RS18805 pyruvate:ferredoxin (flavodoxin) oxidoreductase -1.2  
BWI76_RS18835 nitrogenase iron-molybdenum cofactor biosynthesis protein NifE -1.5  
BWI76_RS18840 nitrogenase iron-molybdenum cofactor biosynthesis protein NifN -1.3  
BWI76_RS18860 homocitrate synthase -1.1  
BWI76_RS18890 nif-specific transcriptional activator NifA -1.2  
BWI76_RS18895 nitrogenase cofactor biosynthesis protein NifB -1.7  
0.1mM glass beads BWI76_RS17250 bifunctional acetaldehyde-CoA/alcohol dehydrogenase -1.5  
0.1mM glass beads BWI76_RS18805 pyruvate:ferredoxin (flavodoxin) oxidoreductase -1.0  
0.1mM glass beads BWI76_RS18850 iron-sulfur cluster assembly scaffold protein NifU -1.1  
0.1mM glass beads BWI76_RS26015 DUF2559 domain-containing protein +2.4  
Adenosine BWI76_RS10485 (hypo)xanthine hydroxylase reductase -2.3  
Adenosine BWI76_RS10570 cysteine ABC transporter substrate-binding protein -3.5  
Adenosine BWI76_RS10590 class V aminotransferase -2.3  
Adenosine BWI76_RS10595 allantoate amidohydrolase -2.1  
Cytidine BWI76_RS00765 DNA-binding transcriptional regulator CytR +1.5  
Cytidine BWI76_RS09580 surface protein -1.8  
Cytidine BWI76_RS10345 MFS transporter -1.5  
Cytidine BWI76_RS10775 pyrimidine utilization protein B -1.5  
Cytidine BWI76_RS18460 DgsA anti-repressor MtfA +1.8  
Cytidine BWI76_RS19610 Cytidine deaminase (EC 3.5.4.5) (from data) -3.4 Yes
Cytosine BWI76_RS06605 ammonium transporter -1.8  
Cytosine BWI76_RS12655 PTS cellobiose transporter subunit IIB -2.1  
D-Alanine BWI76_RS02700 D-serine/D-alanine/glycine transporter -1.1  
D-Alanine BWI76_RS06605 ammonium transporter -2.4 Yes
D-Alanine BWI76_RS06790 mechanosensitive channel MscK +2.1  
D-Alanine BWI76_RS17735 D-amino acid dehydrogenase small subunit -5.2 Yes
D-Alanine BWI76_RS24465 adenylate cyclase -2.4  
D-Serine BWI76_RS01920 alanine racemase +2.0  
D-Serine BWI76_RS02820 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase +2.5 Yes
D-Serine BWI76_RS04850 N-acetylmuramoyl-L-alanine amidase +2.5 Yes
D-Serine BWI76_RS23495 hypothetical protein +1.6  
D-Serine BWI76_RS23500 hypothetical protein +1.4  
D-Serine BWI76_RS27850 DNA-binding transcriptional regulator DsdC -4.3 Yes
D-Serine BWI76_RS27860 D-serine dehydratase -4.6 Yes
Gly-DL-Asp BWI76_RS05010 tRNA glutamyl-Q synthetase +1.4  
Gly-DL-Asp BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.8 Yes
Gly-DL-Asp BWI76_RS23870 Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) -2.9 Yes
Gly-DL-Asp BWI76_RS23880 Aminomethyltransferase (EC 2.1.2.10) (from data) -5.9 Yes
Gly-DL-Asp BWI76_RS26880 dipeptide ABC transporter permease DppB -2.0 Yes
Gly-DL-Asp BWI76_RS26885 ABC transporter substrate-binding protein -1.7 Yes
Glycine BWI76_RS00890 vitamin B12/cobalamin outer membrane transporter -1.4 Yes
Glycine BWI76_RS01685 methionine synthase -2.1 Yes
Glycine BWI76_RS21445 hypothetical protein +1.9 Yes
Glycine BWI76_RS23870 Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) -6.0 Yes
Glycine BWI76_RS23880 Aminomethyltransferase (EC 2.1.2.10) (from data) -5.2 Yes
Inosine BWI76_RS10495 LysR family transcriptional regulator -5.8  
Inosine BWI76_RS10510 OHCU decarboxylase -5.2  
Inosine BWI76_RS10540 allantoinase -5.9 Yes
Inosine BWI76_RS10550 AtzE family amidohydrolase -1.5  
Inosine BWI76_RS10570 cysteine ABC transporter substrate-binding protein -5.2  
Inosine BWI76_RS10590 class V aminotransferase -5.3  
Inosine BWI76_RS10595 allantoate amidohydrolase -5.8 Yes
Inosine BWI76_RS23270 phosphoenolpyruvate--protein phosphotransferase PtsP -1.5  
L-Alanine BWI76_RS00840 acetylornithine deacetylase -1.1  
L-Alanine BWI76_RS02700 D-serine/D-alanine/glycine transporter +1.2  
L-Alanine BWI76_RS17735 D-amino acid dehydrogenase small subunit -2.2 Yes
L-Alanine BWI76_RS18630 nitrogen assimilation transcriptional regulator +2.5  
L-Arginine BWI76_RS11670 Succinylornithine transaminase (EC 2.6.1.81) (from data) -6.8 Yes
L-Arginine BWI76_RS11685 Succinylarginine dihydrolase (EC 3.5.3.23) (from data) -6.5 Yes
L-Arginine BWI76_RS23270 phosphoenolpyruvate--protein phosphotransferase PtsP -1.6  
L-Aspartic Acid BWI76_RS02330 aspartate ammonia-lyase -2.9  
L-Aspartic Acid BWI76_RS08095 arginine ABC transporter ATP-binding protein -2.8 Yes
L-Aspartic Acid BWI76_RS08100 glutamate/aspartate transporter permease GltK -2.6 Yes
L-Aspartic Acid BWI76_RS08105 glutamate/aspartate ABC transporter permease GltJ -2.5 Yes
L-Aspartic Acid BWI76_RS14460 MurR/RpiR family transcriptional regulator +2.8  
L-Aspartic Acid BWI76_RS19175 dicarboxylate/amino acid:cation symporter -1.1  
L-Aspartic Acid BWI76_RS25905 hypothetical protein +2.0  
L-Citrulline BWI76_RS01925 aromatic amino acid aminotransferase -1.8  
L-Citrulline BWI76_RS11670 Succinylornithine transaminase (EC 2.6.1.81) (from data) -3.1 Yes
L-Citrulline BWI76_RS11685 Succinylarginine dihydrolase (EC 3.5.3.23) (from data) -2.7  
L-Citrulline BWI76_RS11990 exoribonuclease II +1.9  
L-Cysteine hydrochloride monohydrate BWI76_RS06575 cysteine synthase -3.3  
L-Cysteine hydrochloride monohydrate BWI76_RS25245 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase +1.5  
L-Glutamic acid monopotassium salt monohydrate BWI76_RS02330 aspartate ammonia-lyase -2.2  
L-Glutamic acid monopotassium salt monohydrate BWI76_RS08095 arginine ABC transporter ATP-binding protein -2.8  
L-Glutamic acid monopotassium salt monohydrate BWI76_RS08100 glutamate/aspartate transporter permease GltK -2.0  
L-Glutamic acid monopotassium salt monohydrate BWI76_RS08105 glutamate/aspartate ABC transporter permease GltJ -2.1 Yes
L-Glutamic acid monopotassium salt monohydrate BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.2  
L-Histidine BWI76_RS00575 DNA-binding protein +1.2  
L-Histidine BWI76_RS00840 acetylornithine deacetylase -2.7  
L-Histidine BWI76_RS08725 Formiminoglutamase (EC 3.5.3.8) (from data) -4.1 Yes
L-Histidine BWI76_RS08735 Urocanate hydratase (EC 4.2.1.49) (from data) -4.7 Yes
L-Histidine BWI76_RS08740 Histidine ammonia-lyase (EC 4.3.1.3) (from data) -4.8 Yes
L-Histidine BWI76_RS10290 transcriptional regulator -1.4  
L-Histidine BWI76_RS18630 nitrogen assimilation transcriptional regulator -3.7 Yes
L-Histidine BWI76_RS20915 peroxiredoxin -1.5  
L-Histidine BWI76_RS21905 class II aldolase -2.0  
L-Histidine BWI76_RS21910 glyoxylate/hydroxypyruvate reductase A -2.4  
L-Isoleucine BWI76_RS05980 ABC transporter ATP-binding protein -1.2  
L-Isoleucine BWI76_RS05995 branched-chain amino acid ABC transporter substrate-binding protein -1.5  
L-Isoleucine BWI76_RS17345 nitrate/nitrite two-component system sensor histidine kinase NarX -2.4  
L-Isoleucine BWI76_RS17735 D-amino acid dehydrogenase small subunit -2.2  
L-Isoleucine BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.5  
L-Isoleucine BWI76_RS26830 endoglucanase -1.5  
L-Leucine BWI76_RS00840 acetylornithine deacetylase -2.2  
L-Leucine BWI76_RS05985 branched-chain amino acid ABC transporter permease -1.5  
L-Leucine BWI76_RS17345 nitrate/nitrite two-component system sensor histidine kinase NarX -2.0  
L-Leucine BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.5  
L-Leucine BWI76_RS23535 hypothetical protein +1.6  
L-Leucine BWI76_RS23540 putative lysine exporter protein +1.4  
L-Leucine BWI76_RS24235 Branched-chain-amino-acid transaminase (EC 2.6.1.42) (from data) -1.7 Yes
L-Lysine BWI76_RS00840 acetylornithine deacetylase -1.4  
L-Lysine BWI76_RS04275 molybdopterin adenylyltransferase +1.7  
L-Lysine BWI76_RS09580 surface protein -3.5  
L-Lysine BWI76_RS11480 hypothetical protein +2.4  
L-Lysine BWI76_RS11485 serine protein kinase PrkA +2.1  
L-Lysine BWI76_RS11670 Succinylornithine transaminase (EC 2.6.1.81) (from data) -3.9 Yes
L-Lysine BWI76_RS11690 succinylglutamate desuccinylase -2.3  
L-Lysine BWI76_RS17740 SpoVR family protein +2.2  
L-Lysine BWI76_RS20320 histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP +1.4 Yes
L-Lysine BWI76_RS20325 histidine/lysine/arginine/ornithine ABC transporter permease HisM +1.5 Yes
L-Lysine BWI76_RS20330 histidine/lysine/arginine/ornithine ABC transporter permease HisQ +1.6 Yes
L-Lysine BWI76_RS20335 zinc transporter +1.5  
L-Lysine BWI76_RS20340 histidine ABC transporter substrate-binding protein HisJ +1.9 Yes
L-Lysine BWI76_RS23875 glycine cleavage system protein H +1.9  
L-Phenylalanine BWI76_RS01925 aromatic amino acid aminotransferase -2.5 Yes
L-Phenylalanine BWI76_RS17735 D-amino acid dehydrogenase small subunit -2.1 Yes
L-Phenylalanine BWI76_RS21485 DUF4154 domain-containing protein +1.4  
L-Proline BWI76_RS02330 aspartate ammonia-lyase -2.6  
L-Proline BWI76_RS02765 membrane protein +1.5  
L-Proline BWI76_RS05975 ABC transporter ATP-binding protein -1.7  
L-Proline BWI76_RS05980 ABC transporter ATP-binding protein -1.7  
L-Proline BWI76_RS05985 branched-chain amino acid ABC transporter permease -1.7  
L-Proline BWI76_RS05990 branched-chain amino acid ABC transporter permease -2.1  
L-Proline BWI76_RS05995 branched-chain amino acid ABC transporter substrate-binding protein -2.0  
L-Proline BWI76_RS10795 L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) -3.3 Yes
L-Proline BWI76_RS13050 transcriptional regulator variable  
L-Proline BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.6 Yes
L-Serine BWI76_RS20670 transcriptional regulator PtsJ -3.6  
L-Threonine BWI76_RS00330 molybdenum cofactor guanylyltransferase +1.5  
L-Threonine BWI76_RS00575 DNA-binding protein -2.0  
L-Threonine BWI76_RS04275 molybdopterin adenylyltransferase +1.9  
L-Threonine BWI76_RS05975 ABC transporter ATP-binding protein -1.9  
L-Threonine BWI76_RS05980 ABC transporter ATP-binding protein -2.5  
L-Threonine BWI76_RS05985 branched-chain amino acid ABC transporter permease -3.3  
L-Threonine BWI76_RS05990 branched-chain amino acid ABC transporter permease -2.2  
L-Threonine BWI76_RS05995 branched-chain amino acid ABC transporter substrate-binding protein -3.5  
L-Threonine BWI76_RS06000 ethanolamine ammonia-lyase small subunit -1.5  
L-Threonine BWI76_RS06005 ethanolamine ammonia lyase large subunit -1.2  
L-Threonine BWI76_RS08815 cyclic pyranopterin phosphate synthase +1.7  
L-Threonine BWI76_RS08835 molybdopterin guanine dinucleotide biosynthesis protein MoaE +1.9  
L-Threonine BWI76_RS17345 nitrate/nitrite two-component system sensor histidine kinase NarX -2.5  
L-Threonine BWI76_RS17385 nitrate regulatory protein +1.1  
L-Threonine BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.8  
L-Threonine BWI76_RS20495 alanine transaminase -1.6  
L-Threonine BWI76_RS23870 Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) -1.4 Yes
L-Threonine BWI76_RS23880 Aminomethyltransferase (EC 2.1.2.10) (from data) -1.5 Yes
L-Threonine BWI76_RS27255 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) -1.3  
L-Tryptophan BWI76_RS01925 aromatic amino acid aminotransferase -4.3  
L-Tryptophan BWI76_RS03535 Tryptophanase (EC 4.1.99.1) (from data) -1.4  
L-Tryptophan BWI76_RS05980 ABC transporter ATP-binding protein -2.9  
L-Tryptophan BWI76_RS05990 branched-chain amino acid ABC transporter permease -3.2  
L-Tryptophan BWI76_RS05995 branched-chain amino acid ABC transporter substrate-binding protein -2.7  
L-Tryptophan BWI76_RS06000 ethanolamine ammonia-lyase small subunit -1.8  
L-Tryptophan BWI76_RS06005 ethanolamine ammonia lyase large subunit -1.5  
L-Tryptophan BWI76_RS06125 D-alanine--D-alanine ligase A +1.2  
L-Tryptophan BWI76_RS11480 hypothetical protein +1.4  
L-Tryptophan BWI76_RS11485 serine protein kinase PrkA +1.6  
L-Tryptophan BWI76_RS17345 nitrate/nitrite two-component system sensor histidine kinase NarX -2.5  
L-Tryptophan BWI76_RS17740 SpoVR family protein +1.5  
L-Tryptophan BWI76_RS18630 nitrogen assimilation transcriptional regulator -2.7  
L-Tryptophan BWI76_RS26830 endoglucanase -2.4  
L-Valine BWI76_RS13005 thiamine pyrophosphate protein TPP-binding protein -1.2  
L-Valine BWI76_RS17735 D-amino acid dehydrogenase small subunit -2.6  
L-Valine BWI76_RS20495 alanine transaminase -1.4 Yes
L-tyrosine disodium salt BWI76_RS04315 Na+/H+ antiporter NhaA -3.0  
L-tyrosine disodium salt BWI76_RS17205 cardiolipin synthase A -2.6  
L-tyrosine disodium salt BWI76_RS26965 hypothetical protein -2.4  
N-Acetyl-D-Glucosamine BWI76_RS08220 PTS N-acetyl glucosamine transporter subunit IIABC -4.7 Yes
N-Acetyl-D-Glucosamine BWI76_RS10345 MFS transporter -1.2  
Parabanic Acid BWI76_RS24465 adenylate cyclase -2.3  
Parabanic Acid BWI76_RS24495 urease accessory protein -2.9 Yes
Parabanic Acid BWI76_RS24510 urease subunit alpha -2.6 Yes
Parabanic Acid BWI76_RS24515 urease accessory protein UreE -2.3 Yes
Parabanic Acid BWI76_RS24520 urease accessory protein UreF -3.3 Yes
Parabanic Acid BWI76_RS24525 urease accessory protein UreG -3.1 Yes
Putrescine Dihydrochloride BWI76_RS03500 hypothetical protein -1.0  
Putrescine Dihydrochloride BWI76_RS09465 polyamine ABC transporter ATP-binding protein -1.8 Yes
Putrescine Dihydrochloride BWI76_RS10690 gamma-glutamylputrescine oxidoreductase -3.2 Yes
Putrescine Dihydrochloride BWI76_RS10695 aldehyde dehydrogenase PuuC -3.2 Yes
Putrescine Dihydrochloride BWI76_RS10705 Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) (from data) -4.5 Yes
Putrescine Dihydrochloride BWI76_RS10710 Glutamate--putrescine ligase (EC 6.3.1.11) (from data) -3.2 Yes
Putrescine Dihydrochloride BWI76_RS10720 putrescine importer -2.9 Yes
Putrescine Dihydrochloride BWI76_RS20090 ribonuclease Z -3.1  
Sodium nitrate BWI76_RS06605 ammonium transporter -2.0 Yes
Sodium nitrate BWI76_RS08925 DNA-binding protein YbiB -3.9 Yes
Sodium nitrate BWI76_RS17365 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) -4.3 Yes
Sodium nitrate BWI76_RS17380 nitrate transporter component -5.0 Yes
Sodium nitrate BWI76_RS17385 nitrate regulatory protein -3.9 Yes
Sodium nitrate BWI76_RS23070 cobalt-precorrin-7 (C(5))-methyltransferase -4.5  
Thymidine BWI76_RS01060 guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase -1.5 Yes
Thymidine BWI76_RS04135 thymidine phosphorylase +1.2 Yes
Thymidine BWI76_RS08780 ABC transporter +1.4 Yes
Thymidine BWI76_RS08815 cyclic pyranopterin phosphate synthase +1.5  
Thymidine BWI76_RS09175 DNA-binding transcriptional regulator -1.8  
Thymidine BWI76_RS10760 nitroreductase family protein -4.4  
Thymidine BWI76_RS10775 pyrimidine utilization protein B -4.5  
Thymidine BWI76_RS10785 transcriptional regulator +2.3 Yes
Thymidine BWI76_RS17380 nitrate transporter component +1.3  
Thymidine BWI76_RS17385 nitrate regulatory protein +1.6 Yes
Thymidine BWI76_RS26130 ADP compounds hydrolase NudE -2.9  
Thymine BWI76_RS00330 molybdenum cofactor guanylyltransferase +2.1  
Thymine BWI76_RS01060 guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase -2.3 Yes
Thymine BWI76_RS02570 transcriptional regulator +1.8  
Thymine BWI76_RS03355 hypothetical protein -1.5  
Thymine BWI76_RS04275 molybdopterin adenylyltransferase +2.2  
Thymine BWI76_RS08815 cyclic pyranopterin phosphate synthase +2.4  
Thymine BWI76_RS08835 molybdopterin guanine dinucleotide biosynthesis protein MoaE +1.9  
Thymine BWI76_RS10785 transcriptional regulator +3.0 Yes
Thymine BWI76_RS17380 nitrate transporter component +1.7  
Thymine BWI76_RS17385 nitrate regulatory protein +2.1 Yes
Thymine BWI76_RS23270 phosphoenolpyruvate--protein phosphotransferase PtsP +1.8 Yes
Urea BWI76_RS24465 adenylate cyclase -1.8  
Uridine BWI76_RS00765 DNA-binding transcriptional regulator CytR +4.5  
Uridine BWI76_RS00890 vitamin B12/cobalamin outer membrane transporter +1.2  
Uridine BWI76_RS01295 Uridine phosphorylase (EC 2.4.2.3) (from data) -5.1  
Uridine BWI76_RS06060 DNA-binding response regulator variable  
Uridine BWI76_RS10760 nitroreductase family protein -5.0  
Uridine BWI76_RS10775 pyrimidine utilization protein B -6.4 Yes
sand BWI76_RS17250 bifunctional acetaldehyde-CoA/alcohol dehydrogenase -2.3  
sand BWI76_RS18835 nitrogenase iron-molybdenum cofactor biosynthesis protein NifE -1.3  
sand BWI76_RS18840 nitrogenase iron-molybdenum cofactor biosynthesis protein NifN -1.3  
sand BWI76_RS18850 iron-sulfur cluster assembly scaffold protein NifU -1.3